Unearthing soil arthropod diversity through DNA metabarcoding

PeerJ, Journal Year: 2022, Volume and Issue: 10, P. e12845 - e12845

Published: Feb. 1, 2022

DNA metabarcoding has the potential to greatly advance understanding of soil biodiversity, but this approach seen limited application for most abundant and species-rich group fauna–the arthropods. This study begins address gap by comparing information on species composition recovered from two types bulk samples (specimens, soil) a temperate zone site collected at eight sites in Arctic. Analysis 22 (3 specimen, 19 revealed 410 arthropod OTUs belonging 112 families, 25 orders, nine classes. Studies little overlap between specimen samples, more higher taxonomic levels (families, orders) congruent patterns α - β -diversity. Expansion analyses Arctic locally rich, highly dissimilar, spatially structured assemblages compatible with dispersal environmentally driven assembly. The current demonstrates that enables rapid, large-scale assessments diversity. However, deep sequence coverage is required adequately capture present these expansion barcode reference library necessary improve resolution sequences through approach.

Language: Английский

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Tree diversity enhances predation by birds but not by arthropods across climate gradients

Ecology Letters, Journal Year: 2024, Volume and Issue: 27(5)

Published: May 1, 2024

Abstract Tree diversity can promote both predator abundance and diversity. However, whether this translates into increased predation top‐down control of herbivores across taxonomic groups contrasting environmental conditions remains unresolved. We used a global network tree experiments ( TreeDivNet ) spread three continents biomes to test the effects species richness on varying climatic temperature precipitation. recorded bird arthropod attempts plasticine caterpillars in monocultures mixtures. Both by birds but not arthropods. Furthermore, were consistent studied gradient. Our findings provide evidence that strengthens insect birds, underscoring need implement conservation strategies safeguard sustain ecosystem services provided natural enemies forests.

Language: Английский

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Data of the Insect Biome Atlas: a metabarcoding survey of the terrestrial arthropods of Sweden and Madagascar

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract We present the data from Insect Biome Atlas project (IBA), characterizing terrestrial arthropod faunas of Sweden and Madagascar. Over 12 months, weekly Malaise trap samples were collected at 203 locations within 100 sites in 50 33 Madagascar; this was complemented by soil litter each site. The field comprise 4,749 trap, 192 2,566 190 Samples processed using mild lysis or homogenization, followed DNA metabarcoding COI (418 bp). 698,378 non-chimeric sequence variants 687,866 Madagascar, representing 33,989 (33,046 Arthropoda) 77,599 (77,380 operational taxonomic units, respectively. These are most comprehensive presented on these so far, allowing unique analyses size, composition, spatial turnover seasonal dynamics sampled communities. They also provide an invaluable baseline against which to gauge future changes.

Language: Английский

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Long-term archival of environmental samples empowers biodiversity monitoring and ecological research

Environmental Sciences Europe, Journal Year: 2022, Volume and Issue: 34(1)

Published: May 10, 2022

Abstract Human-induced biodiversity loss and changes in community composition are major challenges of the present time, urgently calling for comprehensive biomonitoring approaches to understand system dynamics inform policy-making. In this regard, molecular methods increasingly applied. They provide tools fast high-resolution assessments can also focus on population or functional diversity. If samples stored under appropriate conditions, will enable analysis DNA, but RNA proteins from tissue non-biological substrates such as soil, water, sediments, so-called environmental DNA (eDNA) eRNA. Until now, most studies using rely recent sampling events, although benefit analyzing long-time series is obvious. context Environmental Specimen Banks (ESBs) play a crucial role, supplying diverse well-documented collected periodically repeated following standardized protocols. Mainly assembled integrative monitoring chemical compounds, ESB collections largely accessible third parties principle be used analysis. While ESBs hold great potential storage samples, cooperation with Biodiversity Biobanks scientific guarantees nucleotide (DNA, RNA) extracts together links analytical results metadata. The contribution aims raise awareness research regarding high-quality through ESBs, encourages collect store DNA-friendly ways, points out high combining DNA-based chemicals other stressors.

Language: Английский

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Community assembly and metaphylogeography of soil biodiversity: Insights from haplotype‐level communityDNAmetabarcoding within an oceanic island

Molecular Ecology, Journal Year: 2022, Volume and Issue: 31(15), P. 4078 - 4094

Published: June 4, 2022

Most of our understanding island diversity comes from the study aboveground systems, while patterns and processes diversification community assembly for belowground biotas remain poorly understood. Here, we take advantage a relatively young dynamic oceanic to advance ecoevolutionary driving within soil mesofauna. Using whole organism DNA (wocDNA) metabarcoding recently developed metaMATE pipeline, have generated spatially explicit reliable haplotype-level sequence data mesofaunal assemblages sampled across four main habitats Tenerife. Community ecological metaphylogeographic analyses been performed at multiple levels genetic similarity, haplotypes species supraspecific groupings. Broadly consistent local-scale richness different insular found, whereas local is lower than in continental settings. Our results reveal an important role niche conservatism as driver mesofauna, with only limited evidence habitat shifts promoting diversification. Furthermore, support found fundamental where specialism mainly due colonization establishment preadapted species. Hierarchical distance decay level metaphylogeographical pattern geographic structuring over spatial scales, through lineages, expected taxa strong dispersal limitations. demonstrate potential wocDNA biodiversity.

Language: Английский

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