A meta-analysis of age-dependent changes in extracellular vesicle proteins in C. elegans DOI Creative Commons
Prasun Kumar Bhunia, Prasad Kasturi

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Extracellular vesicles (EVs) contribute to the maintenance of organism-wide proteostasis by mediating intercellular communication. Loss and altered communication are associated with aging age-related diseases, suggesting key roles for EVs. However, it is unclear how proteome EVs changes age. To identify EV-associated proteins (EVAPs) their fate age, we curated publicly available data from C. elegans model organism. Our analysis reveals that carry involve protein quality control. We found abundance EV significantly Many these also aggregate age overlap Aβ driven aggregates. observe a subset alter in response heat stress pathogen infections Further, human orthologs EVAPs brain tissues affected Alzheimer’s disease. This meta-analysis highlights composition, changes, aggregation during aging, disease conditions. Overall, this study provides new insights into dynamics may possibly help identifying potential biomarkers diseases.

Language: Английский

Exosomes in Regulating miRNAs for Biomarkers of Neurodegenerative Disorders DOI
Azhagu Madhavan Sivalingam,

Darshitha D Sureshkumar

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 7, 2025

Language: Английский

Citations

1

Revisiting the advance of age-dependent α-synuclein propagation and aggregation DOI Open Access
Dong‐Yan Song, Jiayi Li

Ageing and Neurodegenerative Diseases, Journal Year: 2025, Volume and Issue: 5(1)

Published: Feb. 22, 2025

Aging is a major risk factor for different neurodegenerative diseases (NDDs), including Parkinson’s disease (PD). In PD, one of the key neuropathological features cytoplasmic protein aggregation, named Lewy bodies (LBs) in cell body, and neurites (LNs) neuronal processes terminals. The α-synuclein (α-syn) has been found to be component LBs LNs, considered play central role their formation. α-Syn also increases healthy aging conditions. Evidence shown that promotes α-syn pathological aggregation propagation and, therefore, may induce aggravate PD pathogenesis. Here, we aim highlight recent advances age-related prion-like discuss subsequent consequences functions.

Language: Английский

Citations

0

Membrane Charge Drives the Aggregation of TDP-43 Pathological Fragments DOI Creative Commons
Giacomo Corucci, Devkee M. Vadukul, Nicolò Paracini

et al.

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

TDP-43 protein is an RNA-binding linked to amyotrophic lateral sclerosis, frontotemporal dementia, and Alzheimer disease. While normally a that shuttles between the nucleus cytoplasm, has recently been found also in extracellular vesicles. These are important medium for cell-cell communication allows transfer of lipids, proteins, genetic material among cells. An increasing concern neurodegenerative diseases, however, possibility vesicles can provide effective way spread misfolded proteins could "infect" other cells according "prion-like" mechanism. To characterize interaction with lipid membranes, we carried out systematic biophysical study using fragment lacking first 84 N-terminal residues, called M85, synthetic model phospholipid membranes. We utilized standard techniques, such as fluorescence microscopy, complemented by neutron reflectivity measurements. Our results show charge affects modality which M85 interacts membranes: higher negative induces bind bilayer surface, promoting aggregation decreasing damage this causes. Thus, speculate M85-lipid membrane play previously undefined role TDP-43-related diseases.

Language: Английский

Citations

0

A case for the study of native extracellular vesicles DOI Creative Commons
Dhanya K. Nambiar, Quynh‐Thu Le, Ferdinando Pucci

et al.

Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 14

Published: July 12, 2024

Three main areas of research revolve around extracellular vesicles (EVs): their use as early detection diagnostics for cancer prevention, engineering EVs or other enveloped viral-like particles therapeutic purposes and to understand how impact biological processes. When investigating the biology EVs, it is important consider strategies able track alter directly

Language: Английский

Citations

1

A meta-analysis of age-dependent changes in extracellular vesicle proteins in C. elegans DOI Creative Commons
Prasun Kumar Bhunia, Prasad Kasturi

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 28, 2024

Abstract Extracellular vesicles (EVs) contribute to the maintenance of organism-wide proteostasis by mediating intercellular communication. Loss and altered communication are associated with aging age-related diseases, suggesting key roles for EVs. However, it is unclear how proteome EVs changes age. To identify EV-associated proteins (EVAPs) their fate age, we curated publicly available data from C. elegans model organism. Our analysis reveals that carry involve protein quality control. We found abundance EV significantly Many these also aggregate age overlap Aβ driven aggregates. observe a subset alter in response heat stress pathogen infections Further, human orthologs EVAPs brain tissues affected Alzheimer’s disease. This meta-analysis highlights composition, changes, aggregation during aging, disease conditions. Overall, this study provides new insights into dynamics may possibly help identifying potential biomarkers diseases.

Language: Английский

Citations

0