Persistence of microbiological hazards in food and feed production and processing environments

EFSA Journal, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 1, 2024

Abstract Listeria monocytogenes (in the meat, fish and seafood, dairy fruit vegetable sectors), Salmonella enterica feed, egg low moisture food sectors) Cronobacter sakazakii sector) were identified as bacterial safety hazards most relevant to public health that are associated with persistence in feed processing environment (FFPE). There is a wide range of subtypes these involved FFPE. While some specific more commonly reported persistent, it currently not possible identify universal markers (i.e. genetic determinants) for this trait. Common risk factors FFPE inadequate zoning hygiene barriers; lack hygienic design equipment machines; cleaning disinfection. A well-designed environmental sampling testing programme effective strategy contamination sources detect potentially persistent hazards. The establishment barriers measures within management system, during implementation hazard analysis critical control points, key prevent and/or Once suspected plant, 'seek-and-destroy' approach frequently recommended, including intensified monitoring, introduction continuation monitoring. Successful actions triggered by L. described, well interventions direct bactericidal activity. These could be efficient if properly validated, correctly applied verified under industrial conditions. Perspectives provided performing assessment combinations sector assess relative can persistence, based on bottom-up top-down approaches. Knowledge gaps related priorities future research provided.

Language: Английский

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The Origin of Niches and Species in the Bacterial World

Frontiers in Microbiology, Journal Year: 2021, Volume and Issue: 12

Published: March 17, 2021

Niches are spaces for the biological units of selection, from cells to complex communities. In a broad sense, “species” individuation. do not exist without individual organisms, and every organism has niche. We use “niche” in Hutchinsonian sense as an abstraction multidimensional environmental space characterized by variety conditions, both biotic abiotic, whose quantitative ranges determine positive or negative growth rates microbial individual, typically species, but also parts communities species contained this space. Microbial organisms (“species”) constantly diversify, such diversification (radiation) depends on possibility opening up unexploited insufficiently exploited niches. Niche exploitation frequently implies “niche construction,” colonized niche evolves with time, giving rise new potential subniches, thereby influencing selection series variants progeny. The evolution niches is result reciprocal interacting processes that form single unified process. Centrifugal expands limits species’ while centripetal cohesive process occurs simultaneously, mediated horizontal gene transfers recombinatorial events, condensing all information recovered during diversifying specialization into “novel organisms” (possible future species), creating more niche, where selfishness organism(s) establishes “homeostatic power” limiting niche’s variation. Once full carrying capacity been reached, reproductive isolation occurs, no foreign can outcompete established population/community, facilitating speciation. case individualization-speciation microbiota, its contribution animal’ gut structure type crosstalk between (host) microorganism(s). Lastly, there parallelism hierarchy individuals. increasing anthropogenic effects biosphere (such globalization) might reduce diversity bacterial individuals, emergence highly transmissible multispecialists (which eventually deleterious) resulting homogenization microbiosphere, should be explored prevented.

Language: Английский

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Evolutionary Pathways and Trajectories in Antibiotic Resistance

Clinical Microbiology Reviews, Journal Year: 2021, Volume and Issue: 34(4)

Published: June 30, 2021

Evolution is the hallmark of life. Descriptions evolution microorganisms have provided a wealth information, but knowledge regarding "what happened" has precluded deeper understanding "how" proceeded, as in case antimicrobial resistance. The difficulty answering question lies multihierarchical dimensions evolutionary processes, nested complex networks, encompassing all units selection, from genes to communities and ecosystems. At simplest ontological level (as resistance genes), proceeds by random (mutation drift) directional (natural selection) processes; however, sequential pathways adaptive variation can occasionally be observed, under fixed circumstances (particular fitness landscapes), predictable. highest (such that plasmids, clones, species, microbiotas), systems' degrees freedom increase dramatically, related variable dispersal, fragmentation, relatedness, or coalescence bacterial populations, depending on heterogeneous changing niches selective gradients environments. Evolutionary trajectories antibiotic find their way these landscapes subjected variations, becoming highly entropic therefore unpredictable. However, experimental, phylogenetic, ecogenetic analyses reveal preferential frequented paths (highways) where flows propagates, allowing some dynamics, modeling designing interventions. Studies an applied aspect improving individual health, One Health, Global well academic value for evolution. Most importantly, they heuristic significance model reduce negative influence anthropogenic effects environment.

Language: Английский

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Link Between Antibiotic Persistence and Antibiotic Resistance in Bacterial Pathogens

Frontiers in Cellular and Infection Microbiology, Journal Year: 2022, Volume and Issue: 12

Published: July 19, 2022

Both, antibiotic persistence and resistance characterize phenotypes of survival in which a bacterial cell becomes insensitive to one (or even) more antibiotic(s). However, the molecular basis for these two antibiotic-tolerant is fundamentally different. Whereas genetically determined hence represents rather stable phenotype, marks transient physiological state triggered by various stress-inducing conditions that switches back original sensitive once environmental situation improves. The basics are principle well understood. This not case persistence. Under all culture conditions, there stochastically formed, subpopulation persister cells populations, size depends on conditions. proportion persisters population increases under different stress including treatment with bactericidal antibiotics (BCAs). Various models have been proposed explain formation bacteria. We recently hypothesized leading converge inability bacteria re-initiate new round DNA replication caused an insufficient level initiator complex ATP-DnaA lack functional orisome. Here, we extend this hypothesis proposing become susceptible mutation-based provided they equipped error-prone repair functions. - our opinion particular when such populations exposed BCAs.

Language: Английский

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Recent Advances in Electrochemical Biosensors for the Detection of Foodborne Pathogens: Current Perspective and Challenges

Foods, Journal Year: 2023, Volume and Issue: 12(14), P. 2795 - 2795

Published: July 23, 2023

Foodborne pathogens cause many diseases and significantly impact human health the economy. mainly include Salmonella spp., Escherichia coli, Staphylococcus aureus, Shigella Campylobacter spp. Listeria monocytogenes, which are present in agricultural products, dairy animal-derived foods environment. Various different types of food water can potentially life-threatening develop resistance to various antibiotics. The harm foodborne is increasing, necessitating effective efficient methods for early monitoring detection. Traditional methods, such as real-time polymerase chain reaction (RT-PCR), enzyme-linked immunosorbent assay (ELISA) culture plate, time-consuming, labour-intensive expensive cannot satisfy demands rapid testing. Therefore, new fast detection urgently needed. Electrochemical biosensors provide consumer-friendly quickly detect environment achieve extensive accuracy reproducible results. In this paper, by focusing on mechanisms electrochemical transducers, we a comprehensive overview pathogens. Furthermore, review introduces hazards pathogens, risk analysis measures control. Finally, also emphasizes recent research progress solutions regarding use environment, evaluates limitations challenges experienced during development discusses future possibilities.

Language: Английский

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trans-Cinnamaldehyde as a Novel Candidate to Overcome Bacterial Resistance: An Overview of In Vitro Studies

Antibiotics, Journal Year: 2023, Volume and Issue: 12(2), P. 254 - 254

Published: Jan. 27, 2023

The increasing of drug-resistant bacteria and the scanty availability novel effective antibacterial agents represent alarming problems modern society, which stimulated researchers to investigate strategies replace or assist synthetic antibiotics. A great deal attention has been devoted over years essential oils that contain mixtures volatile compounds have traditionally exploited as antimicrobial remedies. Among oil phytochemicals, remarkable antibiotic-potentiating activities highlighted for cinnamaldehyde, an α,β-unsaturated aldehyde, particularly abundant in Cinnamomum spp., widely used a food additive industrial products. In line with this evidence, present study, overview available literature carried out order define bacterial sensitizing profile cinnamaldehyde. vitro studies displayed ability substance resensitize microbial strains drugs increase efficacy different antibiotics, especially cefotaxime, ciprofloxacin, gentamicin; however, vivo, clinical trials are lacking. Based on collected findings, cinnamaldehyde appears be interest adjuvant agent overcome superbug infections antibiotic resistance; future more in-dept investigations should encouraged clarify its mechanisms involved.

Language: Английский

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Phenotypic and genotypic antimicrobial resistance of Listeria monocytogenes: an observational study in France

The Lancet Regional Health - Europe, Journal Year: 2023, Volume and Issue: 37, P. 100800 - 100800

Published: Dec. 14, 2023

Large-scale studies are needed to clarify antimicrobial resistance in the foodborne pathogen

Language: Английский

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Antimicrobial Resistance of Listeria monocytogenes Strains Isolated in Food and Food-Processing Environments in Italy

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 525 - 525

Published: June 3, 2024

Listeria monocytogenes, along with various other pathogenic bacteria, may show resistance against a broad spectrum of antibiotics. Evaluating the extent in harmful microorganisms like monocytogenes holds significant importance crafting novel therapeutic strategies to mitigate or combat rise infections stemming from antibiotic-resistant bacteria. The present work aims investigate occurrence antimicrobial among strains meat products (n = 173), seafood 54), dairy 19), sauces 2), confectionary 1), ready-to-eat rice dishes and food-processing environments 19). A total 269 belonging eight different serovars were tested 10 antimicrobials. In classes antibiotics, most resistant antibiotics family β-lactams (92.94%). High proportions L. isolates oxacillin (88.48%), followed by fosfomycin (85.87%) flumenique (78.44%). lowest level was observed gentamycin (1.49%). 235 87.36%) showed profile multidrug resistance. conclusion, high multidrug-resistant examined serotypes isolated food sources. This understanding enables adoption suitable measures avert contamination spread bacteria via food.

Language: Английский

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The prospect of using nanotechnology to prevent and treat infections caused by Listeria monocytogenes

Critical Reviews in Microbiology, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 9

Published: Jan. 15, 2025

Listeria monocytogenes (L. monocytogenes) is an opportunistic intracellular pathogen that causes listeriosis in human and leads to high mortality rate. L. resistant various antibiotics due its ability form biofilm. Designing a new generation of very expensive time-consuming process. Moreover, the protection via drug delivery system can promote their effectiveness bioavailability. Nanomedicine be promising tool for treating bacteria preventing recurrence infections. Nanocarriers employed as antibacterial agents or carrier agents. In present review, application nanotechnology has been discussed prevention treatment infection. According studies, nanomaterials potential strategy eradicate infections caused by monocytogenes.

Language: Английский

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Unraveling the emergence and population diversity of Listeria monocytogenes in a newly built meat facility through whole genome sequencing

International Journal of Food Microbiology, Journal Year: 2021, Volume and Issue: 340, P. 109043 - 109043

Published: Jan. 8, 2021

Language: Английский

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