Plant Communications, Journal Year: 2024, Volume and Issue: 6(1), P. 101137 - 101137
Published: Sept. 23, 2024
Language: Английский
The Plant Journal, Journal Year: 2024, Volume and Issue: 119(2), P. 927 - 941
Published: June 14, 2024
Acteoside is a bioactive phenylethanoid glycoside widely distributed throughout the plant kingdom. Because of its two catechol moieties, acteoside displays variety beneficial activities. The biosynthetic pathway has been largely elucidated, but assembly logic moieties in remains unclear. Here, we identified novel polyphenol oxidase OfPPO2 from Osmanthus fragrans, which could hydroxylate various monophenolic substrates, including tyrosine, tyrosol, tyramine, 4-hydroxyphenylacetaldehyde, salidroside, and osmanthuside A, leading to formation corresponding catechol-containing intermediates for biosynthesis. also convert B into acteoside, creating directly via post-modification skeleton. reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis subcellular localization assay further support involvement biosynthesis planta. These findings suggest that O. fragrans may follow "parallel routes" rather than conventionally considered linear route. In this hypothesis, glycosyltransferase OfUGT acyltransferase OfAT direct flux diphenolic generated by acteoside. Significantly, orthologs constitute functionally conserved enzyme family evolved independently other known enzymes implying substrate promiscuity PPO offer acteoside-producing plants alternative ways synthesize Overall, work expands our understanding parallel pathways employ efficiently strategy contribute plants' adaptation environmental challenges.
Language: Английский
Citations
4
Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(11), P. 4116 - 4134
Published: June 23, 2024
Emerald ash borer (EAB, Agrilus planipennis) is an invasive killer of trees (Fraxinus spp.) in North America and Europe. Ash species co-evolved with EAB their native range Asia are mostly resistant, although the precise mechanism(s) remain unclear. Very little also known about or tree microbiomes. We performed first joint comparison phloem mycobiome metabolites between a nonnative species, infested uninfested EAB, conjunction investigation larval mycobiome. Phloem communities differed but both were unaffected by infestation. Several indicator taxa gut shared similarly high relative abundance only host trees. Widely targeted metabolomics revealed 24 distinct 53 trees, respectively, that content one species. Interestingly, four strong relationship mycobiomes, majority which affected Collectively, our results demonstrate complex interplay chemistry mycobiome, suggest relationships mycobiomes may reflect co-evolution.
Language: Английский
Citations
4
Journal of Applied Ecology, Journal Year: 2025, Volume and Issue: unknown
Published: March 26, 2025
Abstract Artificial light at night (ALAN) is a recognized threat not only to urban areas but also more remote habitats including forests. Advancements in LED technology offer potential for streetlight infrastructure with minimized disruptive impact on ecosystems. Light dimming ranges among the most promising ALAN‐mitigation measures. Despite being essential understanding ALAN effects ecosystems, little known about how may affect bottom‐up interactions, such as those between plants and herbivores. We conducted forest‐located experiment potted European beech saplings ( Fagus sylvatica L.) that lasted 43 nights. studied of different intensities i.e. 70 lx (100% intensity), 35 (50% 21 (30% intensity) dark control leaf morphological chemical traits, insect herbivory links light‐induced changes traits herbivory. The 100% 50% intensity treatments caused an increase average area reduced specific area. induced specialised metabolome, mainly affecting flavonols. Responses individual metabolites were highly variable, many already responding 30% intensity. Insect was under reduction correlated ALAN‐related phytochemistry. Synthesis applications : Our findings indicate reducing (dimming) step towards sustainable outdoor lighting mitigate tree‐herbivore interactions. However, physiology, phytochemistry detectable even high levels. varying responses across trophic levels highlight challenge optimizing parameters order minimize impacts species To address this challenge, our study advocates increased collaboration ecologists engineers.
Language: Английский
Citations
0
Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown
Published: April 15, 2025
ABSTRACT Plants have coevolved with herbivorous insects for millions of years, resulting in variation resistance both within and between species. Using a manipulative experiment combined untargeted metabolomics, microbiome sequencing transcriptomics approaches, we investigated the roles plant metabolites defence mechanisms native resistant Manchurian ash ( Fraxinus mandshurica ) trees non‐native susceptible velvet velutina against highly invasive emerald borer (EAB, Agrilus planipennis ). Comparative metabolomics analyses show that phenylpropanoid pathway, which is enriched differentially expressed genes abundant metabolites, may serve as potential regulator resistance. Additionally, distinctly shifted two Indicator taxa analysis reveals distinct genera are dominant galleries species, example, Pseudomonas velvet, Hafnia‐Obesumbacterium Manchurian. The strong correlation indicator suggests chemical compounds might impact microbial community phloem directly or indirectly, vice versa. This study significantly enhances our understanding species its contribution to invasion success EAB, providing valuable insights development pest management strategies.
Language: Английский
Citations
0
Total Environment Advances, Journal Year: 2025, Volume and Issue: unknown, P. 200125 - 200125
Published: April 1, 2025
Language: Английский
Citations
0
Environmental Entomology, Journal Year: 2025, Volume and Issue: unknown
Published: May 11, 2025
Abstract Four North American and one Asian ash species were planted in 2007 30 complete randomized blocks a common garden Ingham County, Michigan USA to evaluate host resistance preference of emerald borer (EAB) (Agrilus planipennis Fairmaire), first detected this area 2003. Trees protected from EAB colonization until 2012. We recorded current-year woodpecker holes adult exits on live trees annually 2017 2022. Annual radial growth was quantified increment cores cross-sections EAB-killed trees. Every Fraxinus nigra killed by 2013. By August 2022, 63% F. pennsylvanica, 12% americana 86% chinensis had died. In contrast, quadrangulata minimally colonized remained healthy through Average (± SE) annual 2007-2021 ranged 2.65 ± 0.18 mm for 4.61 0.46 chinensis. an adjacent plantation 2010, we assessed size, signs 2022 12 pennsylvanica mandshurica. All mandshurica with little evidence injury. Despite heavy infestation, 2011 relatively high. Results show is highly preferred vulnerable EAB, followed while intermediate resistant. Of the 2 species, resistant but heavily most
Language: Английский
Citations
0
New Phytologist, Journal Year: 2024, Volume and Issue: 242(6), P. 2495 - 2509
Published: April 19, 2024
Summary Extreme droughts can have long‐lasting effects on forest community dynamics and species interactions. Yet, our understanding of how drought legacy modulates ecological relationships is just unfolding. We tested the hypothesis that leaf chemistry herbivory show long‐term responses to premature defoliation caused by an extreme event in European beech ( Fagus sylvatica L.). For two consecutive years after summer 2018, we collected leaves from upper lower canopy adjacently growing drought‐stressed unstressed trees. Leaf was analyzed damage different herbivore‐feeding guilds quantified. found had lasting impacts nutrients specialized metabolomic profiles. However, did not affect primary metabolome. Drought‐related phytochemical changes affected leaf‐chewing herbivores whereas other largely unaffected. Drought phytochemistry were often weaker than between‐year or between‐canopy strata variability. Our findings suggest a single bears potential long‐lastingly tree–herbivore likely become more important modulating interactions since frequency severity are projected globally increase coming decades.
Language: Английский
Citations
2
Current Research in Insect Science, Journal Year: 2024, Volume and Issue: 7, P. 100105 - 100105
Published: Dec. 31, 2024
Language: Английский
Citations
2
Forestry An International Journal of Forest Research, Journal Year: 2024, Volume and Issue: 97(5), P. 678 - 691
Published: Aug. 6, 2024
Abstract Hymenoscyphus fraxineus is an introduced ascomycete fungus which causes ash dieback and has resulted in widespread mortality of throughout Europe. Although H. been present on the continent for at least four decades, it was not identified until 2006. The first record pathogen Britain came 2012 although probably a decade earlier. most common host European (Fraxinus excelsior L.) economically ecologically important to where cost estimated billions pounds. impact stimulated major research response we review with aim providing up-to-date information relevant identifying knowledge gaps would contribute improved disease mitigation. outcrossing high genotypic diversity; ascospores produced via sexual reproduction are critical aerial dispersal infection. Temperature, moisture, ground cover influence fruit body development, timing ascospore release, extent germination; they also interact together affect likelihood In addition, stand characteristics, including tree density, height, landscape fragmentation, dynamics increased severity moist sites density. Efforts finding natural resistance have genetic markers associated tolerance, gene expression analysis insights into basis that resistance. Mainland findings indicate makes trees more vulnerable other pathogens, whilst endophytes phyllosphere can suppress infection by fraxineus. Possible tools long-term control include (1) deployment resistant ash, (2) quantitatively informed management practices based microclimate models better understanding life cycle, (3) manipulation biocontrol agents from microbiome or mycoviruses.
Language: Английский
Citations
1
Urban forestry & urban greening, Journal Year: 2024, Volume and Issue: 101, P. 128529 - 128529
Published: Oct. 5, 2024
Language: Английский
Citations
1