Competition between Pseudomonas aeruginosa and Staphylococcus aureus is dependent on intercellular signaling and regulated by the NtrBC two-component system DOI Creative Commons
Morgan A. Alford, Simranpreet Kaur Mann,

Noushin Akhoundsadegh

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 30, 2022

Abstract Pseudomonas aeruginosa and Staphylococcus aureus are often comorbid human pathogens, isolated from expectorated sputum of cystic fibrosis patients chronically infected wounds. Prior studies revealed a competitive advantage P. over S. in vitro that was slightly muted vivo. Here, we demonstrated the two-component regulatory system NtrBC influences skin organoid mouse models co-infection. Expression ntrBC induced during co-culture two species could be recapitulated monoculture by addition metabolite N-acetylglucosamine is released following lysis. LESB58 WT, but not mutant (Δ ntrC Δ ) strains, lysis USA300 LAC planktonic growth outcompeted biofilm formation vitro. We confirmed these findings murine abscess model high-density infection. Accordingly, secretory profile mutants reduced production anti-staphylococcal virulence factors including pyoverdine, pyocyanin elastase. These phenotypes at least partly complemented overexpression quorum sensing molecules homoserine lactones or alkylquinolone signaling molecules. data implicate complex cascade triggered interspecies gives edge LAC.

Language: Английский

Complex Chronic Wound Biofilms Are Inhibited in vitro by the Natural Extract of Capparis spinose DOI Creative Commons
Silvia Di Lodovico, Tiziana Bacchetti, Simonetta D’Ercole

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: April 11, 2022

Resistant wound microorganisms are becoming an extremely serious challenge in the process of treating infected chronic wounds, leading to impaired healing. Thus, additional approaches should be taken into consideration improve healing process. The use natural extracts can represent a valid alternative treat/control microbial infections wounds. This study investigates antimicrobial/antivirulence effects Capparis spinose aqueous extract against main pathogens: Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. shows phenolic characterization with rutin (1.8 ± 0.14 μg/mg) as major compound antibacterial effect bacteria (S. aureus PECHA 10 MIC 6.25%; P. aeruginosa 4 12.50%) without action C. albicans (MIC MFC ≥ 50%). also significant antivirulence terms antimotility/antibiofilm actions. In particular, acts (i) on both increasing its swimming swarming motility favoring planktonic phenotype reducing adhesive capability, (ii) S. biofilm formation biomass CFU/ml. Furthermore, significantly displays reduction dual-species Lubbock biofilm, complex model that mimics realistic vivo spatial distribution results suggest could innovative eco-friendly strategy prevent/control infection.

Language: Английский

Citations

14
Genetic and environmental determinants of surface adaptations in Pseudomonas aeruginosa DOI
Sakthivel Ambreetha, Varsha Singh

Microbiology, Journal Year: 2023, Volume and Issue: 169(6)

Published: June 5, 2023

Pseudomonas aeruginosa is a well-studied Gram-negative opportunistic bacterium that thrives in markedly varied environments. It nutritionally versatile microbe can colonize host as well exist the environment. Unicellular, planktonic cells of P. come together to perform coordinated swarming movement or turn into sessile, surface-adhered population called biofilm. These collective behaviours produce strikingly different outcomes. While motility rapidly disseminates bacterial population, biofilm collectively protects from environmental stresses such heat, drought, toxic chemicals, grazing by predators, and attack immune antibiotics. The ubiquitous nature likely be supported timely transition between planktonic, lifestyles. social this bacteria viz swarm modes are controlled signals quorum-sensing networks, LasI-LasR, RhlI-RhlR PQS-MvfR, several other sensory kinases response regulators. A combination genetic cues regulates specific states. current review aimed at discussing key factors promote physiologically distinct transitioning population.

Language: Английский

Citations

7
Intense pulsed light for inactivation of foodborne gram-positive bacteria in planktonic cultures and bacterial biofilms DOI
Ziqi Liu, Shuaifeng Hu, Thanapop Soteyome

et al.

LWT, Journal Year: 2021, Volume and Issue: 152, P. 112374 - 112374

Published: Aug. 30, 2021

Language: Английский

Citations

16
The interplay of Pseudomonas aeruginosa and Staphylococcus aureus in dual-species biofilms impacts development, antibiotic resistance and virulence of biofilms in in vitro wound infection models DOI Creative Commons
Pia Katharina Vestweber, Jana Wächter, Viktoria Planz

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0304491 - e0304491

Published: May 28, 2024

Due to high tolerance antibiotics and pronounced virulence, bacterial biofilms are considered a key factor major clinical challenge in persistent wound infections. They typically composed of multiple species, whose interactions determine the biofilm’s structural development, functional properties thus progression However, most attempts study vitro solely rely on mono-species populations, since cultivating multi-species biofilms, especially for prolonged periods time, poses significant challenges. To address this, present examined influence composition biofilm morphology spatial organization, as well antibiotic virulence human skin cells context By creating wound-mimetic microenvironment, successful cultivation dual-species two prevalent pathogens, Pseudomonas aeruginosa Staphylococcus aureus , was realized over period 72 h. Combining quantitative analysis with electron microscopy label-free imaging enabled comprehensive evaluation dynamics formation matrix secretion, revealing twofold increased maturation biofilms. Antibiotic comparable both cultures, however, communities showed 50% increase tolerance, mediated by significantly reduced penetration applied into matrix. Further synergistic effects were observed, where exacerbated healing beyond observed from either or . Consequently, predicting antimicrobial based results is unreliable. This underscores substantial impact emphasizes need tailor future studies reflecting respective vivo situation, leading more understanding microbial basic microbiology development effective treatments.

Language: Английский

Citations

2
Competition between Pseudomonas aeruginosa and Staphylococcus aureus is dependent on intercellular signaling and regulated by the NtrBC two-component system DOI Creative Commons
Morgan A. Alford, Simranpreet Kaur Mann,

Noushin Akhoundsadegh

et al.

Scientific Reports, Journal Year: 2022, Volume and Issue: 12(1)

Published: May 30, 2022

Abstract Pseudomonas aeruginosa and Staphylococcus aureus are often comorbid human pathogens, isolated from expectorated sputum of cystic fibrosis patients chronically infected wounds. Prior studies revealed a competitive advantage P. over S. in vitro that was slightly muted vivo. Here, we demonstrated the two-component regulatory system NtrBC influences skin organoid mouse models co-infection. Expression ntrBC induced during co-culture two species could be recapitulated monoculture by addition metabolite N-acetylglucosamine is released following lysis. LESB58 WT, but not mutant (Δ ntrC Δ ) strains, lysis USA300 LAC planktonic growth outcompeted biofilm formation vitro. We confirmed these findings murine abscess model high-density infection. Accordingly, secretory profile mutants reduced production anti-staphylococcal virulence factors including pyoverdine, pyocyanin elastase. These phenotypes at least partly complemented overexpression quorum sensing molecules homoserine lactones or alkylquinolone signaling molecules. data implicate complex cascade triggered interspecies gives edge LAC.

Language: Английский

Citations

10