Comparing native and non-native seed-isolated strains for drought resilience in maize (Zea mays L.)

Plant Stress, Journal Year: 2024, Volume and Issue: 12, P. 100462 - 100462

Published: April 17, 2024

Maize, a vital crop for human nutrition, livestock, and industrial development, faces increasingly severe climatic conditions that hinder its production capacity along with other strategic crops. Novel treatments based on microorganisms have demonstrated efficiency in enhancing plant development responding to stress. The use of bacteria isolated from seeds is novel approach biotreatment, as recent studies point co-evolution process their presence seeds. This hypothesize pre-adaptation host, which may lead increased efficiency. However, several aspects this remain understudied. In study, we aimed evaluate the potential native maize seed microbiota comparison species mitigate drought For characterized seedborne common productive variety Portugal, it biotreatment two varieties (sensitive resistant drought), selecting strain Pseudomonas fulva MB most promising candidate. Stenotrophomonas maltophilia MS-M1 strain, wild alfalfa previously drought-tolerant enhancer, served non-native control strain. . According data, both enhanced vegetative growth when treated individual strains, well consortium, an increase height 5-7% full medium irrigation, 50-55% not irrigated. trend was also observed weight, by 13-23%, even under no irrigation. addition, positively impacted these treatments, amount grain produced (by weight). drought-sensitive experienced 17% while tolerant 25-40% increase. Under irrigation level, about 15% varieties, 100% 140% each variety, respectively, applied. results suggest P. slightly more efficient treatment, outperformed terms productivity varieties. differences were solid enough all parameters consistently asses such difference. consortium treatment only showed better performance or some traits. These findings support very biotreatments, suggesting than strains remarkable beneficial impact (interspecific), expanding possible kind bioinoculants. Further test are required optimize seed-isolated adapted tailor-made solutions agriculture.

Language: Английский

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Determining the footprint of breeding in the seed microbiome of a perennial cereal

Environmental Microbiome, Journal Year: 2024, Volume and Issue: 19(1)

Published: June 17, 2024

Abstract Background Seed endophytes have a significant impact on plant health and fitness. They can be inherited passed to the next generation. However, of breeding their composition in seeds is less understood. Here, we studied indigenous seed microbiome recently domesticated perennial grain crop (Intermediate wheatgrass, Thinopyrum intermedium L.) that promises great potential for harnessing microorganisms enhance performance by multiphasic approach, including amplicon strain libraries, as well molecular physiological assays. Results Intermediate wheatgrass harvested from four field sites Europe over three consecutive years were dominated Proteobacteria (88%), followed Firmicutes (10%). Pantoea was most abundant genus agglomerans identified only core taxon present all samples. While bacterial diversity species richness similar across accessions, relative abundance varied especially terms low rare taxa. Seeds different cycles (TLI C3, C5, C704, C801) showed differences community abundance. We found decrease functional genes nirK nifH drop richness. This associated with loss sequence variants (ASVs) Actinobacteria , Alphaproteobacteria Bacilli which could partially compensated offspring seeds, been cultivated at new site. Interestingly, subset assigned potentially beneficial bacteria, e.g. Pantoea, Kosakonia Pseudomonas transmitted generation or shared seeds. Conclusion Overall, this study advances our understanding assembly transmission endophytic intermediate highlights importance considering future programs.

Language: Английский

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Microbial controls on seed germination

Soil Biology and Biochemistry, Journal Year: 2024, Volume and Issue: 199, P. 109576 - 109576

Published: Sept. 2, 2024

Germination onset is the first stage in phenological plant cycle, influenced by abiotic and biotic factors. Both soil seed microbiota are key drivers of germination, influencing storage, dormancy release, germination rates. Interactions between plants microbes contribute to adaptation their environment. Therefore, could benefit more from interacting with local ('home') environment than those other origins. As crucial drivers, may select for specific microbial taxa that provide them a home-field advantage, regardless richness diversity surroundings. Here, we looked at role seed-associated microorganisms on holm oak (Quercus ilex) whether or have greater impact this process, how interaction microbiotas influence germination. We found Q. ilex seeds significant effect non-sterilised having higher germinated acorns sterilised ones. Moreover, when co-occurring, enhance Overall, our results evidence advantage where communities, along microorganisms, over different species, evidencing importance fitness.

Language: Английский

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The chemical language of plant–microbe–microbe associations: an introduction to a Virtual Issue

New Phytologist, Journal Year: 2024, Volume and Issue: 244(3), P. 739 - 742

Published: Oct. 2, 2024

This Editorial introduces the Virtual Issue ‘Chemical language of plant–microbe–microbe associations’ that includes following papers: Basak et al . (2024), Böttner (2023), Brisson Feng Gfeller Gómez‐Pérez Hong (2022, 2023), Hu Jiang Lee Nakano Ökmen Revillini Rovenich & Thomma Simonin (2022), Snelders Walsh Wen Xia Xie Zhang (2023, 2024), Zheng Zhou 2024). Access at www.newphytologist.com/virtualissues

Language: Английский

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From synthetic communities to synthetic ecosystems: exploring causalities in plant–microbe–environment interactions

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Summary The plant microbiota research field has rapidly shifted from efforts aimed at gaining a descriptive understanding of composition to focus on acquiring mechanistic insights into functions and assembly rules. This evolution was driven by our ability establish comprehensive collections plant‐associated microbes reconstruct meaningful microbial synthetic communities (SynComs). We argue that this powerful deconstruction–reconstruction strategy can be used reconstitute increasingly complex ecosystems (SynEcos) mechanistically understand high‐level biological organization. transitioning simple more advanced, fully tractable programmable gnotobiotic SynEcos is ongoing aims rationally simplifying natural engineering them. Such reconstitution ecology approaches represent an untapped for bridging the gap between functional biology unraveling plant–microbiota–environment mechanisms modulate ecosystem health, assembly, functioning.

Language: Английский

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