Ecological indicators based on quantitative eDNA metabarcoding: the case of marine reserves

Ecological Indicators, Journal Year: 2022, Volume and Issue: 140, P. 108966 - 108966

Published: May 26, 2022

In a context of marine biodiversity erosion, the need to better understand effects overfishing stands out. New genetic techniques such as environmental DNA (eDNA) metabarcoding have emerged and allow detection wider range species compared conventional methods, but still fall short providing reliable abundance estimations subsequent ecological indicators. this paper, we propose combination quantitative polymerase chain reaction obtain quantity eDNA molecules per species. This method was used inside outside six no-take Mediterranean reserves measure effect protection on fish build new indicator. Even if total not different between reserves, detected that cryptobenthic significantly associated reserves. Based observation, novel indicator, Demerso-pelagic Benthic Ratio (DeBRa), taking advantage capacity detect reef fishes which are often missed by classical surveys. The DeBRa higher reflecting relative belonging pelagic demersal under against fishing, therefore it appears be eDNA-based indicator human pressure. Furthermore, sensitive habitat or variations does require complete reference database sequences since can rely assigned at genus family scale possible necessary.

Language: Английский

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Environmental DNA metabarcoding reveals the presence of invasive and cryptic species in the Mweru-Luapula fishery

Scientific African, Journal Year: 2025, Volume and Issue: unknown, P. e02544 - e02544

Published: Jan. 1, 2025

Language: Английский

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eDNA Metabarcoding Applications Across Italian Marine Coastal Ecosystems: An Overview

Environmental DNA, Journal Year: 2025, Volume and Issue: 7(2)

Published: March 1, 2025

ABSTRACT Climate emergency and other anthropogenic pressures urgently call for technological methodological advances to enhance our ability protect marine habitats their ecosystem services. In recent years, environmental DNA (eDNA) metabarcoding has emerged as a powerful tool achieve an integrative assessment of the health status, through broad, relatively rapid, cost‐effective taxonomic monitoring biodiversity at different spatial scales. Here we provide time‐based overview applications eDNA methodology carried out across diverse Italian coastal habitats, with in‐depth scrutiny commonly adopted operative procedures, from sampling sequencing. The lack standardization low replicability in space/time arose major issues several campaigns, preventing appropriate cross‐comparability previous studies. Given wide potential surveys along coastline, this review aims boost wider application inventories avoid weaknesses that could compromise long‐term value broad scope future plans.

Language: Английский

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Evaluation of Mediterranean sponges as natural samplers for environmental DNA (eDNA)

Open Research Europe, Journal Year: 2025, Volume and Issue: 5, P. 69 - 69

Published: March 17, 2025

Marine sponges have emerged as effective natural samplers of environmental DNA (eDNA), offering a promising alternative for biodiversity monitoring. By filtering large volumes seawater, accumulate eDNA from surrounding communities, potentially enhancing species detection in marine environments where conventional water sampling is limited. In this study, we evaluated the recovery efficiency seven Mediterranean sponge to identify optimal candidates biomonitoring. Axinella verrucosa outperformed other tested species, highlighting its potential long-term assessments. Our results align with previous findings that low microbial abundance (LMA) recover more than high (HMA) reinforcing need targeted selection future studies. Detected fish taxa were all bottom dwelling, supporting relevance monitoring cryptic and benthic habitats. As eDNA-based advances, offers valuable complement surveys, particularly habitats challenging.

Language: Английский

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Metabarcoding identifies macroalgal composition as a driver of benthic invertebrate assemblages in restored habitats

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 21, 2025

Abstract Large brown macroalgal forests support diverse communities of associated invertebrates. However, human activities have led to their degradation, replacing the original assemblages with less-complex habitats and altering invertebrate communities. To counteract this, restoration actions are currently being performed aiming at recovery. Here, we used metabarcoding COI gene describe community a 10-year restored forest dominated by Gongolaria barbata . This assemblage was compared an adjacent site where G. is absent, two healthy also , as references. Results showed that arthropods, molluscs, annelids were most abundant groups. Specific MOTUs representing sponge ascidian species exclusively found in assemblages, suggesting particularly benefited some these organisms. Crustaceans however, similarly across including one photophilous macroalgae (without ), thus indicating mobile groups may equally colonise alternative habitats. The overall composition did not completely resemble references, recovering all diversity complex process, likely influenced multiple factors require further investigation.

Language: Английский

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