Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(1), P. 77 - 77

Published: Jan. 18, 2025

This review delves into innovative technologies to improve the control of vascular fungal plant pathogens. It also briefly summarizes traditional biocontrol approaches manage them, addressing their limitations and emphasizing need develop more sustainable precise solutions. Powerful tools such as next-generation sequencing, meta-omics, microbiome engineering allow for targeted manipulation microbial communities enhance pathogen suppression. Microbiome-based include design synthetic consortia transplant entire or customized soil/plant microbiomes, potentially offering resilient adaptable strategies. Nanotechnology has advanced significantly, providing methods delivery biological agents (BCAs) compounds derived from them through different nanoparticles (NPs), including bacteriogenic, mycogenic, phytogenic, phycogenic, debris-derived ones acting carriers. The use biodegradable polymeric non-polymeric eco-friendly NPs, which enable controlled release antifungal while minimizing environmental impact, is explored. Furthermore, artificial intelligence machine learning can revolutionize crop protection early disease detection, prediction outbreaks, precision in BCA treatments. Other genome editing, RNA interference (RNAi), functional peptides efficacy against pathogenic fungi. Altogether, these provide a comprehensive framework management diseases, redefining modern agriculture.

Language: Английский

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Harnessing RNA interference for the control of Fusarium species: A critical review

Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(10)

Published: Oct. 1, 2024

Fusarium fungi are a pervasive threat to global agricultural productivity. They cause spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins harmful human animal health. In recent years, the exploitation RNA interference (RNAi) mechanism has emerged as promising avenue for control Fusarium-induced diseases, providing both mechanistic understanding gene function potential strategy environmentally sustainable disease management. However, despite progress elucidating presence RNAi pathway different species, comprehensive its individual protein components underlying silencing mechanisms remains elusive. Accordingly, while considerable number RNAi-based approaches have been developed many reports applications under laboratory conditions published, applicability this knowledge agronomic settings an open question, few convincing data on field published. This review aims consolidate current role evaluating research highlighting important avenues future investigation.

Language: Английский

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Designer antisense circRNAGFP reduces GFP abundance in Arabidopsis protoplasts in a sequence-specific manner, independent of RNAi pathways

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Circular RNAs (circRNAs) are single-stranded RNA molecules characterised by their covalently closed structure and emerging as key regulators of cellular processes in mammals, including gene expression, protein function immune responses. Recent evidence suggests that circRNAs also play significant roles plants, influencing development, nutrition, biotic stress resistance, abiotic tolerance. However, the potential to modulate target abundance plants remains largely unexplored. In this study, we investigated designer using Arabidopsis a model system. We demonstrate treatment with 50 nt circRNA_GFP, containing 30 GFP antisense sequence stretch, results reduced reporter dose- sequence-dependent manner. Notably, open isoform circRNA_GFP had little effect on abundance, indicating importance circular structure. Additionally, mutants defective interference (RNAi), suggesting circRNA activity is independent RNAi pathway. show circRNA, unlike dsRNA, does not induce pattern-triggered immunity (PTI) plants. Findings proof-of-principle study together crucial first steps understanding versatile tools for modulating expression offer exciting prospects application agronomy, particularly enhancing crop traits through metabolic pathway manipulation.

Language: Английский

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Control of Fusarium graminearum Infection in Wheat by dsRNA-Based Spray-Induced Gene Silencing

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Spray-induced gene silencing (SIGS) has become a new technology for pest and disease control in plants. This study synthesized three double-strand RNAs (dsRNAs) targeting Fusarium graminearum (F. graminearum), the major pathogen causing head blight (FHB). Co-incubation showed weak uptake of dsRNA by F. graminearum, some dsRNAs influence spore germination hyphae growth. In contrast, exogenous quickly efficiently penetrates wheat leaves. Treatment leaves detached heads with these negative effect on pathogenicity graminearum. Foliar spraying dsCHS3b or dsMGV1 decreased amount artificially inoculated incidence rate, severity field. Under natural conditions, significantly FHB index deoxynivalenol content. Twice spray achieved more than 90% FHB. conclusion, SIGS effectively prevents infection wheat, providing green way control.

Language: Английский

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