Camellia oleifera CoSWEET10 Is Crucial for Seed Development and Drought Resistance by Mediating Sugar Transport in Transgenic Arabidopsis

Plants, Journal Year: 2023, Volume and Issue: 12(15), P. 2818 - 2818

Published: July 29, 2023

Sugar transport from the source leaf to sink organ is critical for seed development and crop yield, as well responding abiotic stress. SWEETs (sugar will eventually be exported transporters) mediate sugar efflux into reproductive are therefore considered key candidate proteins unloading during development. However, specific mechanism underlying seeds in Camellia oleifera remains elusive. Here, we identified a SWEET gene named CoSWEET10, which belongs Clade III has high expression levels of C. oleifera. CoSWEET10 plasma membrane-localized protein. The complementation assay SUSY7/ura3 EBY.VW4000 yeast strains showed that ability sucrose, glucose, fructose. Through vitro culture, found can induced by hexose especially glucose. By generating restoration lines Arabidopsis atsweet10, restored defect phenotype mutant regulating soluble accumulation increased plant drought tolerance. Collectively, our study demonstrates plays dual role promoting enhancing resistance sucrose transporter.

Language: Английский

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Carbon fluxes and environmental interactions during legume development, with a specific focus on Pisum sativum

Physiologia Plantarum, Journal Year: 2022, Volume and Issue: 174(3)

Published: May 1, 2022

Abstract Grain legumes are major food crops cultivated worldwide for their seeds with high nutritional content. To answer the growing concern about safety and protein autonomy, legume cultivation must increase in coming years. In parallel, current agricultural practices facing environmental challenges, including global temperature more frequent severe episodes of drought stress. Crop yield directly relies on carbon allocation is particularly affected by these changes. We review knowledge source‐sink relationships resource at all developmental stages, from germination to vegetative growth seed production grain legumes, focusing pea ( Pisum sativum ). also discuss how fluxes influenced biotic abiotic factors. Major agronomic traits, quality, impacted drought, temperatures, salinity, waterlogging, or pathogens can be improved through promotion beneficial soil microorganisms optimized plant allocation. Altogether, our highlights need a better understanding cellular molecular mechanisms regulating source leaves sink organs, roots, seeds. These advancements will further improve stability stress tolerance contribute selection climate‐resilient crops.

Language: Английский

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Multi-Omics Approaches to Improve Clubroot Resistance in Brassica with a Special Focus on Brassica oleracea L.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(16), P. 9280 - 9280

Published: Aug. 17, 2022

is an agronomically important species of the

Language: Английский

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Precision Genome Editing Toolbox: Applications and Approaches for Improving Rice’s Genetic Resistance to Pathogens

Agronomy, Journal Year: 2022, Volume and Issue: 12(3), P. 565 - 565

Published: Feb. 24, 2022

In the present scenario of a looming food crisis, improving per hectare rice productivity at greater pace is among topmost priorities scientists and breeders. past decades, conventional, mutational, marker-assisted breeding techniques have played significant role in developing multiple desired varieties. However, due to certain limitations, these cannot furnish projected security 2050 population’s aching stomachs. One possible options would be precise crop genome editing using various tools, viz., TALENs CRISPR/Cas9 resolve this multifaceted crisis. Initially, potentiality technologies was tested only protoplasts. Later, were employed edit calli with help modified vectors, CRISPR variants, cassette cloning systems, delivery methods. With continuous technological advancements such as base editing, multiplexing, etc., precision, rapidness, efficiency, reliability, potency, range applications platforms increased even been used for gene function studies. This leads revolution field improvement program, especially stress tolerance against pests pathogens which susceptibility factors located within are targeted through tools. Therefore, current article, we summarized tools during last decade concerning enhanced biotic tolerance. Additionally, focused on regulatory aspects associated risks prospects reshape durable resistance complex stress.

Language: Английский

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Plasmodiophora brassicae affects host gene expression by secreting the transcription factor-type effector PbZFE1

Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(1), P. 454 - 467

Published: Sept. 21, 2023

Abstract The protist pathogen Plasmodiophora brassicae hijacks the metabolism and development of host cruciferous plants induces clubroot formation, but little is known about its regulatory mechanisms. Previously, Pnit2int2 sequence, a sequence around second intron nitrilase gene (BrNIT2) involved in auxin biosynthesis Brassica rapa ssp. pekinensis, was identified as specific promoter activated during formation. In this study, we hypothesized that analysis transcriptional regulation could reveal how P. affects system development. By yeast one-hybrid screening, zinc finger protein PbZFE1 to specifically bind Pnit2int2. Specific binding also confirmed by electrophoretic mobility shift assay. site essential for activity clubbed roots transgenic Arabidopsis thaliana (Pnit2int2-2::GUS), indicating secreted from functions within plant cells. Ectopic expression PbZEF1 A. delayed growth flowering time, suggesting has significant impacts on metabolic systems. Thus, appears secrete into cells transcription factor-type effector pathogenesis.

Language: Английский

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