Identification of immune-related biomarkers and immune infiltrations of intracranial aneurysm with subarachnoid hemorrhage by machine-learning strategies

Computer Methods in Biomechanics & Biomedical Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: April 23, 2025

Background: Subarachnoid hemorrhage (SAH) risk increases with intracranial aneurysms (IA), but their relationship remains unclear. Methods: We explored SAH-IA links using machine learning and bioinformatics, identifying 66 IA-related SAH genes. KEGG analysis highlighted pathways like NF-κB, TNF, COVID-19. Results: Two immune-related genes (ZNF281, LRRN3) were identified, a ceRNA network was constructed. Ten potential drugs screened via CMAP. Conclusion: ZNF281 LRRN3 may regulate immune (T cells, NK macrophages), influencing development, could serve as therapeutic targets.

Language: Английский

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Macrophage ferroptosis inhibits Aspergillus conidial killing in lung transplantation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 15, 2025

Abstract Immune suppression heightens the risk for fungal infections, but mechanisms that result in clinical disease are poorly understood. Here we demonstrate macrophage ferroptosis, an iron-dependent form of regulated cell death, inhibits Aspergillus fumigatus ( Af ) killing. In a mouse tracheal transplant model infection, observed increase lipid peroxidation, decreased expression negative ferroptosis regulators Gpx4 and Slc7a11 , positive Ptgs2 Nox2 relative to syntransplants. Depletion macrophages recipients invasion. vitro iron overload reduced viability their capability kill spores, through decrease lysosomal acidification loss. Treatment with ferrostatin-1, inhibitor, deferasirox (an chelator) restored Ferroptotic alveolar isolated from lung patients also showed ability spores patients’ bronchoalveolar lavage was characterized by higher levels markers ferroptotic stress compared non-lung transplants. These characteristics were strongly correlated history independent immune suppressive medications. Our findings indicate augments invasive aspergillosis, representing novel mechanism host dysfunction. Graphical Schematic proposed underlying induced dysregulation increased invasion transplantation.

Language: Английский

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Non-Aspergillus Pulmonary Mold Infections

Published: Jan. 1, 2024

Language: Английский

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