Materials Today Bio,
Journal Year:
2025,
Volume and Issue:
32, P. 101750 - 101750
Published: April 17, 2025
Osteoporotic
fractures
typically
exhibit
delayed
healing
due
to
impaired
cell
recruitment,
chronic
inflammation,
and
disrupted
neurovascular
signaling.
Sensory
nerve
signaling
plays
a
crucial
role
in
fracture
repair,
its
deficiency
is
significant
factor
leading
healing.
Addressing
these
deficiencies
overcoming
the
challenges
associated
with
bone
repair
osteoporosis.
In
this
study,
smart
composite
hydrogel
(denoted
as
OCS-MPC)
was
synthesized
by
embedding
CGRP-functionalized
polydopamine-coated
MXene
nanosheets
(MXene/PDA/CGRP)
into
boronic
acid-modified
oxidized
hyaluronic
acid-crosslinked
carboxymethyl
chitosan
(OHA-PBA/CMCS)
loaded
SDF-1.
OCS-MPC
enables
controlled
release
of
SDF-1
CGRP,
aiming
promote
early
callus
formation
late-stage
remodeling
osteoporotic
fractures.
Due
dynamic
crosslinking
via
imine
borate
ester
bonds,
exhibits
rapid
gelation,
injectability,
self-healing
properties.
vitro
experiments
demonstrated
excellent
osteogenic,
angiogenic,
neurogenic
properties
hydrogel.
vivo
studies
using
an
femoral
model
showed
that
enhanced
MSCs
recruitment
SDF-1/CXCR4
axis,
significantly
improving
stages
repair.
Additionally,
promoted
mineralization
later
through
enhancing
CGRP
Immunofluorescence
analysis
further
confirmed
increased
expression
TUBB3,
CD31,
indicating
successful
regeneration
network.
These
findings
highlight
potential
addressing
both
healing,
providing
promising
therapeutic
strategy
for
patients.
Biomolecules,
Journal Year:
2025,
Volume and Issue:
15(5), P. 682 - 682
Published: May 8, 2025
Melatonin
(N-acetyl-5-methoxytryptamine)
is
a
hormone
associated
with
the
regulation
of
biological
rhythms.
The
indoleamine
secreted
by
pineal
gland
during
night,
following
circadian
rhythm.
highest
plasmatic
levels
are
reached
whereas
lowest
achieved
day.
In
addition
to
gland,
other
organs
and
tissues
also
produce
melatonin,
like,
for
example,
retina,
Harderian
glands,
gut,
ovaries,
testes,
skin,
leukocytes,
or
bone
marrow.
list
extensive,
including
cerebellum,
airway
epithelium,
liver,
kidney,
adrenals,
thymus,
thyroid,
pancreas,
carotid
body,
placenta,
endometrium.
At
all
these
locations,
availability
melatonin
intended
local
use.
Interestingly,
decline
amplitude
secretion
occurs
in
old
subjects
comparison
that
found
younger
subjects.
Moreover,
genetic
environmental
factors
primary
causes
diseases,
oxidative
stress
key
contributor
most
pathologies.
Numerous
studies
exist
show
interesting
effects
different
models
disease.
Impairment
its
might
have
deleterious
consequences
cellular
physiology.
this
regard,
natural
compound
carrier
not
yet
completely
known
potential
deserves
consideration.
Thus,
has
emerged
as
helpful
ally
could
be
considered
guard
powerful
tools
orchestrate
homeostasis
majorly
based
on
antioxidant
effects.
review,
we
provide
an
overview
widespread
actions
against
diseases
preferentially
affecting
elderly.
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(10)
Published: May 19, 2025
ABSTRACT
While
adipose‐derived
stem
cells
(ADSCs)
transplantation
represents
an
appealing
therapeutic
strategy
for
bone
defect
repair,
the
osteogenic
capacity
of
ADSCs
is
largely
limited.
Melatonin
has
been
demonstrated
to
contribute
marrow
cell
(BMSC)
osteogenesis.
However,
its
effect
on
differentiation
not
yet
determined.
This
study
aims
identify
whether
melatonin
exerts
influences
in
rat
ADSCs.
Rat
were
isolated
and
identified.
Subsequently,
impact
proliferation
was
examined.
The
effects
phenotypic
features
as
well
marker
genes
proteins
determined
through
use
alkaline
phosphatase
(ALP)
staining,
ALP
activity
assay,
alizarin
red
staining
(ARS),
RT‐qPCR,
western
blot
cellular
immunofluorescence
assay.
To
investigate
potential
molecular
mechanism
which
promotes
ADSCs,
RNA
sequencing,
MAPK
signaling
pathway
blocking
assay
p38
mRNA
interference
carried
out.
results
showed
that
at
concentrations
0–100
μM
safe
nontoxic
with
concentration
100
exhibiting
most
pronounced
Additionally,
observed
activate
p38/MAPK
Moreover,
inhibitor
(SB203580)
siRNA
targeting
(p38
siRNA)
found
inhibit
melatonin‐promoted
In
conclusion,
this
indicate
activation
pathway.
light
these
findings,
treatment
effective
promoting
Journal of Pineal Research,
Journal Year:
2025,
Volume and Issue:
77(3)
Published: April 1, 2025
ABSTRACT
Human
dental
pulp
stem
cells
(hDPSCs)
exhibit
amazing
therapeutic
abilities
in
a
variety
of
diseases
due
to
their
remarkable
self‐renewal
capacity
and
multi‐differentiation
potential.
However,
potential
could
be
weakened
by
various
factors
such
as
oxidative
stress
cell
survival
microenvironment
In
Vivo.
Here,
we
explored
the
protective
effect
mechanism
melatonin
(Mel)
on
hDPSCs
transplanted
type
1
diabetes
mellitus
(T1DM)
rat
model.
Nicotinamide
adenine
dinucleotide
(NAD
+
)
metabolism
mitochondrial
function
were
remarkably
impaired
T1DM
rats
caused
stress,
while
combination
Mel
post‐hDPSCs
transplantation
rebalance
NAD
homeostasis
through
regulating
NAMPT‐NAD
‐SIRT1
axis.
Furthermore,
significantly
reduced
intracellular
reactive
oxygen
species,
alleviated
senescence
apoptosis
exposed
hydrogen
peroxide
ameliorating
depletion
dysfunction.
The
role
extremely
essential
tissue
engineering
regenerative
medicine.
Experimental Physiology,
Journal Year:
2024,
Volume and Issue:
110(2), P. 261 - 276
Published: Nov. 29, 2024
Osteoporosis
(OP)
is
a
prevalent
bone
disease
characterized
by
reduced
density
and
quality,
increasing
fragility
fracture
risk.
Osteoclast
(OC)
activity
circadian
rhythm
play
role
in
the
pathogenesis
of
OP.
Melatonin
regulator
that
affects
metabolism,
but
its
molecular
mechanism
has
not
been
studied
detail.
This
study
aimed
to
identify
relationship
between
melatonin-related
genes
OP
through
bioinformatics
methods
verify
it
experimentally.We
analysed
microarray
data
from
GSE35959
dataset,
identifying
differentially
expressed
patients.
Circadian
rhythm-related
intersect
with
these
genes,
highlighting
CSNK1D
central
gene.
Functional
enrichment,
correlation
protein-protein
interaction
analyses
were
conducted.
Experimental
validation
involved
vitro
differentiation
assays
using
RAW264.7
cells
vivo
studies
an
ovariectomy-induced
rat
model
evaluate
osteoclastogenesis
effect
on
Differential
expression
analysis
revealed
272
significant
identified
as
melatonin
interplay.
showed
involvement
OC
inflammatory
pathways.
experiments
confirmed
upregulation
during
differentiation,
small
interfering
RNA-mediated
knockdown
marker
TRAP+
cell
formation.
vivo,
associated
loss
rats.
Melatonin-related
promotes
development
These
findings
suggest
potential
therapeutic
target
for
OP,
offering
insights
into
new
treatment
strategies
integrating
regulation.
