On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology DOI Creative Commons
Na Su, Binbin Liu, Junru Wang

et al.

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 11, 2021

The recognition, identification, and differentiation of closely related plant species present significant notorious challenges to taxonomists. Maddenia group Prunus, which comprises four seven species, is an example a in delimitation phylogenetic reconstruction have been difficult, due the lack clear morphological distinctions, limited sampling, low informativeness molecular evidence. Thus, precise number relationships among them remain unclear. Here, we used genome skimming generate DNA sequence data for 22 samples, including 17 individuals five outgroups Amygdaloideae Rosaceae, from assembled plastome 446 single-copy nuclear (SCN) genes each sample. were then reconstructed using both concatenated coalescent-based methods. We also identified eight highly variable regions detected simple repeats (SSRs) repeat sequences plastomes. analysis based on complete plastomes strongly supported three main subclades while recognized tree. network six hybridization events. Integrating evidence, proposed recognize within group, i.e., Prunus fujianensis, P. himalayana, gongshanensis, hypoleuca, hypoxantha. Within this first are well-supported, gene flow occurring throughout seems be especially frequent between hypoleuca hypoxantha, eroding barrier them. trees hypervariable had similar topology with plastomes, showing their potential as markers effective barcodes further phylogeographic studies Maddenia.

Language: Английский

Comparative and Phylogenetic Analysis of the Chloroplast Genomes of Four Wild Species of the Genus Prunus DOI Open Access

Max Cui,

Chenxi Liu, Xiumei Yang

et al.

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 239 - 239

Published: Feb. 20, 2025

Background: Prunus armeniaca, divaricata, tianshanica, and domestica are valuable ancient tree species that have persisted since the end of Tertiary period within Tianshan wild fruit forest. However, evolutionary relationships among in forest long posed a challenge. Methods: We sequenced assembled chloroplast genomes P. incorporated genome data for comparative analysis to elucidate their phylogenetic positions genus Prunus. Results: The lengths these ranged from 157,395 bp 158,090 bp, with total 130 131 genes annotated, comprising 85 86 protein-coding genes, 8 rRNA 35 36 tRNA genes. Notably, four predominantly used high-frequency codons ending A/U. Additionally, we identified 300 simple repetitive sequences 166 across species. mutation sites were mainly found non-coding regions, seven regions high frequency identified. revealed five branches: subgenus Armeniaca, Microcerasus, Prunus, Amygdalus, Cerasus. estimated deviation time crown group is roughly 61.41 million years ago. Conclusions: This study provides exhaustive genetic evidence classification systematic establishes crucial foundation subsequent research into diversity history genus.

Language: Английский

Citations

0
On the Species Delimitation of the Maddenia Group of Prunus (Rosaceae): Evidence From Plastome and Nuclear Sequences and Morphology DOI Creative Commons
Na Su, Binbin Liu, Junru Wang

et al.

Frontiers in Plant Science, Journal Year: 2021, Volume and Issue: 12

Published: Oct. 11, 2021

The recognition, identification, and differentiation of closely related plant species present significant notorious challenges to taxonomists. Maddenia group Prunus, which comprises four seven species, is an example a in delimitation phylogenetic reconstruction have been difficult, due the lack clear morphological distinctions, limited sampling, low informativeness molecular evidence. Thus, precise number relationships among them remain unclear. Here, we used genome skimming generate DNA sequence data for 22 samples, including 17 individuals five outgroups Amygdaloideae Rosaceae, from assembled plastome 446 single-copy nuclear (SCN) genes each sample. were then reconstructed using both concatenated coalescent-based methods. We also identified eight highly variable regions detected simple repeats (SSRs) repeat sequences plastomes. analysis based on complete plastomes strongly supported three main subclades while recognized tree. network six hybridization events. Integrating evidence, proposed recognize within group, i.e., Prunus fujianensis, P. himalayana, gongshanensis, hypoleuca, hypoxantha. Within this first are well-supported, gene flow occurring throughout seems be especially frequent between hypoleuca hypoxantha, eroding barrier them. trees hypervariable had similar topology with plastomes, showing their potential as markers effective barcodes further phylogeographic studies Maddenia.

Language: Английский

Citations

21