Exosome‐derived long non‐coding RNA AC010789.1 modified by FTO and hnRNPA2B1 accelerates growth of hair follicle stem cells against androgen alopecia by activating S100A8/Wnt/β‐catenin signalling

Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 1, 2025

Abstract Background The increased incidence of androgenic alopecia (AGA) causes adverse physiological and psychological effects on people all genders. hair follicle stem cells (HFSCs) have displayed clinical improvements AGA. However, the molecular mechanism HFSCs against AGA remains elusive. Methods expression prognosis lncRNA AC010789.1 in tissues were assessed by qRT‐PCR analysis. CCK‐8, EdU Transwell analysis utilized to assess cell growth. specific binding between FTO mediated m 6 A modification or effect hnRNPA2B1, S100A8 Wnt/β‐catenin signaling was confirmed bioinformatic analysis, RIP, RNA pull‐down Western blot assay. Exosome‐loaded prompted proliferation regeneration hairless mice. Results We herein found that mRNA levels decreased tissue samples but surrounding normal samples. Overexpression (OE) promoted HFSC proliferation, DNA synthesis migration as well K6HF Lgr5 upregulation, whereas knockdown showed opposite effects. total reduced demethylase upregulated samples, these indicated reverse results OE its abolished AC010789.1‐induced proliferation. In addition, identified bind reader which downregulated hnRNPA2B1 attenuated knockdown‐induced inhibition Moreover, could enhance downstream protein expression, accelerate Conclusions Our findings demonstrated exosome‐derived modified facilitated human activating S100A8/Wnt/β‐catenin signaling. Key points Long non‐coding (lncRNA) from (HFSCs). LncRNA HFSCs. FTO/hnRNPA2B1‐mediated growth signalling. Exosome‐derived accelerated

Language: Английский

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Effectiveness of Exosome Treatment in Androgenetic Alopecia: Outcomes of a Prospective Study

Aesthetic Plastic Surgery, Journal Year: 2024, Volume and Issue: 48(21), P. 4262 - 4271

Published: Aug. 22, 2024

Harnessing the regenerative capabilities of stem cell-derived exosomes holds great promise for developing novel hair growth therapies, offering hope individuals experiencing loss or alopecia. This aimed to elucidate effect "foreskin-derived mesenchymal stromal cells derived exosome" injection into scalp on density in patients with androgenetic alopecia and contribution this treatment patient satisfaction.

Language: Английский

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Explorando a realidade dos exossomos na Dermatologia

Published: Jan. 1, 2025

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Angelica sinensis and Ligusticum sinense ‘Chuanxiong’ Leaf Essential Oils Promote Hair Growth without Acute Toxicity

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(4)

Published: April 1, 2025

Background Rhizome Angelica sinensis and Ligusticum sinense ‘chuanxiong’ are the main medicinal herbs used in traditional Chinese or Asian medicine to treat alopecia likely associated with their expertise invigorating blood circulation. However, effects of volatile essential oils present aboveground parts unclear. Objective To evaluate general safety from A. L. leaves hair grow promoting effect on model mice cells dehydrotestosterone-induced androgenetic alopecia. Methods Essential oil was evaluated based acute oral/dermal/eye irritation toxicity tests laboratory animals. (5%, 3% 1%) were topically applied for 28 days. They (50-200 mg/L) appended monoculture 48 h. Results Both showed no toxicity. Mice dorsal skin indicated faster regrowth initiated by than minoxidil. Histological analysis shows that both increased follicle number dermal thickness treated dehydrotestosterone, 1% performing best. The exhibited an average follicles (pcs/field view) 69.73 70.13, respectively, which significantly larger (14.80) minoxidil (36.53) mice. reversed inhibition secretion growth promotors, vascular endothelial factor keratinocyte factor, cultured cells. Conclusion may act as safe effective adjuvant agents aromatherapy cosmetics human loss.

Language: Английский

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Exosomes in Dermatological Research: Unveiling Their Multifaceted Role in Cellular Communication, Healing, and Disease Modulation

Cosmetics, Journal Year: 2025, Volume and Issue: 12(1), P. 16 - 16

Published: Jan. 22, 2025

Exosomes, a subtype of extracellular vehicles (EVs), play pivotal role in cellular communication and have gained considerable attention dermatological research. Formed through the inward budding endosomal membrane, exosomes facilitate transfer proteins, lipids, nucleic acids, including microRNAs (miRNAs), thereby influencing behavior function recipient cells. These vesicles are secreted by various cell types, keratinocytes, crucial for maintaining skin homeostasis, regulating immune responses, promoting wound healing. Exosomes demonstrated therapeutic potential addressing conditions such as hair disorders, cancers photoaging enhanced regeneration reduced oxidative stress. However, they also implicated disease progression, with pathogens utilizing exosome release to evade host responses. Recent studies highlight diverse origins functions exosomes, suggesting their promise innovative agents dermatology. As research continues elucidate multifaceted roles, represent frontier understanding intercellular developing novel treatments skin-related diseases, underscoring impact on both health clinical applications. This review synthesizes existing literature biology isolation focus implications contexts.

Language: Английский

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Extracellular vesicles and bioactive peptides for regenerative medicine in cosmetology

Ageing Research Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 102712 - 102712

Published: March 1, 2025

Language: Английский

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A Prospective Study of Exosome Therapy for Androgenetic Alopecia

Aesthetic Plastic Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

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Exploring the reality of exosomes in dermatology

Anais Brasileiros de Dermatologia, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Exosomes are extracellular nanovesicles secreted by several cells in the human and animal body. Consisting of a lipid membrane encapsulated proteins, they contain biologically active substances such as DNA, RNA, transcription factors, metabolites. Discovered 1980s, exosomes play an important role cell-to-cell communication immune function. They vary size, content, function depending on cell origin. have attracted interest field Dermatology due to their potential applications treatment scars, skin rejuvenation, hair regeneration, other dermatological conditions. However, further clinical studies needed prove efficacy safety. Regulatory issues also need be considered, use cosmetics medical treatments is not yet fully approved some countries. Moreover, it understand risks side effects associated with before use. Although promising, more research explore full Medicine Dermatology.

Language: Английский

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Medical Treatment for Androgenetic Alopecia

Facial Plastic Surgery, Journal Year: 2023, Volume and Issue: 40(02), P. 252 - 266

Published: Oct. 23, 2023

Abstract Androgenetic alopecia is a common type of hair loss, which generally influenced by genetic factors and systemic androgens resulting in follicular miniaturization.1 It can cause cosmetic problems leading to psychological distress among affected men women. Effective standard medical treatments available are topical minoxidil 2 5%, oral finasteride, dutasteride, transplantation.1 However, some patients do not achieve favorable results with treatments. For these reasons, other novel have been developed, including new medications, regenerative medicines (autologous platelet-rich plasma, adipose-derived stem cells, micrograft generation, exosome), low-level laser therapy

Language: Английский

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Efficacy of Placental-Derived Mesenchymal Stem Cell Exosome Therapy in Treating Androgenetic Alopecia: A Clinical Trial Study

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Background: Androgenetic alopecia (AGA) is characterized by the miniaturization of hair follicles, leading to thinning and loss. Existing treatments are limited, stem cell-derived exosome therapy has emerged as a potential alternative. This study aimed evaluate efficacy placenta-derived mesenchymal cell (P-MSC) exosomes for treating AGA. Methods: This phase I/II clinical trial included 12 patients aged 18-60. Exosomes were isolated from cells harvested placentas consenting donors injected into scalp every 14 days 2 months. Outcome measures density, diameter, loss count, assessed at baseline, 3 weeks, 6 weeks post-intervention. Data analyzed using repeated measure ANOVA with STATA version 14.2. Results: At mean density was 96.5 hairs/cm², diameter 0.049 mm, count 200 hairs. Post-intervention, significantly increased 111.7 hairs/cm² 163.5 weeks. Hair also 0.058 mm 0.059 Mean decreased 80 hairs No significant adverse effects or changes in laboratory findings observed. Conclusions: Placenta-derived demonstrated improvements reduced androgenetic alopecia. Further controlled trials larger sample sizes longer follow-up periods warranted validate these explore molecular mechanisms involved.

Language: Английский

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