The diagnostic relevance of mesenteric lymph node biopsy in small intestinal lymphoma in cats

Journal of Veterinary Internal Medicine, Journal Year: 2024, Volume and Issue: 38(4), P. 2316 - 2323

Published: June 10, 2024

Abstract Background Regional lymph nodes are frequently sampled in cats with suspected intestinal lymphoma; however, their diagnostic value has not been explored. Objectives To investigate whether histologic and immunohistochemical analysis of mesenteric correlates the diagnosis lymphoma cats. Animals One hundred 2 client‐owned diagnosed lymphoma. Methods Retrospective study. The inclusion criteria required a full‐thickness biopsy small intestine concurrent excision nodes. Histologic immunophenotypic analyses were performed on biopsies corresponding Selected nodal samples reactive underwent clonality testing. Results Transmural T‐cell lymphomas, encompassing large cell types, predominant (64 cases, 62.7%), B‐cell lymphomas being more transmural (68.8%) than mucosal (31.2%). Among all examined, 44 (43.1%; 95% CI: 33.9%‐52.8%) exhibited neoplastic infiltration. cases lymphoma, 51 out 72 (70.8%; 59.4%‐80.1%) showed no involvement. Clonality results correctly identified 19/30 (63.3%; 45.5%‐78.2%) Concerns raised regarding clonal identification remaining potential misdiagnoses based phenotypic characteristics. Conclusion Clinical Importance study underscores drawbacks relying solely for diagnosing cats, particularly subtypes. It emphasizes importance assessing infiltration recommends caution when utilizing histologic, evaluations lymphomas. Despite limitations, this research highlights need comprehensive strategies

Language: Английский

Morphological, phenotypical and molecular characterization of canine lymphomas with dual T- and B-cell markers expression

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: April 22, 2025

Recent investigations have identified rare, phenotypically complex lymphoma variants, including cases exhibiting concurrent expression of T- and B-cell markers. These atypical presentations suggest perturbations in lymphoid differentiation programs or clonal evolution, necessitating more sophisticated diagnostic approaches. The CD3 CD20 canine lymphomas represents a particularly compelling phenomenon documented across various anatomical sites. Molecular diagnostics, PCR for Antigen Receptor Rearrangements (PARR), provided crucial insights into these cases, revealing rearrangements both TCRγ IgH selected further challenging traditional classification paradigms. Here, we report 33 double-positive lymphoma, retrieved with retrospective analysis the MyLAV Diagnostic Laboratory electronic database. Specifically, results an integrated approach combining WHO-based morphological classification, comprehensive immunohistochemical immunophenotyping T-cell (CD3 CD5) markers (CD20 PAX5), PARR analysis. skin, oral/nasal mucosa mucocutaneous junction were most commonly affected sites, accounting 24 (72.7%). All stained positive (100%), 32 (97%) CD5, only 12 (36.4%) PAX5. Aberrant cytoplasmic localization was found 29 (87.9%) cases. revealed rearrangement signals TCR gene 23 (69.7%) CBmajor CBminor 9 (27.3%). findings emphasize that while immunohistochemistry remains fundamental tool, it may be insufficient isolation definitive lineage determination emerges as essential complementary technique distinguishing between aberrant marker true biphenotypic differentiation.

Language: Английский

Histologic, Phenotypic, and Molecular Characterization of Feline Hodgkin‐Like Lymphoma With Classical Reed‐Sternberg Cells

Veterinary and Comparative Oncology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

ABSTRACT Hodgkin‐like lymphoma (HLL) is a rare neoplasm in cats that shares characteristics with the human disease. The hallmark of HLL presence Reed‐Sternberg (RS) cells expressing CD30 and CD20. This study aimed to elucidate clinicopathologic features, immunophenotype clonality patterns feline HLL. A comprehensive retrospective review molecular data nodal lymphomas over 6‐year period was conducted MyLav laboratory. Twenty‐four cases were identified. All presented submandibular or retropharyngeal lymphadenopathy. Histopathologic examination revealed multifocal diffuse proliferation medium‐to‐large lymphoid low mitotic activity, interspersed RS cells, heterogeneous inflammatory infiltrate comprising T‐cells, plasma neutrophils. In addition, extensive necrosis consistent finding. Immunohistochemistry showed variable membranous CD20 nuclear PAX5 expression neoplastic while displayed only mild moderate positivity negative PAX5. 21/24 (87.5%), diffusely CD30‐positive. PARR analysis demonstrated clonal B‐cell expansion 60% cases, remaining 40% exhibiting polyclonality. For 10 available follow‐up, prognosis generally favourable, two succumbing progressive conclusion, diagnosing challenging. by should be considered disease, but after excluding differential diagnoses such as anaplastic granulomatous

Language: Английский