Deterministic colonization arises early during the transition of soil bacteria to the phyllosphere and is shaped by plant-microbe interactions. DOI Creative Commons
Teresa Mayer,

Erik Teutloff,

Kerstin Unger

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Background Upon seed germination, soil bacteria are activated to transition the plant and eventually colonize mature tissues like leaves. These poised significantly influence health, but we know little about their colonization routes. We studied mechanisms of germinating plants leaves by experimentally manipulating inoculation times using in-planta isolation understand that can make complex soil-to-leaf transition. Using a trackable, labeled Pseudomonas viridiflava ( Pv 3D9) amended soil, tested how plant-microbe-microbe interactions shape assembly in natural communites. Results found stages from before true leaf emergence were important establishing uniquely diverse bacteriomes. A wide diversity leaf-associated taxa individually this transition, most still shaped stochastic processes. Interestingly, deterministic processes drove some only when they transitioned leaves, not inoculated later. The opportunistic pathogen 3D9 promoted growth likely activating immunity. effects turn strongly affected almost strictly colonized deterministically, demonstrating role controlling Conclusions Diverse, well-adapted bacterial during resulting characteristic healthy microbiomes. domination indicates many must compete establish niche. During however, specific emerge driven processes, suggesting occupy unique niches. malleability these suggests may be controlled, for example targeted manipulations. This finding is significant given roles health offers directions forward engineering beneficial

Language: Английский

A phage tail–like bacteriocin suppresses competitors in metapopulations of pathogenic bacteria DOI

Talia Backman,

Sergio M. Latorre, Efthymia Symeonidi

et al.

Science, Journal Year: 2024, Volume and Issue: 384(6701)

Published: June 13, 2024

Bacteria can repurpose their own bacteriophage viruses (phage) to kill competing bacteria. Phage-derived elements are frequently strain specific in killing activity, although there is limited evidence that this specificity drives bacterial population dynamics. Here, we identified intact phage and derived a metapopulation of wild plant-associated Pseudomonas genomes. We discovered the most abundant viral cluster encodes remnant resembling tail called tailocin, which bacteria have co-opted competitors. Each pathogenic carries one few distinct tailocin variants target variable polysaccharides outer membrane co-occurring strains. Analysis herbarium samples from past 170 years revealed same receptor persisted populations. These results suggest genetic diversity be mined develop targeted "tailocin cocktails" for microbial control.

Language: Английский

Citations

12
Deterministic colonization arises early during the transition of soil bacteria to the phyllosphere and is shaped by plant–microbe interactions DOI Creative Commons
Teresa Mayer,

Erik Teutloff,

Kerstin Unger

et al.

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: April 22, 2025

Language: Английский

Citations

0
Application of inhibitors targeting the type III secretion system in phytopathogenic bacteria DOI

Lu-Lu He,

Lan-Tu Xiong,

Xin Wang

et al.

Chinese Chemical Letters, Journal Year: 2024, Volume and Issue: unknown, P. 110044 - 110044

Published: May 1, 2024

Language: Английский

Citations

1
Continental-scale associations of Arabidopsis thaliana phyllosphere members with host genotype and drought DOI Creative Commons
Talia L. Karasov, Manuela Neumann, Laura Leventhal

et al.

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(10), P. 2748 - 2758

Published: Sept. 6, 2024

Abstract Plants are colonized by distinct pathogenic and commensal microbiomes across different regions of the globe, but factors driving their geographic variation largely unknown. Here, using 16S ribosomal DNA shotgun sequencing, we characterized associations Arabidopsis thaliana leaf microbiome with host genetics climate variables from 267 populations in species’ native range Europe. Comparing distribution 575 major bacterial amplicon variants (phylotypes), discovered that composition A. segregates along a latitudinal gradient. The clines predicted metrics drought, also spatial host. To validate relative effects drought genotype conducted common garden field study, finding 10% core bacteria to be affected directly 20% genetic drought. These data provide valuable resource for plant field, identified suggesting can indirectly shape via microbiome.

Language: Английский

Citations

1
Genetic Mapping of Tolerance to Bacterial Stem Blight Caused by Pseudomonas syringae pv. syringae in Alfalfa (Medicago sativa L.) DOI Creative Commons

Yeidymar Sierra Moya,

Cesar Augusto Medina,

Bianca Herrera

et al.

Plants, Journal Year: 2023, Volume and Issue: 13(1), P. 110 - 110

Published: Dec. 29, 2023

The bacterial stem blight of alfalfa (Medicago sativa L.), first reported in the United States 1904, has emerged recently as a serious disease problem western states. causal agent, Pseudomonas syringae pv. syringae, promotes frost damage and that can reduce harvest yields by 50%. Resistant cultivars an understanding host-pathogen interactions are lacking this pathosystem. With goal identifying DNA markers associated with resistance, we developed biparental F1 mapping populations using plants from cultivar ZG9830. Leaflets were inoculated suspension needleless syringe scored for symptoms. Bacterial measured culture plating quantitative PCR assay. Surprisingly, leaflets few to no symptoms had loads similar severe symptoms, indicating without tolerant bacterium. Genotyping-by-sequencing identified 11 significant SNP tolerance phenotype. This is study identify P. syringae. These results provide insight into host responses be used breeding programs develop improved manage alfalfa.

Language: Английский

Citations

2
Deterministic colonization arises early during the transition of soil bacteria to the phyllosphere and is shaped by plant-microbe interactions. DOI Creative Commons
Teresa Mayer,

Erik Teutloff,

Kerstin Unger

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Background Upon seed germination, soil bacteria are activated to transition the plant and eventually colonize mature tissues like leaves. These poised significantly influence health, but we know little about their colonization routes. We studied mechanisms of germinating plants leaves by experimentally manipulating inoculation times using in-planta isolation understand that can make complex soil-to-leaf transition. Using a trackable, labeled Pseudomonas viridiflava ( Pv 3D9) amended soil, tested how plant-microbe-microbe interactions shape assembly in natural communites. Results found stages from before true leaf emergence were important establishing uniquely diverse bacteriomes. A wide diversity leaf-associated taxa individually this transition, most still shaped stochastic processes. Interestingly, deterministic processes drove some only when they transitioned leaves, not inoculated later. The opportunistic pathogen 3D9 promoted growth likely activating immunity. effects turn strongly affected almost strictly colonized deterministically, demonstrating role controlling Conclusions Diverse, well-adapted bacterial during resulting characteristic healthy microbiomes. domination indicates many must compete establish niche. During however, specific emerge driven processes, suggesting occupy unique niches. malleability these suggests may be controlled, for example targeted manipulations. This finding is significant given roles health offers directions forward engineering beneficial

Language: Английский

Citations

0