Validation of suitable reference microRNAs for qRT-PCR in Osmanthus fragrans under abiotic stress, hormone and metal ion treatments

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 14, 2025

Introduction Sweet osmanthus ( Osmanthus fragrans ) is a prominent woody ornamental plant extensively utilized in horticulture, the food industry, cosmetics, and traditional Chinese medicine. MicroRNAs (miRNAs) are crucial regulators of gene regulation, playing vital role enabling plants to adapt environmental fluctuations. Despite their significance, research on miRNA expression O. under adverse stress conditions remains limited. Therefore, selection appropriate reference miRNAs essential ensure accurate analysis. Methods In this study, qRT-PCR technology was combined with four algorithms (i.e., delta-Ct, geNorm, NormFinder, BestKeeper) systematically evaluate stability 14 candidate across eleven conditions, including abiotic stress, hormone metal ion treatments, during flower opening senescence, various tissues. Results The results revealed that ofr-miR159b-3p, novel8, novel3 exhibited high stability; ofr-miR403-3p, novel2 demonstrated considerable novel3, novel33, novel2, ofr-miR395e were identified as stable miRNAs; different tissues, relatively stable; novel33 maintained expression. Discussion This study represents first comprehensive assessment , providing reliable framework for analysis diverse development treatments. These findings carry significant implications future into function miRNAs.

Language: Английский

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MicroRNA gatekeepers: Orchestrating rhizospheric dynamics

Journal of Integrative Plant Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

ABSTRACT The rhizosphere plays a crucial role in plant growth and resilience to biotic abiotic stresses, highlighting the complex communication between plants their dynamic environment. Plants produce wide range of signaling molecules that facilitate with various factors, yet our understanding these mechanisms remains elusive. In addition protein‐coding genes, increasing evidence underscores critical microRNAs (miRNAs), class non‐coding single‐stranded RNA molecules, regulating growth, development, responses stresses under diverse factors. this review, we explore crosstalk miRNAs target mRNAs, which influence development key structures shaped by belowground Moving forward, more focused studies are needed clarify functions expression patterns miRNAs, uncover common regulatory mediate tolerance dynamics. Beyond that, propose using artificial manipulating targets through overexpression or knockout/knockdown approaches could effectively investigate roles offering significant potential for advancing crop engineering.

Language: Английский

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MicroRNAs: emerging regulators in horticultural crops

Trends in Plant Science, Journal Year: 2022, Volume and Issue: 27(9), P. 936 - 951

Published: April 21, 2022

Language: Английский

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The master role of siRNAs in plant immunity

Molecular Plant Pathology, Journal Year: 2022, Volume and Issue: 23(10), P. 1565 - 1574

Published: July 22, 2022

Abstract Gene silencing mediated by small noncoding RNAs (sRNAs) is a fundamental gene regulation mechanism in eukaryotes that broadly governs cellular processes. It has been established sRNAs are critical regulators of plant growth, development, and antiviral defence, while accumulating studies support positive roles defence against bacteria eukaryotic pathogens such as fungi oomycetes. Emerging evidence suggests move between species function antimicrobial agents nonviral parasites. Multiple pathosystems have shown to involve similar exchange species. Recent analysis about extracellular shed light on the understanding selection transportation moving from In this review, we summarize current advances regarding regulatory endogenous interfering (siRNAs) mediating pathogen intruders including viruses, bacteria, fungi, oomycetes, parasitic plants. Beyond that, propose potential mechanisms behind sorting idea engineering siRNA‐producing loci could be useful strategy improve disease resistance crops.

Language: Английский

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Review: Plant microRNAs in pathogen defense: A panacea or a piece of the puzzle?

Plant Science, Journal Year: 2024, Volume and Issue: 341, P. 111993 - 111993

Published: Jan. 22, 2024

Plant microRNAs (miRNAs) control key agronomic traits that are associated with their conserved role(s) in development. However, despite a multitude of studies, the utility miRNAs plant-pathogen resistance remains less certain. Reviewing literature identifies three general classes regarding plant pathogen defense. Firstly, number evolutionary dynamic 22 nucleotide miRNA families repress large numbers immunity genes, either directly, or through triggering biogenesis secondary siRNAs. understanding role defense and manipulation to enhance still lacking. Secondly, highly indirectly impact disease targets primarily regulating development hormone signaling. Any alteration these usually results pleiotropic impacts, which may alter some species, against pathogens. Thirdly, comparatively diverse set non-conserved miRNAs, contribute resistance, but whose narrow presence will likely restrict utility. Therefore, reflecting evolving nature interactions, complex interplay responses exists. miRNA-based solution for be specific, rather than panacea.

Language: Английский

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A novel long noncoding RNA CIL1 enhances cold stress tolerance in Arabidopsis

Plant Science, Journal Year: 2022, Volume and Issue: 323, P. 111370 - 111370

Published: July 2, 2022

Language: Английский

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CRISPR/Cas9‐mediated saturated mutagenesis of the cotton MIR482 family for dissecting the functionality of individual members in disease response

Plant Direct, Journal Year: 2022, Volume and Issue: 6(6)

Published: June 1, 2022

Many genes encoding nucleotide-binding leucine-rich repeat receptors (NLRs) are regulated and fine-tuned by miR482 to balance the trade-off between disease resistance growth. Dicotyledonous plants, including cotton, usually have multiple isoforms. Each isoform can regulate several NLRs that in turn be different Dissecting functionality of individual isoforms response balancing growth demands a collection mutants mutated members (single or multiple). In this study, we generated such cotton using CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) genome editing transformation pooled guide RNAs (gRNAs). total, 84 T0 plants representing 40 independent transgenic events harboring mutation each 10 were generated. The average efficiency 18 transformed gRNAs is 75%, ranging from 0 (3 gRNAs) 100% (8 gRNAs). Most diverse range mutations, small indels (1-44 bp) substitutions, which expected impair biogenesis miR482. All nine mutant populations used Verticillium dahliae infection experiments showed index lower than control, with four being significantly lower. assay also suggests role potential dosage effect miR482l. study demonstrates feasibility saturation mutagenesis plant miRNA families dozens genetic loci provides community valuable resource for uncovering miR482-NLR module(s) underlying interaction pathogens.

Language: Английский

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Genome-Wide Identification and Characterization of the CC-NBS-LRR Gene Family in Cucumber (Cucumis sativus L.)

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(9), P. 5048 - 5048

Published: May 2, 2022

The NBS-LRR (NLR) gene family plays a pivotal role in regulating disease defense response plants. Cucumber is one of the most important vegetable crops world, and various plant diseases, including powdery mildew (PM), cause severe losses both cucumber productivity quality annually. To characterize understand CC-NBS-LRR(CNL) genes plants, we performed bioinformatical analysis to these systematically. We identified 33 members CNL they are distributed on each chromosome with 4 harboring largest cluster five different genes. corresponding member varies number amino acids exons, molecular weight, theoretical isoelectric point (pI) subcellular localization. Cis-acting element reveals presence multiple phytohormone, abiotic biotic responsive elements their promoters, suggesting that might be hormones stress. Phylogenetic synteny indicated proteins conserved evolutionarily species, can divided into four subfamilies based domains. MEME sequence alignment showed motifs exist CNLs. Further DNA suggests CsCNL subject regulation miRNAs upon PM infection. By mining available RNA-seq data followed by real-time quantitative PCR (qRT-PCR) analysis, characterized expression patterns genes, found those exhibit temporospatial pattern, also infection, ethylene, salicylic acid, methyl jasmonate treatment Finally, targeted were predicted Our results this study provided some basic information for further functions

Language: Английский

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Low background self-primer EXPAR coupled with colorimetric and lateral flow assay for rapid and sensitive point-of-care detection of miRNA

Sensors and Actuators B Chemical, Journal Year: 2023, Volume and Issue: 399, P. 134856 - 134856

Published: Oct. 26, 2023

Language: Английский

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sRNAminer: A multifunctional toolkit for next-generation sequencing small RNA data mining in plants

Science Bulletin, Journal Year: 2023, Volume and Issue: 69(6), P. 784 - 791

Published: Dec. 29, 2023

Language: Английский

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