A High‐Throughput Ancient DNA Extraction Method for Large‐Scale Sample Screening

Molecular Ecology Resources, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 6, 2025

ABSTRACT Large‐scale DNA screening of palaeontological and archaeological collections remains a limiting costly factor for ancient studies. Several extraction protocols are routinely used in laboratories have even been automated on robotic platforms. Robots offer solution high‐throughput but the costs, as well necessity trained technicians engineers, can be prohibitive some laboratories. Here, we present alternative to robot‐based using 96‐column plate. When compared routine single MinElute columns, retrieved highly similar endogenous contents, an important metric screening. Mitogenomes with coverage depth greater than 0.1× could generated allowed taxonomic assignment. However, average fragment lengths, damage library complexities significantly differed between methods these differences became nonsignificant after modification our purification protocol. Our method allows generation 96 extracts within approximately 4 hours laboratory work while bringing cost down by ~39% columns. Additionally, formally demonstrate that addition Tween‐20 during elution step results higher complexity libraries, thereby enabling genome same sequencing effort.

Language: Английский

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Palaeogenomic inference of biodiversity dynamics across Quaternary timescales

Published: March 18, 2025

Language: Английский

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Adaptation to the High-Arctic island environment despite long-term reduced genetic variation in Svalbard reindeer

iScience, Journal Year: 2023, Volume and Issue: 26(10), P. 107811 - 107811

Published: Sept. 3, 2023

Language: Английский

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4200 Years of Mercury Variation in the Antlers of High-Arctic Wild Reindeer

Published: Jan. 1, 2024

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Language: Английский

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