The antisense CircRNAVvcircABH controls salt tolerance and the brassinosteroid signaling response by suppressing cognate mRNA splicing in grape DOI
Zhen Gao,

Yifan Su,

Yaru Wang

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Summary Soil salinization is a major factor limiting the sustainable development of grape industry. Circular RNAs (circRNAs) are more stable than linear mRNAs and involved in stress responses. However, biological functions molecular mechanisms underlying antisense circRNAs plants remain unclear. We identified circRNA VvcircABH through high‐throughput sequencing. Using genetic transformation methods techniques, we analyzed effects on response to salt its effects. was located nucleus upregulated by stress, while expression level cognate gene VvABH (alpha/beta‐hydrolase) downregulated. overexpression or silencing greatly enhanced tolerance. could bind overlapping region inhibits pre‐mRNA splicing, thereby decreasing . Additionally, repressed additive effect interaction between VvBRI1 (brassinosteroid‐insensitive 1) VvBKI1 (BRI1 kinase inhibitor 1), thus influencing plant's BR, which plays important roles plant conclude that play distinct tolerance brassinosteroid signaling response, govern inhibiting splicing.

Language: Английский

Ethylene increases the NaHCO3 stress tolerance of grapevines partially via the VvERF1B‐VvMYC2‐VvPMA10 pathway DOI Creative Commons

Guangqing Xiang,

Zongbao Fan,

Shuxia Lan

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 7, 2025

Summary Here, we evaluated the role of ethylene in regulating NaHCO 3 stress tolerance grapevines and clarified mechanism by which VvERF1B regulates response to stress. The exogenous application ACC VvACS3 overexpression grape calli revealed that increased tolerance, this was accompanied plasma membrane H + ‐ATPase (PMA) activity. expression strongly induced ACC, gene conferred enhanced PMA activity oxalate secretion. Additionally, function also verified using mutant transgenic Arabidopsis plants. VvPMA10 following grapevine roots, shown regulate activity, secretion, via its mutation calli, and/or . However, not a direct target but directly transactivated VvMYC2. VvMYC2 be similar calli. Additional experiments interaction with ability activate played VvERF1B‐mediated pathway. Overall, VvERF1B‐VvMYC2‐VvPMA pathway ethylene‐induced grapevines, process contributed increases

Language: Английский

Citations

1
Whole transcriptome sequencing-based identification and functional prediction of salt-tolerant-related circular RNAs in ZM-4 (Malus zumi) DOI
Zhao Liu, Xiang Lu,

Hanxin Guo

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141572 - 141572

Published: March 5, 2025

Language: Английский

Citations

0
VvD14c-VvMAX2-VvLOB/VvLBD19 module is involved in the strigolactone-mediated regulation of grapevine root architecture DOI Creative Commons
Yan Xu,

Zhengxin Lv,

Muhammad Aamir Manzoor

et al.

Molecular Horticulture, Journal Year: 2024, Volume and Issue: 4(1)

Published: Oct. 25, 2024

Abstract The D14 protein, an alpha/beta hydrolase, is a key receptor in the strigolactone (SL) signaling pathway. However, response of VvD14 to SL signals and its role grapevine root architecture formation remain unclear. This study demonstrated that VvD14c was highly expressed tissues fruit stages than other isoforms. Application GR24, analog, enhanced elongation diameter adventitious roots but inhibited density lateral (LRs) increased expression. Additionally, GR24 nested within pocket strongly bound with affinity 5.65 × 10 −9 M. Furthermore, interacted MORE AXILLARY GROWTH 2 (VvMAX2) GR24-dependent manner. Overexpression d14 mutant VvMAX2 max2 Arabidopsis reversed LR number density, as well primary elongation. Conversely, homologous overexpression resulted reduced grapevines. directly LATERAL ORGAN BOUNDARY (VvLOB) VvLBD19, thereby positively regulating density. These findings highlight SLs architecture, potentially via VvD14c-VvMAX2-VvLOB/VvLBD19 module, providing new insights into regulation growth development

Language: Английский

Citations

1
The bioinformatic tools, characteristics, biological functions and molecular mechanisms associated with plant circular RNA DOI Creative Commons
Xiaowen Han, Yan Li,

Wai Kyaw Htet Wai

et al.

New Crops, Journal Year: 2024, Volume and Issue: 2, P. 100062 - 100062

Published: Nov. 29, 2024

Language: Английский

Citations

1
Os-circANK negatively regulates resistance to bacterial blight in rice via Osa-miR398b/OsCSD1/OsCSD2 pathways DOI Creative Commons
Xiaohui Liu, Peihong Wang,

Sai Wang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 18, 2024

Abstract CircRNAs are widely present in plants, yet there have been no reports on the role of circRNA regulatory mechanisms interactions between rice and pathogenic bacteria. As sequence circRNAs matches parent gene except for back splicing site, is feasible way to specifically knockdown plants. We identified a circRNA, named Os-circANK, derived from an ankyrin repeat protein, which was significantly downregulated post Xanthomonas oryzae pv. ( Xoo ) inoculation. created CRISPR-Cas13d system plant circular RNA, resulting genetically edited with targeted decrease Os-circANK expression. found function as sponge Osa-miR398b, suppressing cleavage OsCSD1/OsCSD2 by leading reduction ROS levels following infection negative regulation resistance bacterial blight rice. Our findings demonstrate that inhibits via Osa-miR398b/OsCSD1/OsCSD2 pathways.

Language: Английский

Citations

0
Investigation of grapevine circular RNA revealed the function on root development and salt stress resistance DOI
Jingjing Liu, Yi Ren, Yuanyuan Xu

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 337, P. 113489 - 113489

Published: July 24, 2024

Language: Английский

Citations

0
The antisense CircRNAVvcircABH controls salt tolerance and the brassinosteroid signaling response by suppressing cognate mRNA splicing in grape DOI
Zhen Gao,

Yifan Su,

Yaru Wang

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Summary Soil salinization is a major factor limiting the sustainable development of grape industry. Circular RNAs (circRNAs) are more stable than linear mRNAs and involved in stress responses. However, biological functions molecular mechanisms underlying antisense circRNAs plants remain unclear. We identified circRNA VvcircABH through high‐throughput sequencing. Using genetic transformation methods techniques, we analyzed effects on response to salt its effects. was located nucleus upregulated by stress, while expression level cognate gene VvABH (alpha/beta‐hydrolase) downregulated. overexpression or silencing greatly enhanced tolerance. could bind overlapping region inhibits pre‐mRNA splicing, thereby decreasing . Additionally, repressed additive effect interaction between VvBRI1 (brassinosteroid‐insensitive 1) VvBKI1 (BRI1 kinase inhibitor 1), thus influencing plant's BR, which plays important roles plant conclude that play distinct tolerance brassinosteroid signaling response, govern inhibiting splicing.

Language: Английский

Citations

0