Inflammation alters the expression and activity of the mechanosensitive ion channels in periodontal ligament cells DOI

Jianhan Ren,

Chaoning Zhan, Yifan Lin

et al.

European Journal of Orthodontics, Journal Year: 2024, Volume and Issue: 47(1)

Published: Dec. 4, 2024

Abstract Background Periodontal ligament cells (PDLCs) possess mechanotransduction capability, vital in orthodontic tooth movement (OTM) and maintaining periodontal homeostasis. The study aims to elucidate the expression profiles of mechanosensitive ion channel (MIC) families PDLCs how inflammatory mediator alters their function, advancing understanding biological process OTM. Methods methods Human were cultured exposed TNF-α. RNA sequencing was conducted explore mRNA transcriptome both normal TNF-α-treated PDLCs. Differentially expressed MICs identified analyzed. functional expressions TRPA1 TRPM8 further validated by RT-qPCR, Western blot, calcium influx assays. Results All 10 MIC or subfamilies PDLCs, with TRP family being most abundant. KCNK2, PIEZO1, TMEM87A, PKD2 channels TNF-α altered families, resulting increased PIEZO, K2P, TRP, TMEM63, TMEM87 decreased ENaC/ASIC, TMC/TMHS/TMIE, TMEM150, TMEM120, L/T/N-Type families. Furthermore, 17 DEMICs (false discovery rate < 0.05), top five (fold change ≥ 2), including upregulated TRPM8. verified, suggesting that significantly sensitized activities. Conclusions provides comprehensive reveals inflammation activities MICs. Treatments targeting these may offer promising strategies for improving OTM preventing complications environments, ultimately leading more effective safer practices.

Language: Английский

The effect of the Wnt pathway on the osteogenic differentiation of periodontal ligament stem cells in different environments DOI Creative Commons
Qi Su, F.‐M. Huang, Xin Fang

et al.

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18770 - e18770

Published: Jan. 3, 2025

Alveolar bone defects have always been an urgent problem in the oral cavity. For some patients with periodontal disease or undergoing orthodontic treatment implant restoration, alveolar can greatly inconvenience clinical diagnosis and treatment. Periodontal ligament stem cells (PDLSCs) are considered a promising source for cell therapy due to their high osteogenic differentiation capability. The of PDLSCs is influenced by various cytokines mediating signaling pathways, as well range biochemical, physical, chemical factors. Different environments typically impact Wnt pathway consequently affect PDLSCs. Understanding effects on contributes addressing diverse scenarios encountered environment. This knowledge aids influencing through bioengineering development novel materials, thereby enhancing potential providing new diagnostic therapeutic strategies practice.

Language: Английский

Citations

1
Hypoxia enhances osteoclastogenesis in periodontal ligament cells via expression of VEGF and RANKL DOI Creative Commons

Kenchi Hase,

Misa Ishiyama,

Shoko Ozawa

et al.

Pediatric Dental Journal, Journal Year: 2025, Volume and Issue: 35(1), P. 100336 - 100336

Published: Jan. 8, 2025

Language: Английский

Citations

0
Collaborative management of severe periodontal-endodontic lesion with intense lip sucking: a case report DOI Creative Commons
Yanfei Sun,

Xiaofang Zhao,

Mingchao Wang

et al.

BMC Oral Health, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 21, 2025

Lip behaviors can lead to maxillofacial developmental disorders such as anterior open bite, posterior crossbite, increased overjet, and a higher risk of developing Class II malocclusion. However, lip-sucking, behavior often underreported overlooked, may adversely affect the periodontal health permanent teeth in adolescents under extreme conditions if not promptly identified managed. We report unique case an 11-year-old boy with severe periodontal-endodontic lesion on tooth 32, caused by prolonged, high-frequency, high-intensity lip sucking. Initial clinical examination revealed gingival recession, Grade III mobility, deep pocket, alveolar bone loss without obvious pathogenesis. The therapeutic schedule encompassed behavioral modification, repeated application various dental splints due splint breakage strong lip-sucking habits, root canal therapy. condition stability were improved through collaborative management. At 18-month follow-up, improvements stability, density, reduced pocket depth observed. This is first exploring complex relationship between health, emphasizing that oral habits serious consequences. Timely identification intervention managing halt disease progression ensure favorable outcomes. Additionally, this offers treatment guidelines recommendations for diseases detrimental habits.

Language: Английский

Citations

0
Shear stress preconditioning enhances periodontal ligament cell survival DOI
Ravipha Suwittayarak, Nuttha Klincumhom,

Chaloemrit Phrueksotsai

et al.

Archives of Oral Biology, Journal Year: 2025, Volume and Issue: 173, P. 106232 - 106232

Published: March 11, 2025

Language: Английский

Citations

0
Construction of a deep learning-based predictive model to evaluate the influence of mechanical stretching stimuli on MMP-2 gene expression levels in fibroblasts DOI

Rihai Xiao,

Haowei Zhou,

Zhen Shi

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Abstract Background:Matrix metalloproteinase-2 (MMP-2) secretion homeostasis, governed by the multifaceted interplay of skin stretching, is a pivotal determinant influencing wound healing dynamics. This investigation endeavors to devise an artificial intelligence (AI) prediction framework delineating modulation MMP-2 expression under stretching conditions, thereby unravelling profound insights into mechanobiological orchestration MMP-2secretion and fostering novel mechanotherapeutic strategies targeted at modulation. Methods:Employing bespoke mechanical tensile loading apparatus, diverse stimuli were administered fibroblasts, with parameters such as shape frequency duration constituting regimen. Furthermore, reverse transcription polymerase chain reaction (RT‒PCR) assays conducted measure gene levels in fibroblasts subjected stretching. Subsequently, resulting data partitioned training validation cohorts 7:3 ratio, facilitating development deep learning (DL) model via back propagation neural network predicated on set. An external set was also curated culling pertinent literature from PubMed database assess predictive ability model. Results: Analysis 336 points related RT‒PCR corroborated variability response distinct regimens. Consequently, DL successfully crafted backpropagation algorithm delineate impact levels. The model, characterized R²value 0.73, evinced commendable fit dataset, elucidating intricate between input features target variable. Notably, exhibited minimal errors, evidenced root mean square error (RMSE) 0.42 absolute (MAE) 0.28. showcased robust generalization capabilities during validation, yielding R²values 0.70 0.71 for sets, respectively, revealing its accuracy. Conclusions: The fashioned through adeptly forecasts relative precision. These findings provide foundation MMP homeostasis expedite recalcitrant chronic refractory (CRW).

Language: Английский

Citations

0
Bioimplant-on-a-Chip for Facile Investigation of Periodontal Ligament Formation on Biogenic Hydroxyapatite/Ti6Al4 V Implants DOI Creative Commons
Sangbae Park,

Jae Eun Kim,

Juo Lee

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: May 13, 2025

Highly osseointegrative dental implants surrounded by reconstructed periodontal tissues represent a promising strategy for functional tooth replacement, as they mimic the structural and physiological characteristics of natural teeth. However, there is currently lack in vitro platforms that can effectively evaluate integration engineered ligament (PDL) with bioimplants. In this study, we developed bioimplant-on-a-chip (BoC) platform designed to recapitulate native PDL-cementum interface assess early stage biological performance bioimplants vitro. The BoC consists implant, calcium phosphate cement (CPC) insert, nanopatterned polydimethylsiloxane (PDMS) substrate, PDL-like tissue derived from human pulp stem cells (DPSCs). To establish viable culture conditions within platform, surface coatings cell seeding densities were optimized support formation tissue. Nanogrooved substrates incorporated guide cellular alignment, which was assessed through orientation analysis. Collagen fiber organization matrix deposition further examined indicators ligamentous maturation. Cementogenic activity evaluated immunofluorescent staining cementum protein-1 (CEMP-1) response varying biogenic hydroxyapatite (bHA) contents results demonstrated successful reproduction material-dependent differences CEMP-1 expression. This provides modular reproducible tool comparative evaluation physiologically relevant setting may be useful advancing regenerative strategies implantology.

Language: Английский

Citations

0
Effects of Stress on Biological Characteristics and Metabolism of Periodontal Ligament Stem Cells of Deciduous Teeth DOI Creative Commons
Z. Li, Jinyi Li, Shanshan Dai

et al.

International Dental Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Periodontal ligament stem cells (PDLSCs) from deciduous teeth (DePDLSCs) can perceive and respond to mechanical signals upon exposure various environments. The effects of stress on the biological characteristics metabolism DePDLSCs were investigated using in vitro loading.

Language: Английский

Citations

0
Predictive models for bone remodeling during orthodontic tooth movement: a scoping review on the “biological metamaterial” periodontal ligament interface DOI
Michele Tepedino, Francesco D’Annibale, Ivan Giorgio

et al.

Continuum Mechanics and Thermodynamics, Journal Year: 2024, Volume and Issue: 37(1)

Published: Nov. 16, 2024

Language: Английский

Citations

0
Inflammation alters the expression and activity of the mechanosensitive ion channels in periodontal ligament cells DOI

Jianhan Ren,

Chaoning Zhan, Yifan Lin

et al.

European Journal of Orthodontics, Journal Year: 2024, Volume and Issue: 47(1)

Published: Dec. 4, 2024

Abstract Background Periodontal ligament cells (PDLCs) possess mechanotransduction capability, vital in orthodontic tooth movement (OTM) and maintaining periodontal homeostasis. The study aims to elucidate the expression profiles of mechanosensitive ion channel (MIC) families PDLCs how inflammatory mediator alters their function, advancing understanding biological process OTM. Methods methods Human were cultured exposed TNF-α. RNA sequencing was conducted explore mRNA transcriptome both normal TNF-α-treated PDLCs. Differentially expressed MICs identified analyzed. functional expressions TRPA1 TRPM8 further validated by RT-qPCR, Western blot, calcium influx assays. Results All 10 MIC or subfamilies PDLCs, with TRP family being most abundant. KCNK2, PIEZO1, TMEM87A, PKD2 channels TNF-α altered families, resulting increased PIEZO, K2P, TRP, TMEM63, TMEM87 decreased ENaC/ASIC, TMC/TMHS/TMIE, TMEM150, TMEM120, L/T/N-Type families. Furthermore, 17 DEMICs (false discovery rate < 0.05), top five (fold change ≥ 2), including upregulated TRPM8. verified, suggesting that significantly sensitized activities. Conclusions provides comprehensive reveals inflammation activities MICs. Treatments targeting these may offer promising strategies for improving OTM preventing complications environments, ultimately leading more effective safer practices.

Language: Английский

Citations

0