From synthetic communities to synthetic ecosystems: exploring causalities in plant–microbe–environment interactions DOI
Guillaume Chesneau, Johannes B. Herpell, Rubén Garrido‐Oter

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Summary The plant microbiota research field has rapidly shifted from efforts aimed at gaining a descriptive understanding of composition to focus on acquiring mechanistic insights into functions and assembly rules. This evolution was driven by our ability establish comprehensive collections plant‐associated microbes reconstruct meaningful microbial synthetic communities (SynComs). We argue that this powerful deconstruction–reconstruction strategy can be used reconstitute increasingly complex ecosystems (SynEcos) mechanistically understand high‐level biological organization. transitioning simple more advanced, fully tractable programmable gnotobiotic SynEcos is ongoing aims rationally simplifying natural engineering them. Such reconstitution ecology approaches represent an untapped for bridging the gap between functional biology unraveling plant–microbiota–environment mechanisms modulate ecosystem health, assembly, functioning.

Language: Английский

From seed to seed: the role of microbial inheritance in the assembly of the plant microbiome DOI Creative Commons
Ahmed Abdelfattah, Ayco J. M. Tack, Carolina Lobato

et al.

Trends in Microbiology, Journal Year: 2022, Volume and Issue: 31(4), P. 346 - 355

Published: Dec. 5, 2022

Despite evidence that the microbiome extends host genetic and phenotypic traits, information on how is transmitted maintained across generations remains fragmented. For seed-bearing plants, seeds harbor a distinct play unique role by linking one generation to next. Studies microbial inheritance, process we suggest including both vertical transmission subsequent migration of seed microorganisms new plant, thus become essential for our understanding evolutionary potential host–microbiome coevolution. We propose dividing inheritance into three stages: (i) plant seed, (ii) dormancy, (iii) seedling. discuss factors affecting assembly during stages, highlight future research directions, emphasize implications fundamental science society.

Language: Английский

Citations

94
Insights into the seed microbiome and its ecological significance in plant life DOI Creative Commons

Aadil Farooq War,

Iqra Bashir, Zafar A. Reshi

et al.

Microbiological Research, Journal Year: 2023, Volume and Issue: 269, P. 127318 - 127318

Published: Feb. 2, 2023

Language: Английский

Citations

28
Engineering the Crop Microbiota Through Host Genetics DOI Creative Commons
Carmen Escudero-Martinez, Davide Bulgarelli

Annual Review of Phytopathology, Journal Year: 2023, Volume and Issue: 61(1), P. 257 - 277

Published: May 17, 2023

The microbiota populating the plant–soil continuum defines an untapped resource for sustainable crop production. host plant is a driver taxonomic composition and function of these microbial communities. In this review, we illustrate how genetic determinants have been shaped by domestication diversification. We discuss heritable component recruitment may represent, at least partially, selection functions underpinning growth, development, health their plants magnitude heritability influenced environment. host–microbiota interactions can be treated as external quantitative trait review recent studies associating genetics with microbiota-based traits. also explore results reductionist approaches, including synthetic communities, to establish causal relationships between phenotypes. Lastly, propose strategies integrate manipulation into programs. Although detailed understanding when deployed breeding purposes still lacking, argue that advances in genomics are likely accelerate wider applications plant–microbiota agriculture.

Language: Английский

Citations

26
Seed biopriming for sustainable agriculture and ecosystem restoration DOI Creative Commons
Prachi Singh, Anukool Vaishnav, Hongwei Liu

et al.

Microbial Biotechnology, Journal Year: 2023, Volume and Issue: 16(12), P. 2212 - 2222

Published: July 25, 2023

Abstract The utilization of microbial inoculants in the realm sustainable agricultural and ecosystem restoration has witnessed a surge recent decades. This rise is largely attributed to advancements our understanding plant–microbe interactions, urgency reduce dependence on agrochemicals growing societal demand for strategies management. However, despite rapid growth bio‐inoculants sector, certain limitations persist concerning their efficacy performance under field condition. Here, we propose that seed biopriming, an effective inoculant technique integrating both biological agents (the priming beneficial microbes seeds) physiological aspects (hydration seeds improved metabolically activity), significant potential mitigate these limitations. method increases protection against soil‐borne pathogens soil pollutants, such as salts heavy metals, while promoting germination rate uniformity, leading overall primary productivity health. Furthermore, argue coating can facilitate transgenerational associations microbes, refine plant microbiomes, maintain legacies microflora. review article aims improve biopriming approach potent valuable tool achieving agriculture successful restoration.

Language: Английский

Citations

25
Seedling microbiota engineering using bacterial synthetic community inoculation on seeds DOI Creative Commons
Gontran Arnault,

Coralie Marais,

Anne Préveaux

et al.

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(4)

Published: March 12, 2024

Abstract Synthetic Communities (SynComs) are being developed and tested to manipulate plant microbiota improve health. To date, only few studies proposed the use of SynCom on seed despite its potential for engineering. We presented a simple effective seedling engineering method using inoculation seeds. The was successful wide diversity compositions bacterial strains that representative common bean microbiota. First, this enables modulation composition community size. Then, SynComs strongly outcompeted native potting soil contributed average 80% showed strain abundance main driver an colonization. Also, selection partly involved in colonization capacities since affiliated Enterobacteriaceae Erwiniaceae were good colonizers while Bacillaceae Microbacteriaceae poor colonizers. Additionally, engineered modified recruitment assembly rhizosphere through priority effects. This study shows seeds represents promising approach consequence fitness.

Language: Английский

Citations

13
Shared governance in the plant holobiont and implications for one health DOI Creative Commons
Gabriele Berg, Cristina Dorador, Dilfuza Egamberdieva

et al.

FEMS Microbiology Ecology, Journal Year: 2024, Volume and Issue: 100(3)

Published: Feb. 13, 2024

Abstract The holobiont Holobiont theory is more than 80 years old, while the importance of microbial communities for plant holobionts was already identified by Lorenz Hiltner a century ago. Both concepts are strongly supported results from new field microbiome research. Here, we present ecological and genetic features that underpin principles shared governance between hosts microbes summarize relevance in context global change. Moreover, uncover knowledge gaps arise when integrating broader perspective holobiome as well one planetary health concepts. Action needed to consider interacting at scale, prediction control function improve human environmental outcomes.

Language: Английский

Citations

12
Diversity and Plant Growth-Promoting Activities of Culturable Seed Endophytes in Abies pindrow (Royle ex D. Don) Royle: Their Role in Seed Germination and Seedling Growth DOI
Iflah Rafiq, Zafar A. Reshi, Iqra Bashir

et al.

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(2)

Published: Jan. 13, 2025

Language: Английский

Citations

1
Insights into quinoa endophytes: core bacterial communities reveal high stability to water stress and genotypic variation DOI Creative Commons
Isaac Maestro‐Gaitán, Miguel Redondo‐Nieto, Sara González-Bodí

et al.

Environmental Microbiome, Journal Year: 2025, Volume and Issue: 20(1)

Published: Feb. 3, 2025

Plant endophytes, comprising non-pathogenic bacteria, fungi, and archaea, inhabit various plant parts, including roots, stems, leaves, seeds. These microorganisms play a crucial role in development by enhancing germination, growth, stress resilience. Seed particular, represent the most adapted conserved segment of microbiota, significantly influencing initial stages growth microbial community establishment. This study investigates impact environmental genotypic factors on endophytic communities Chenopodium quinoa Willd. (quinoa), crop notable for its adaptability nutritional value. We aimed to characterize core seeds roots from two distinct genotypes under well-watered (WW) water-deficit (WD) conditions, utilizing soil infusions as inoculants explore potential changes these endophytes. Our findings reveal with exhibiting high degree conservation their microbiome, even between maternal offspring seeds, specific bacterial taxa showing only minor differences. Tissue specificity emerged key factor, maintaining stable community, while exhibited more pronounced shifts, highlighting tissue-dependent patterns enrichment. The results highlight stability varying water conditions across different genotypes, emphasizing tissue shaping associations. suggest that quinoa-associated particularly those may drought Understanding dynamics plant-microbe interactions is vital developing stress-resilient varieties, supporting sustainable agricultural practices, ensuring food security face climate change challenges.

Language: Английский

Citations

1
Lactuchelins: New lipopeptide siderophores from Pseudomonas lactucae inhibit Xanthomonas campestris pv. campestris 8004 DOI Open Access
Guillaume Chesneau,

Alba Noël,

Dimitri Bréard

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Abstract Seeds harbor diverse microbial communities, including beneficial microbes that play a vital role in protecting plants from seed-borne pathogens. Despite their critical importance, the molecular mechanisms driving intermicrobial competition within seed microbiome remain poorly understood, limiting potential to optimize inoculation strategies. In this study, we evaluated inhibitory of 30 bacterial strains against phytopathogen Xanthomonas campestris pv. 8004 (Xcc8004). We identified Pseudomonas lactucae CFBP13502 as potent inhibitor Xcc8004, mediated by exometabolites specifically induced presence Lysobacterales (formerly Xanthomonadales). Transcriptomic analysis revealed upregulation gene cluster involved biosynthesis lipopeptide siderophore biosynthesis. Gene deletion confirmed is essential for growth inhibition Xcc8004. Furthermore, iron supplementation abolished effect, providing strong evidence chelation. Through comparative metabolomics, elucidated structure novel family siderophores, which named lactuchelins, produced CFBP13502. Our findings provide first competitive exclusion at interface, highlighting lactuchelins promising avenue development seed-based biocontrol strategies phytopathogens.

Language: Английский

Citations

1
MetaFlowTrain: a highly parallelized and modular fluidic system for studying exometabolite-mediated inter-organismal interactions DOI Creative Commons
Guillaume Chesneau, Johannes B. Herpell, Sarah Wolf

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 10, 2025

Language: Английский

Citations

1