A sea of worms: the striking cases of the European Perinereis cultrifera and P. rullieri (Annelida: Nereididae) species complexes, with description of 13 new species

Invertebrate Systematics, Journal Year: 2025, Volume and Issue: 39(2)

Published: Feb. 20, 2025

Molecular data have been suggesting the existence of a complex cryptic species within taxon Perinereis cultrifera , which has not fully explored yet. In this study, we performed morphological and molecular analysis (mtCOI-5P 16S rRNA 28SD2 ) specimens from intertidal marine brackish European localities, mostly focusing on Mediterranean Sea Canary Islands. Two major phylogenetic clades with at least 18 divergent (COI 19.8; 6.4-28.5%) completely sorted lineages were uncovered based original data, 13 occurred exclusively in Sea, further 5 are unique to Italian waters. An additional morphologically similar lineage, corresponding P. oliveirae coexisting single NE Atlantic lineage complex, was also retrieved as an ingroup. Careful inspection, combined deep divergence between two perfect match each clade specific paragnath chaetal types, highlighted distinct groups species: Clade A, shows features matching historical descriptions B overlooked morphotype described rullieri . Although paragnaths show pattern clades, their sizes considerably smaller chaetae characterised by coarse serration base spiniger blades long falciger blades, opposed lightly serrated short falcigers Further mainly thickness, direction length paragnaths, well expansion posteriormost dorsal ligules revealed clade, together geographic environmental boundaries allowed for differentiation most these without data. Thirteen new here formally described, eight belonging A: caesarea sp. nov., faulwetterae houbinae maleniae miquellai muscoi nieri nov. twobae nov.; five B: castellii juno jupiter minerva tibicena The combination beaucoudrayi is proposed Nereis previously considered synonymous only occurring Atlantic. Lastly, s.s., s.s ingroup redescribed using topotypical material, available syntypes lectotypes assigned former two. ZooBank: urn:lsid:zoobank.org:pub:28C64123-DE82-411D-BC96-5E892FC692E3.

Language: Английский

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Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia

Regional Studies in Marine Science, Journal Year: 2020, Volume and Issue: 36, P. 101307 - 101307

Published: April 1, 2020

DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy species-level assignment, consequent taxonomic coverage, relies on comprehensive barcode reference libraries, which, due to incompleteness, are currently recognized limitation for applications. In this study, we assembled library barcodes Atlantic Iberia marine species, assessed gaps in species coverage examined data ambiguities. Initially, an Iberian checklist the three dominant groups macroinvertebrates was compiled, comprising 2827 (926 Annelida, 638 Crustacea 1263 Mollusca). A total 18162 sequences cytochrome c oxidase subunit I region (COI-5P) matching were compiled BOLD dataset, where discordances evaluated cases deep intraspecific divergence flagged. Gap-analysis showed that 63% still lack barcode. Coverage varied considerably across with Mollusca displaying highest sequence representation dataset (427 49% number sequences), 338 barcoded (53% checklist). contrast, Polychaeta displayed lower levels completion (288 16% sequences). total, 1545 Barcode Index Numbers (BINs) assigned 1053 which 66% taxonomically concordant, 26% multiple BINs 8% discordant. Overall, results show there is large portion invertebrate taxa Europe pending even considering only groups. However, most notable finding relevant proportion flagged significant possible hidden diversity. annotated gap-analysis here provided can therefore contribute prioritize future research efforts coverage.

Language: Английский

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On the Diversity of Phyllodocida (Annelida: Errantia), with a Focus on Glyceridae, Goniadidae, Nephtyidae, Polynoidae, Sphaerodoridae, Syllidae, and the Holoplanktonic Families

Diversity, Journal Year: 2021, Volume and Issue: 13(3), P. 131 - 131

Published: March 17, 2021

Phyllodocida is a clade of errantiate annelids characterized by having ventral sensory palps, anterior enlarged cirri, axial muscular proboscis, compound chaetae (if present) with single ligament, and lacking dorsolateral folds. Members most families date back to the Carboniferous, although earliest fossil was dated from Devonian. holds 27 well-established morphologically homogenous clades ranked as families, gathering more than 4600 currently accepted nominal species. Among them, Syllidae Polynoidae are specious polychaete groups. Species mainly found in marine benthos, few inhabit freshwater, terrestrial planktonic environments, occur intertidal deep waters all oceans. In this review, we (1) explore current knowledge on species diversity trends (based traditional concept molecular data), phylogeny, ecology, geographic distribution for whole group, (2) try identify main gaps, (3) focus selected families: Alciopidae, Goniadidae, Glyceridae, Iospilidae, Lopadorrhynchidae, Polynoidae, Pontodoridae, Nephtyidae, Sphaerodoridae, Syllidae, Tomopteridae, Typhloscolecidae, Yndolaciidae. The highest richness concentrated European, North American, Australian continental shelves (reflecting strong sampling bias). While data come shallow coastal surface environments world oceans clearly under-studied. overall indicate that new descriptions constantly added through time less 10% known have barcode information available.

Language: Английский

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On Anguillosyllis cf. hessleri Maciolek, 2020 – A species complex from the Clarion-Clipperton zone, abyssal central Pacific

Deep Sea Research Part I Oceanographic Research Papers, Journal Year: 2025, Volume and Issue: 220, P. 104453 - 104453

Published: April 4, 2025

Language: Английский

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Revealing the diversity of the green Eulalia (Annelida, Phyllodocidae) species complex along the European coast, with description of three new species

Organisms Diversity & Evolution, Journal Year: 2023, Volume and Issue: 23(3), P. 477 - 503

Published: Jan. 13, 2023

Language: Английский

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Reappraisal of the hyperdiverse

Invertebrate Systematics, Journal Year: 2022, Volume and Issue: 36(11), P. 1017 - 1061

Published: Nov. 16, 2022

Morphologically similar species are often overlooked but molecular techniques have been effective in signalling potential hidden diversity, boosting the documentation of unique evolutionary lineages and ecological diversity. Platynereis dumerilii massiliensis part a recognised complex, where differences reproductive biology mainly highlighted to date. Analyses DNA sequence data (COI, 16S rDNA D2 region 28S rDNA) populations apparent morphotype P. obtained from broader sampling area along European marine waters, including Azores Webbnesia islands (Madeira Canaries), provided compelling evidence for existence at least 10 divergent lineages. Complementing genetic data, morphological observations better represented revealed two major groups with distinctive paragnath patterns. Two new were erected: nunezi sp. nov., widespread islands, jourdei restricted western Mediterranean. The combination agilis is also proposed Nereis agilis, previously unaccepted one present both Northeast Atlantic redescribed based on topotypic material. However, uncertainty identity due original brief description absence type material prevents unequivocal assignment lineage assumed this previous studies. remaining five by only few small specimens features poorly preserved therefore not described study. ZooBank: urn:lsid:zoobank.org:pub:50079615-85E5-447E-BDD7-21E81C2A6F4D

Language: Английский

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Integrative taxonomy in Syllis prolifera (Annelida, Syllidae): from a unique cosmopolitan species to a complex of pseudocryptic species

Invertebrate Systematics, Journal Year: 2024, Volume and Issue: 38(6)

Published: June 19, 2024

Syllis prolifera (Syllidae, Syllinae) is an abundant species of marine annelids commonly found in warm to temperate waters worldwide. Although morphological variability occurs among populations, S. has long been considered a cosmopolitan species, widely distributed coastal environments, including acidified and polluted areas. However, the increasing number cases cryptic pseudocryptic speciation several polychaete families recent years led us question whether represents single globally taxon or complex. To address this question, we conducted integrative study, combining morphological, ecological molecular data 52 specimens collected different localities across western Mediterranean Sea Gulf Cadiz. Our phylogenetic delimitation analyses that included two mitochondrial DNA markers (COI 16S rRNA) were congruent not considering unique entity. Five distinct lineages can also be recognised by certain traits identified from these instead. Overall, our study does support homogeneity Sea, providing new example pseudocrypticism invertebrates.

Language: Английский

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Molecular diversity within the genus Laeonereis (Annelida, Nereididae) along the west Atlantic coast: paving the way for integrative taxonomy

PeerJ, Journal Year: 2021, Volume and Issue: 9, P. e11364 - e11364

Published: May 27, 2021

The polychaete genus Laeonereis (Annelida, Nereididae) occurs over a broad geographic range and extends nearly across the entire Atlantic coast of America, from USA to Uruguay. Despite research efforts clarify its diversity systematics, mostly by morphological ecological evidence, there is still uncertainty, mainly concerning species culveri , which constitutes an old notorious case taxonomic ambiguity. Here, we revised molecular distribution based on multi-locus approach, including DNA sequence analyses partial segments cytochrome c oxidase subunit I (COI), 16S rRNA, 28S rRNA genes. We examined specimens collected 26 sites along American Massachusetts (USA) Mar del Plata (Argentina). Although no comprehensive examination was performed between different populations, COI barcodes revealed seven highly divergent MOTUs, with mean K2P genetic distance 16.9% (from 6.8% 21.9%), confirmed through four clustering algorithms. All MOTUs were geographically segregated, except for 6 7 southeastern Brazil, presented partially overlapping ranges Rio de Janeiro São Paulo coast. Sequence data obtained markers supported same MOTU delimitation segregation as those COI, providing further evidence existence deeply lineages within genus. extent divergence observed in our study fits comfortably reported polychaetes, Nereididae, thus strong indication that they might constitute separate species. These results may therefore pave way integrative studies, aiming status herein reported.

Language: Английский

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Polychaetes (Annelida) of Cyprus (Eastern Mediterranean Sea): An Updated and Annotated Checklist including New Distribution Records

Diversity, Journal Year: 2023, Volume and Issue: 15(8), P. 941 - 941

Published: Aug. 19, 2023

The diversity and distribution of polychaetes in the coastal area EEZ Republic Cyprus is presented based on both literature records new data acquired a wide range environmental monitoring programmes research projects. A total 585 polychaete species belonging to 49 families were reported waters; among them, 205 (34%) recorded only, 149 (26%) our own data, 231 spp. (40%) from data. 51 identified as non-indigenous; 32 confirmed alien species, 4 considered cryptogenic, 15 questionable there doubts about their identity. Indo-Pacific Schistomeringos loveni was for first time Mediterranean Sea, while four already literature, namely, Bispira melanostigma, Fimbriosthenelais longipinnis Leonnates aylaoberi, Rhodopsis pusilla, added list non-indigenous Sea. current work highlights importance implementing carrying out surveys targeting benthic macrofauna assemblages.

Language: Английский

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From 13 to 22 in a second stroke: revisiting the European Eumida sanguinea (Phyllodocidae: Annelida) species complex

Zoological Journal of the Linnean Society, Journal Year: 2021, Volume and Issue: 196(1), P. 169 - 197

Published: Oct. 16, 2021

Abstract Eumida sanguinea is a recognized polychaete species complex which, in previous studies, has been reported to have additional undescribed diversity. We detected nine lineages by analysing DNA sequence data (mitochondrial: COI, 16S rRNA and nuclear loci: ITS region 28S rRNA) of E. morphotype populations from broader sampling effort European marine waters. Traditional morphological features failed provide consistent differences or unique characters that could be used distinguish these species. However, complementing with morphometrics, geographic range, colour pigmentation patterns, we revealed five new Two derived the previously signalled S21 GB22, which are here named as schanderi sp. nov. fenwicki nov., respectively. Three other based on newly discovered lineages, namely fauchaldi langenecki pleijeli From six remaining, three represented fewer than two well-preserved specimens, prevented further comprehensive analysis. The last were only distinct when using mitochondrial markers. Integrative taxonomy essential elucidate evolutionary phenomena eventually allow informed use complexes exhibiting stasis biomonitoring ecological studies.

Language: Английский

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