Exploring the Biocontrol Capability of Non-Mycotoxigenic Strains of Penicillium expansum

Toxins, Journal Year: 2024, Volume and Issue: 16(1), P. 52 - 52

Published: Jan. 17, 2024

Penicillium expansum is one the major postharvest pathogens of pome fruit during handling and storage. This fungus also produces patulin, which a highly toxic mycotoxin that can contaminate infected fruits their derived products whose levels are regulated in many countries. In this study, we investigated biocontrol potential non-mycotoxigenic strains against mycotoxigenic strain. We analyzed competitive behavior two knockout mutants were unable to produce patulin. The first mutant (∆patK) involved deletion patK gene, initial gene patulin biosynthesis. second (∆veA) veA, global regulator primary secondary metabolism. At phenotypic level, ∆patK exhibited similar characteristics wild-type contrast, ∆veA displayed altered growth compared with wild type, including reduced conidiation abnormal conidiophores. Neither produced under tested conditions. Under various stress conditions, when exposed stressors, cell membrane stress, oxidative osmotic different pH values. However, no significant changes observed mutant. experiments, presence population strain vitro growth. Furthermore, addition either resulted decrease levels. Overall, our results suggest use mutants, particularly as agents reduce contamination food feed.

Language: Английский

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Comparative physiological and transcriptomic analysis reveals an improved biological control efficacy of Sporidiobolus pararoseus Y16 enhanced with ascorbic acid against the oxidative stress tolerance caused by Penicillium expansum in pears

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 210, P. 108627 - 108627

Published: April 19, 2024

Language: Английский

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Antifungal properties and molecular docking of ZnO NPs mediated using medicinal plant extracts

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 5, 2024

Significant postharvest losses and food safety issues persist in many developing nations, primarily due to fungal activities, including mycotoxin production. In this study, green synthesised zinc oxide nanoparticles (ZnO-NPs) were prepared from leaf extracts of Syzygium cordatum (ZnO 1), Lippia javanica 2), Bidens pilosa 3), Ximenia caffra 4). Physicochemical characteristics the ZnO-NPs determined using X-ray diffraction (XRD), Fourier transmission Infrared spectroscopy ultraviolet–visible (UV–vis) spectroscopy, scanning electron microscopy (SEM), (TEM). The XRD analysis confirmed presence a wurtzite crystal structure hexagonal shape ZnO (NPs), with an average size ranging between 25 43 nm. microscopic examination morphology revealed spherical particles sizes 37 47 nm diameter. antifungal efficacy was assessed against pathogenic plant fungi, Botrytis sp. (STEU 7866), Penicillium 7865), Pilidiella granati 7864), poisoned technique. Further evaluation ZnOPs performed broth microdilution assay. A significant interaction type species observed, highest susceptibility Mucor 2, achieving over 50% inhibition. also showed high all ZnO-NPs. Molecular docking results strong H-bonding interactions receptors sp., P. exhibited least susceptibility. tests that 2 inhibitory effect on low minimum concentration (MIC) µg/mL, attributed its larger positive zeta potential. This study indicates NPs, particularly those mediated have promising potential as effective agents, which could play role reducing decay losses.

Language: Английский

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Cinnamon Oil Inhibits Penicillium expansum Growth by Disturbing the Carbohydrate Metabolic Process

Journal of Fungi, Journal Year: 2021, Volume and Issue: 7(2), P. 123 - 123

Published: Feb. 9, 2021

Penicillium expansum is a major postharvest pathogen that mainly threatens the global pome fruit industry and causes great economic losses annually. In present study, antifungal effects potential mechanism of cinnamon oil against P. were investigated. Results indicated 0.25 mg L−1 could efficiently inhibit spore germination, conidial production, mycelial accumulation, expansion expansum. addition, it effectively control blue mold rots induced by in apples. Cinnamon also reduce expression genes involved patulin biosynthesis. Through proteomic quantitative analysis, total 146 differentially expressed proteins (DEPs) carbohydrate metabolic process, most which down-regulated, noticed for their large number functional significance. Meanwhile, expressions 14 candidate corresponding to DEPs activities six key regulatory enzymes (involving cellulose hydrolyzation, Krebs circle, glycolysis, pentose phosphate pathway) showed similar trend protein levels. extracellular consumption, intracellular ATP production under stress significantly decreased. Basing on correlated mutually authenticated results, we speculated disturbing fungal process would be partly responsible inhibitory growth. The findings provide new insights into antimicrobial mode oil.

Language: Английский

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Ultrastructural observation and transcriptome analysis provide insights into mechanisms of Penicillium expansum invading apple wounds

Food Chemistry, Journal Year: 2023, Volume and Issue: 414, P. 135633 - 135633

Published: Feb. 6, 2023

Language: Английский

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