Keeping Cells Alive in Microscopy

Biophysica, Journal Year: 2025, Volume and Issue: 5(1), P. 1 - 1

Published: Jan. 6, 2025

Light microscopy has emerged as one of the fundamental methods to analyze biological systems; novel techniques 3D and super-resolution (SRM) with an optical resolution down sub-nanometer range have recently been realized. However, most these achievements made fixed specimens, i.e., direct information about dynamics biosystem studied was not possible. This stimulated development live cell imaging approaches, including Low Illumination Fluorescence Microscopy, Sheet (Fluorescence) Microscopy (LSFM), or Structured (SIM). Here, we discuss perspectives, methods, relevant light doses advanced fluorescence keep cells alive at low levels phototoxicity.

Language: Английский

Microscopy at a glance: New poster article series exploring the intersection of art, science and imaging

Journal of Microscopy, Journal Year: 2024, Volume and Issue: 296(2), P. 111 - 114

Published: Oct. 9, 2024

Language: Английский