The age-related characteristics of neurophysiological and ultrasound parameters of the facial nerve in healthy children

Russian neurological Journal, Journal Year: 2025, Volume and Issue: 29(5), P. 55 - 61

Published: Jan. 7, 2025

Introduction . Neurophysiological methods, such as nerve conduction studies (NCS) and transcranial magnetic stimulation (TMS), well ultrasound (US), can be used to examine patients with facial neuropathy. Currently, normative instrumental parameters exist only for adults have not been studied in children. We provided age-related reference values NCS, TMS US of the healthy Objective The determination age-specifi c neurophysiological Material methods TMS, were 65 children aged 5 18 without neurological symptoms. In addition, results NCS under (n = 24) analyzed retrospectively. Statistical analysis was processed an assessment normality distribution use parametric or nonparametric tests. Results An increase amplitude M-wave revealed m. nasalis orbicularis oculi, amplitude, latency area motor evoked potentials (MEP) frontalis, orbicularis, nasalis. According US, there no signifi cant diff erences diameter depth between 5–12 12–18. Conclusion. obtained erent age groups both everyday clinical research practices.

Language: Английский

Claudin-11, a hypomyelinating leukodystrophy 22 (HLD22)-responsible protein, uniquely interacts with shroom-2 to change cell phenotypes

BBA Advances, Journal Year: 2025, Volume and Issue: unknown, P. 100159 - 100159

Published: March 1, 2025

Oligodendroglial cells are a type of glial cell in the central nervous system (CNS) that wrap neuronal axons with differentiated plasma membranes known as myelin sheaths. While physiological functions oligodendrocytes, such generating saltatory conduction and protecting axons, well understood, and/or pathophysiological molecular mechanisms governing their differentiation before myelination remain unclear. In this study, we describe for first time claudin-11, protein associated hypomyelinating leukodystrophy 22 (HLD22), interacts shroom-2, presumable adaptor containing PSD95, DLG1, ZO-1 (PDZ) domain. Knockdown claudin-11 using specific siRNA resulted decrease morphological changes marker proteins FBD-102b oligodendroglial model undergoing differentiation. Transfection C-terminal PDZ ligand sequence which was found to interact domain also reduced these phenotypic changes. The HLD22-associated mutated failed shroom-2. Furthermore, knockdown shroom-2 or transfection is involved interaction decreased expression. These were linked phosphorylation states Akt kinase, key signaling molecule myelination. results suggest between plays role shaping morphology, providing insights into underlying myelination, potential pathological HLD22 at cellular levels.

Language: Английский

Bcl2l12, a novel protein interacting with Arf6, triggers Schwann cell differentiation program

The Journal of Biochemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 6, 2024

Schwann cells are glial in the peripheral nervous system (PNS); they wrap neuronal axons with their differentiated plasma membranes called myelin sheaths. Although physiological functions, such as generating saltatory conduction, have been well studied PNS, molecular mechanisms by which undergo differentiation program without apparent morphological changes before dynamic sheath formation remain unclear. Here, for first time, we report that Arf6, a small GTP/GDP-binding protein controlling differentiation, and guanine-nucleotide exchange factors cytohesin proteins involved regulation of cell marker expression primary cells. Specific inhibition Arf6 cytohesins NAV-2729 SecinH3, respectively, decreased 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) fibrillary acidic (GFAP). Similar results using promoter assays were observed IMS32 line. Furthermore, an affinity-precipitation technique, identified Bcl2-like 12 (Bcl2l12) novel GTP-bound Arf6-interacting protein. Knockdown Bcl2l12 specific artificial miRNA proteins. The knockdown also led to filamentous actin extents. These suggest can trigger providing evidence relay underlies how differentiate.

Language: Английский