PARAMETERS OF INTACT PERIODONTIUM THAT ARE DETECTED BY CLINICAL, FUNCTIONAL, AND RADIOLOGICAL METHODS OF INVESTIGATION

The actual problems in dentistry, Journal Year: 2025, Volume and Issue: 20(4), P. 46 - 54

Published: Feb. 10, 2025

Subject are the parameters of intact periodontium, recorded using clinical, functional and radiological methods research. Objectives to analyze systematize up-to-date information concerning research that record periodontal range their values for periodontium. Methodology. The study was conducted within framework project “Development a method recreating structures bioequivalents obtained by three-dimensional bioprinting”, search analysis data scientific articles performed via international electronic databases eLibrary, PubMed, Google Scholar, Web Science, ScienceDirect from 2019 2024, keywords: “periodontium”, “clinical methods”, “functional “radiological “periodontal indices”, “gingival sulcus probing”, “intraoral scanning”. Results. 65 out 312 found performed. Among clinical methods, following were distinguished: diagnostic (questioning, inspection, palpation, measurement gingival thickness, width keratinized gingiva, height interdental papillae, depth recession, bleeding on probing, probing depth, attachment level, epithelial surface area inflamed area, etc.), treatment, dynamic observation; among – laser Doppler flowmetry, electromyography, rheography, periotestometry, perioscopy, etc.; radiation orthopantomography, occlusal radiography, cone beam computed tomography, ultrasound examination, etc. parameter periodontium given. Conclusions. measured in studies reflect its anatomical physiological characteristics state at given time; as rule, there is normal each parameter. same can be several methods. Currently, various used studies, choice which particular depends primary secondary outcomes, concept study.

Language: Английский

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Response of Alzheimer's disease-like triple transgenic 3x-Tg mice to experimental migraine evoked by nitroglycerin

Biomedicine & Pharmacotherapy, Journal Year: 2025, Volume and Issue: 184, P. 117920 - 117920

Published: Feb. 21, 2025

Language: Английский

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Optimizing Confocal Imaging Protocols for Muscle Fiber Typing in the Mouse Masseter Muscle

BIO-PROTOCOL, Journal Year: 2025, Volume and Issue: 15(1368)

Published: Jan. 1, 2025

The masseter muscle, a key orofacial demonstrates unique anatomical and functional properties, including sexual dimorphism in myosin heavy chain (MyHC) expression complex fiber architecture. Despite its importance mastication relevance to various disorders, phenotypic characterization of the remains limited. Conventional fluorescence microscopy has been cornerstone muscle typing, reliably identifying MyHC isoforms measuring cross-sectional areas. Building on this foundation, confocal offers complementary advantages, such as enhanced resolution, increased flexibility for multiplexing, ability visualize structures three dimensions. This study presents detailed protocol using achieve high-resolution imaging molecular cryosections. By leveraging advanced technologies white light lasers extended z-length imaging, method ensures precise spectral separation, simultaneous multichannel detection, capture architecture includes tissue preparation, immunostaining isoforms, postprocessing segmentation quantification. setup was optimized minimizing signal bleed through, improving signal-to-noise ratio, enabling visualization fibers markers. Image allows quantification area individual fibers, nuclei location measurements, identification within each fiber. microscopy-based provides similar resolution contrast compared conventional techniques, robust multiplexed 3D reconstruction structures. These advantages make it valuable tool studying architecture, offering broad applications physiology pathology research. Key features • Enables capturing spatial relationships separation techniques. Supports detection multiple markers typing localization. Allows generation three-dimensional models analyze neuromuscular junctions, extracellular matrix, mitochondrial organization. Adaptable skeletal muscles species, providing insights into physiology, regeneration, disease processes. Graphical overview Analyzing composition morphology mice's microscopy. Workflow characterizing rodent Confocal microscopy, equipped with laser technology z-stack unmixing reduce through enhance detection. is beyond physical thickness sample account potential variations flatness ensure complete all focal planes. resulting images provide composition, areas, ensuring reproducible data analyzing characteristics other muscles.

Language: Английский

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Deficiency of ATF2 retards senescence induced by replication stress and pamidronate in mouse jaw bone marrow stem cells

Cellular Signalling, Journal Year: 2024, Volume and Issue: 127, P. 111579 - 111579

Published: Dec. 27, 2024

Language: Английский

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