The regulation of auxin receptor gene CsAFB2 by csn‐miR393a confers resistance against Colletotrichum gloeosporioides in tea plants DOI Creative Commons
Anburaj Jeyaraj, Shujing Liu, Rui Han

et al.

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(4)

Published: March 28, 2025

Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression response resistance, defence responses plant immunity. However, the regulatory mechanisms of miRNAs Colletotrichum gloeosporioides remain unknown tea plants. Our study revealed that csn-miR393a targets auxin receptor CsAFB2 during resistance C. plants comparing resistant cultivar Zhongcha108 susceptible Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated suppresses CsAFB2, target mimic blocks function csn-miR393a, leading increase CsAFB2. Repression transcripts leaves antisense oligonucleotides negatively regulating reactive oxygen species homoeostasis, PR catechin accumulation. To further validate developed transgenic overexpressing resulting enhanced against gloeosporioides. Additionally, N. lines provided evidence regulates suppressing Therefore, manipulating or its gene, has potential strengthen plant's anthracnose.

Language: Английский

The regulation of auxin receptor gene CsAFB2 by csn‐miR393a confers resistance against Colletotrichum gloeosporioides in tea plants DOI Creative Commons
Anburaj Jeyaraj, Shujing Liu, Rui Han

et al.

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(4)

Published: March 28, 2025

Anthracnose, a severe disease caused by Colletotrichum, affects diverse crops and leads to significant economic losses through pronounced fruit/leaf lesions. MicroRNAs (miRNAs) play crucial roles in modulating gene expression response resistance, defence responses plant immunity. However, the regulatory mechanisms of miRNAs Colletotrichum gloeosporioides remain unknown tea plants. Our study revealed that csn-miR393a targets auxin receptor CsAFB2 during resistance C. plants comparing resistant cultivar Zhongcha108 susceptible Longjing43. Through Nicotiana benthamiana leaf co-transformation assays, we demonstrated suppresses CsAFB2, target mimic blocks function csn-miR393a, leading increase CsAFB2. Repression transcripts leaves antisense oligonucleotides negatively regulating reactive oxygen species homoeostasis, PR catechin accumulation. To further validate developed transgenic overexpressing resulting enhanced against gloeosporioides. Additionally, N. lines provided evidence regulates suppressing Therefore, manipulating or its gene, has potential strengthen plant's anthracnose.

Language: Английский

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