Genomic analyses elucidate the causes and consequences of breakdown of distyly inLinum trigynum DOI Creative Commons
Juanita Gutiérrez‐Valencia, Panagiotis‐Ioannis Zervakis, Zoé Postel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 17, 2023

Abstract Distyly is an iconic floral polymorphism governed by a supergene, which promotes efficient pollen transfer and outcrossing through reciprocal differences in the position of sexual organs flowers, often coupled with heteromorphic self-incompatibility (SI). has evolved convergently multiple flowering plant lineages, but also broken down repeatedly, resulting homostylous, self-compatible populations elevated rates self-fertilization. Here, we aimed to study genetic causes genomic consequences shift homostyly Linum trigynum , closely related distylous tenue. Building on high-quality genome assembly, show that L. harbors region homologous dominant haplotype distyly supergene conferring long stamens short styles tenue suggesting loss first occurred short-styled individual. In contrast homostylous Primula Fagopyrum no fixed loss-of-function mutations coding sequences S- linked candidate genes. Instead, gene expression analyses controlled crosses suggest downregulating LtWDR-44 for male SI and/or anther height could underlie self-compatibility (SC) . Population 224 whole-genome further demonstrate highly self-fertilizing, exhibits significantly lower diversity genome-wide, experiencing relaxed purifying selection less frequent positive nonsynonymous relative Our shed light advance our understanding common evolutionary transition plants.

Language: Английский

Phosphoproteomic analysis of distylous Turnera subulata identifies pathways related to endoreduplication that correlate with reciprocal herkogamy DOI Creative Commons
Paige M. Henning, Benjamin B. Minkoff, Michael R. Sussman

et al.

American Journal of Botany, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 17, 2024

A multi-omic approach was used to explore proteins and networks hypothetically important for establishing filament dimorphisms in heterostylous Turnera subulata (Sm.) as an exploratory method identify genes future empirical research.

Language: Английский

Citations

0
Cell elongation and altered phytohormone levels play a role in establishing distyly in Averrhoa carambola DOI

Wubaiyu Lin,

Qin Si,

Siyu Chen

et al.

Gene, Journal Year: 2024, Volume and Issue: 939, P. 149167 - 149167

Published: Dec. 15, 2024

Language: Английский

Citations

0
Genomic analyses elucidate S-locus evolution in response to intra-specific losses of distyly Primula vulgaris DOI Open Access
Emiliano Mora‐Carrera, Rebecca L. Stubbs, Giacomo Potente

et al.

Authorea (Authorea), Journal Year: 2023, Volume and Issue: unknown

Published: July 21, 2023

Distyly, a floral dimorphism that promotes outcrossing, is controlled by hemizygous genomic region known as the S-locus. Disruptions of genes within S-locus are responsible for loss distyly and emergence homostyly, monomorphism favors selfing. Using whole genome resequencing data distylous homostylous individuals from populations Primula vulgaris leveraging high-quality reference genomes we tested, first time, predictions about evolutionary consequences transitions to selfing on genes. Our results confirm presence previously reported homostyle-specific, loss-of-function mutations in exons gene CYPᵀ , while also revealing undetected structural rearrangement associated with shift homostyly. Additionally, discovered promoter identical, suggesting down-regulation via its not cause Furthermore, found hemizygosity leads reduced genetic diversity less efficient purifying selection compared outside S-locus, homostyly further lowers diversity, expected mating-system shifts. Finally, long-standing theoretical models changes genotypes during early stages transition supporting assumption two (diploid) copies might reduce homostyle viability.

Language: Английский

Citations

1
ThePrimula edelbergii S-locus is an example of a jumping supergene DOI Open Access
Giacomo Potente, Narjes Yousefi, Barbara Keller

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 31, 2024

Abstract Research on supergenes, non-recombining genomic regions housing tightly linked genes that control complex phenotypes, has gained prominence in genomics, with supergenes having been described most eukaryotic lineages. Heterostyly, a floral heteromorphism promoting outcrossing several angiosperm families, is controlled by the S -locus supergene. Historically, studied primarily closely related Primula species and, more recently, other groups independently evolved heterostyly. However, it remains unknown whether genetic architecture and composition of are maintained among share common origin heterostyly subsequently diverged across larger time scales. To address this research gap, we present chromosome-scale genome assembly edelbergii , shares same veris (whose characterized) but from ca. 18 million years ago. Comparative analyses between P. allowed us to show, for first time, can ‘jump’ (i.e. translocate) chromosomes. Additionally, found four were reshuffled within supergene, seemingly without affecting their expression. Furthermore, confirmed hemizygosity counteracts degeneration, otherwise expected supergenes. Finally, investigated evolutionary history Ericales terms whole duplications transposable element accumulation. In summary, our work provides valuable resource comparative aimed at investigating genetics pivotal role shaping evolution phenotypes.

Language: Английский

Citations

0
Genomic analyses elucidate the causes and consequences of breakdown of distyly inLinum trigynum DOI Creative Commons
Juanita Gutiérrez‐Valencia, Panagiotis‐Ioannis Zervakis, Zoé Postel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 17, 2023

Abstract Distyly is an iconic floral polymorphism governed by a supergene, which promotes efficient pollen transfer and outcrossing through reciprocal differences in the position of sexual organs flowers, often coupled with heteromorphic self-incompatibility (SI). has evolved convergently multiple flowering plant lineages, but also broken down repeatedly, resulting homostylous, self-compatible populations elevated rates self-fertilization. Here, we aimed to study genetic causes genomic consequences shift homostyly Linum trigynum , closely related distylous tenue. Building on high-quality genome assembly, show that L. harbors region homologous dominant haplotype distyly supergene conferring long stamens short styles tenue suggesting loss first occurred short-styled individual. In contrast homostylous Primula Fagopyrum no fixed loss-of-function mutations coding sequences S- linked candidate genes. Instead, gene expression analyses controlled crosses suggest downregulating LtWDR-44 for male SI and/or anther height could underlie self-compatibility (SC) . Population 224 whole-genome further demonstrate highly self-fertilizing, exhibits significantly lower diversity genome-wide, experiencing relaxed purifying selection less frequent positive nonsynonymous relative Our shed light advance our understanding common evolutionary transition plants.

Language: Английский

Citations

0