Neopolyploidy increases stress tolerance and reduces fitness plasticity across multiple urban pollutants: support for the “general-purpose” genotype hypothesis

Evolution Letters, Journal Year: 2024, Volume and Issue: 8(3), P. 416 - 426

Published: Jan. 10, 2024

Abstract Whole-genome duplication is a common macromutation with extensive impacts on gene expression, cellular function, and whole-organism phenotype. As result, it has been proposed that polyploids have “general-purpose” genotypes perform better than their diploid progenitors under stressful conditions. Here, we test this hypothesis in the context of stresses presented by anthropogenic pollutants. Specifically, tested how multiple neotetraploid genetic lineages mostly asexually reproducing greater duckweed (Spirodela polyrhiza) across favorable control environment 5 urban pollutants (iron, salt, manganese, copper, aluminum). By quantifying population growth rate over generations, found most pollutants, but not all, polyploidy decreased actively growing propagules increased dormant ones. Yet, when considering total propagule production, tolerance to maintained population-level fitness diploids. Furthermore, broad-sense correlations among were all positive neopolyploids so for Our results provide rare support are more tolerant conditions can maintain diploids heterogeneous stresses. These may help predict be likely persist environments, such as those caused urbanization other human activities.

Language: Английский

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Neopolyploidy has variable effects on the diversity and composition of the wild strawberry microbiome

American Journal of Botany, Journal Year: 2024, Volume and Issue: 111(8)

Published: Feb. 17, 2024

Whole-genome duplication (neopolyploidy) can instantly differentiate the phenotype of neopolyploids from their diploid progenitors. These phenotypic shifts in organs such as roots and leaves could also way interact with microbial species. While some studies have addressed how specific interactions are affected by neopolyploidy, we lack an understanding genome affects diversity composition communities.

Language: Английский

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Neopolyploidy‐induced changes in giant duckweed (Spirodela polyrhiza) alter herbivore preference and performance and plant population performance

American Journal of Botany, Journal Year: 2024, Volume and Issue: 111(8)

Published: March 11, 2024

Abstract Premise Polyploidy is a widespread mutational process in angiosperms that may alter population performance of not only plants but also their interacting species. Yet, knowledge whether polyploidy affects plant–herbivore dynamics scarce. Here, we tested aphid herbivores exhibit preference for diploid or neopolyploid plants, impacts plant and herbivore performance, these interactions depend on the genetic background. Methods Using independently synthesized neotetraploid strains paired with progenitors greater duckweed ( Spirodela polyrhiza ), evaluated effect neopolyploidy duckweed's interaction water‐lily Rhopalosiphum nymphaeae ). paired‐choice experiments, feeding herbivore. We then consequences by measuring growth over multiple generations. Results Aphids preferred neopolyploids when were provided at equal abundances surface areas, suggesting role area driving this preference. Additionally, increased result was dependent plant's lineage. Lastly, impact herbivory vs. varied greatly lineage, where appeared to be variably tolerant compared diploids, sometimes mirroring performance. Conclusions By experimentally testing trophic species interactions, showed polyploidization can hosts. These results have significant implications establishment persistence face polyploidy.

Language: Английский

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Special issue.: The role of whole genome duplication in evolutionary ecology

Oikos, Journal Year: 2024, Volume and Issue: 2024(5)

Published: April 30, 2024

Polyploid organisms are common and can be found across the tree of life. A key question is to understand how why these polyploid lineages become established persist in populations, particularly since they predicted have a low probability success. While collection papers this special issue addresses broad questions on evolutionary ecology polyploids, ultimately, studies also highlight myriad ways that we examining what drives success lineages. In paper consider where we've been challenges face, then propose several directions will allow us continue propel field towards our ultimate goal understanding rules govern establishment persistence populations. We conclude developing rule set require combination model systems for which detailed knowledge phylogenetic population genetic history, expanding perspective beyond plants include greater taxonomic breadth, conducting ecologically relevant settings. Additionally, argue future research polyploidy should focus integrating theory empirical research, providing mechanistic linkages between effects whole genome duplication demography, build predictive framework anthropogenic change impact organisms.

Language: Английский

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Doubling down on polyploid discoveries: Global advances in genomics and ecological impacts of polyploidy

American Journal of Botany, Journal Year: 2024, Volume and Issue: 111(8)

Published: Aug. 1, 2024

Abstract All flowering plants are now recognized as diploidized paleopolyploids (Jiao et al., 2011; One Thousand Plant Transcriptomes Initiative, 2019), and polyploid species comprise approximately 30% of contemporary plant (Wood 2009; Barker 2016a). A major implication these discoveries is that, to appreciate the evolution diversity, we need understand fundamental biology polyploids diploidization. This broadly by our community there a continued, growing interest in polyploidy research topic. Over past 25 years, sequencing analysis genomes has revolutionized understanding importance speciation land plants.

Language: Английский

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The immediate metabolomic effects of whole‐genome duplication in the greater duckweed, Spirodela polyrhiza

American Journal of Botany, Journal Year: 2024, Volume and Issue: 111(8)

Published: Aug. 1, 2024

Abstract Premise In plants, whole‐genome duplication (WGD) is a common mutation with profound evolutionary potential. Given the costs associated superfluous genome copy, polyploid establishment enigmatic. However, in right environment, immediate phenotypic changes following WGD can facilitate establishment. Metabolite abundances are direct output of cell's regulatory network and determine much impact environmental genetic change on phenotype. While it well known that an increase bulk amount material cell size, gene dosage multiplication metabolome remains largely unknown. Methods We used untargeted metabolomics four genetically distinct diploid‐neoautotetraploid pairs greater duckweed, Spirodela polyrhiza , to investigate how affects metabolite per biomass. Results Autopolyploidy increased levels cell, but response individual metabolites varied considerably. level biomass was restricted because size reduced concentration cell. Nevertheless, we detected both quantitative qualitative effects metabolome. Many were strain‐specific, some shared by all strains. Conclusions The nature metabolic after depended strongly genotype. Dosage have potential alter plant qualitatively quantitatively, balanced out reduction due this species.

Language: Английский

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Multi-Omics Analyses Uncover the Mechanism Underlying Polyploidization-Enhanced Steviol Glycosides Biosynthesis in Stevia rebaudiana

Plants, Journal Year: 2024, Volume and Issue: 13(18), P. 2542 - 2542

Published: Sept. 10, 2024

Stevia rebaudiana (Bertoni) is a valuable sweetener plant whose sweetness primarily derives from steviol glycosides (SGs), especially rebaudioside A (RA). Polyploidization has the potential to enhance content of active ingredients in medicinal plants, making this strategy promising avenue for genetic improvement. However, underlying regulatory mechanisms that contribute fluctuating SGs between autotetraploid and diploid stevia remain unclear. In study, we employed metabolic analysis identify 916 differentially accumulated metabolites (DAMs), with majority, specifically terpenoids, flavonoids, lipids, exhibiting upregulation due polyploidization. Notably, stevia’s signature metabolite (including RA, steviolbioside, C), along their precursor steviol, increased significantly after Furthermore, comprehensive transcriptome metabolome revealed majority expressed genes (DEGs) involved SG-synthesis pathway (ent-KAH, ent-KS1, UGT73E1, UGT74G1, UGT76G1, UGT85C2, UGT91D2) were upregulated stevia, these DEGs exhibited positive correlation polyploidization-enhanced SGs. Additionally, multi-omics network indicated several transcription factor families (such as five NACs, four WRKYs, three MYBs, eight bHLHs, AP2/ERFs), various transporter (four ABC transporters, triose-phosphate two sugar efflux transporters intercellular exchange), well microorganisms Ceratobasidium Flavobacterium) positively correlated accumulation RA steviol. Overall, our results indicate presence circuit orchestrated by polyploidization, which recruits beneficial rhizosphere microbes modulates expression associated SG biosynthesis, ultimately enhancing stevia. This finding will provide new insights promoting propagation industrial development

Language: Английский

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Biodegradation of microcystins by microbiota of duckweed Spirodela polyrhiza

Chemosphere, Journal Year: 2024, Volume and Issue: unknown, P. 143436 - 143436

Published: Sept. 1, 2024

Language: Английский

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