Complementary environmental analysis and functional characterization of a plastid diatom lower glycolytic-gluconeogenesis pathway DOI Creative Commons
Richard G. Dorrell, Youjun Zhang, Yue Liang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 9, 2022

Abstract Organic carbon fixed in chloroplasts through the Calvin Cycle can be diverted towards different metabolic fates, including cytoplasmic and mitochondrial respiration; gluconeogenesis; synthesis of diverse plastid metabolites via pyruvate hub. In plants, is principally produced glycolysis, although a plastid-targeted lower glycolytic pathway known non-photosynthetic tissue. Here, we characterize glycolytic-gluconeogenesis diatoms, ecologically important marine algae distantly related to plants. We show that two reversible enzymes required complete diatom glycolysis-gluconeogenesis, Enolase PGAM ( bis- phospho-glycerate mutase), originated duplications mitochondria-targeted respiratory isoforms. Through CRISPR-Cas9 mutagenesis, integrative ‘omic analyses, measured kinetics expressed Phaeodactylum tricornutum , present evidence this diverts glyceraldehyde-3-phosphate into hub, may also function gluconeogenic direction. Considering experimental data, has roles dependent particular on day length environmental temperature, it at elevated levels high latitude oceans where diatoms are abundant. Our data provide evolutionary, meta-genomic functional insights poorly understood yet evolutionarily recurrent pathway.

Language: Английский

Complementary environmental analysis and functional characterization of lower glycolysis-gluconeogenesis in the diatom plastid DOI Creative Commons
Richard G. Dorrell, Youjun Zhang, Yue Liang

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3584 - 3610

Published: June 6, 2024

Organic carbon fixed in chloroplasts through the Calvin-Benson-Bassham Cycle can be diverted toward different metabolic fates, including cytoplasmic and mitochondrial respiration, gluconeogenesis, synthesis of diverse plastid metabolites via pyruvate hub. In plants, is principally produced glycolysis, although a plastid-targeted lower glycolytic pathway known to exist non-photosynthetic tissue. Here, we characterized glycolysis-gluconeogenesis enabling direct interconversion glyceraldehyde-3-phosphate phospho-enol-pyruvate diatoms, ecologically important marine algae distantly related plants. We show that two reversible enzymes required complete diatom glycolysis-gluconeogenesis, Enolase bis-phosphoglycerate mutase (PGAM), originated duplications mitochondria-targeted respiratory isoforms. Through CRISPR-Cas9 mutagenesis, integrative 'omic analyses, measured kinetics expressed Phaeodactylum tricornutum, present evidence this diverts into hub, may also function gluconeogenic direction. Considering experimental data, has roles dependent particular on day length environmental temperature, cpEnolase cpPGAM genes are at elevated levels high-latitude oceans where diatoms abundant. Our data provide evolutionary, meta-genomic, functional insights poorly understood yet evolutionarily recurrent pathway.

Language: Английский

Citations

6
Antarctic benthic diatoms after 10 months of dark exposure: consequences for photosynthesis and cellular integrity DOI Creative Commons

Jacob Handy,

Desirée Juchem,

Qian Wang

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: March 22, 2024

Antarctic algae are exposed to prolonged periods of extreme darkness due polar night, and coverage by ice snow can extend such dark conditions up 10 months. A major group microalgae in benthic habitats Antarctica diatoms, which key primary producers these regions. However, the effects extremely exposure on their photosynthesis, cellular ultrastructure, cell integrity remain unknown. Here we show that five strains diatoms exhibit an active photosynthetic apparatus despite months dark-exposure. This was shown a steady effective quantum yield photosystem II (Y[II]) upon light for 2.5 months, suggesting do not rely metabolically inactive resting cells survive darkness. While limnic performed better than marine counterparts, Y(II) recovery values commonly observed occurred after 4-5 all strains, long recovering times. Dark dramatically reduced chloroplast thylakoid stacking, led higher proportion with compromised membranes light-adapted cells. oxygen production readily measurable strong photoinhibition only at high levels (>800 µmol photons m -2 s -1 ). Our data suggest well adapted periods. several followed few another period may prevent them regain full potential times, might compromise long-term population survival.

Language: Английский

Citations

4
Photosynthetic light requirement near the theoretical minimum detected in Arctic microalgae DOI Creative Commons
Clara J. M. Hoppe, Niels Fuchs, Dirk Notz

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 4, 2024

Language: Английский

Citations

4
Evolutionary conservation and metabolic significance of autophagy in algae. DOI
Juliette Laude, Mattéo Scarsini, Charlotte Nef

et al.

PubMed, Journal Year: 2024, Volume and Issue: 379(1914), P. 20230368 - 20230368

Published: Nov. 18, 2024

Autophagy is a highly conserved 'self-digesting' mechanism used in eukaryotes to degrade and recycle cellular components by enclosing them double membrane compartment delivering lytic organelles (lysosomes or vacuoles). Extensive studies plants have revealed how autophagy intricately linked essential aspects of metabolism growth, both normal stress conditions, including organelle homeostasis, nutrient recycling, development, responses biotic abiotic stresses, senescence cell death. However, knowledge regarding autophagic processes other photosynthetic organisms remains limited. In this review, we attempt summarize the current understanding algae from metabolic, molecular evolutionary perspective. We focus on composition conservation machinery discuss role metabolic regulation, homeostasis adaptation algae. This article part theme issue 'The evolution plant metabolism'.

Language: Английский

Citations

3
Photoperiodic dependent regulation of photosynthesis in the polar diatom Fragilariopsis cylindrus DOI Creative Commons
Sébastien Guérin, Flavienne Bruyant, Michel Gosselin

et al.

Frontiers in Photobiology, Journal Year: 2024, Volume and Issue: 2

Published: Aug. 5, 2024

Introduction: Polar microalgae are exposed to dramatic seasonal changes in light availability, from continuous summer days winter nights with rapid of the daylength spring and fall. Under this challenging climate, large diatoms blooms occur at bottom sea-ice underneath icepack, accounting for a significant proportion annual marine primary production Arctic Ocean. The on-going earlier melt down snow ice covers result stronger penetration consequent increase irradiance sea leading diatom under shorter daylengths. Therefore, elucidating response polar different photoperiods will help better understand consequences changing arctic climate on their photosynthetic productivity. Methods: In study, we characterized F. cylindrus , model diatom, across five similar temperature conditions (30 μmol photons m -2 s -1 0°C respectively). Results: We report photoacclimative strategies longer daylengths, special case prolonged darkness (mimicking night). also observed repeated daily regulation photochemistry photoprotection parameters when cells were light:darkness alternation, despite constant optimal intensity during periods. Discussion: Our results highlight ability grow efficiently wide range finely adjusting balance between make best use available light, supporting sustained growth low temperature.

Language: Английский

Citations

2
Microbial response to seasonal variation in Arctic biocrusts with a focus on fungi and cyanobacteria DOI Creative Commons
Ekaterina Pushkareva, Eva Hejduková, Josef Elster

et al.

Environmental Research, Journal Year: 2024, Volume and Issue: 263, P. 120110 - 120110

Published: Oct. 5, 2024

Biocrusts are crucial components of Arctic ecosystems, playing significant roles in carbon and nitrogen cycling, especially regions where plant growth is limited. However, the microbial communities within biocrusts their strategies for surviving harsh conditions remain poorly understood. In this study, profiles across different seasons (summer, autumn, winter) were investigated order to elucidate survival extreme conditions. Metagenomic metatranscriptomic analyses revealed differences community composition among sites located elevations. The bacterial dominated by Actinobacteria Proteobacteria, while fungal mainly represented Ascomycota Basidiomycota, with lichenized saprotrophic traits prevailing. Cyanobacteria primarily composed heterocystous cyanobacteria. Furthermore, study identified molecular mechanisms underlying cold adaptation, including expression heat shock proteins cold-inducible RNA helicases cyanobacteria fungi. Overall, appear be permanently well adapted environment.

Language: Английский

Citations

2
Comparing Diatom Photosynthesis with the Green Lineage DOI
Dany Croteau, Erik L. Jensen, Christian Wilhelm

et al.

Published: July 7, 2024

Diatoms are an important group of eukaryotic phytoplankton, responsible for about a quarter CO 2 fixation on Earth. Oxygenic photosynthesis uses the same building blocks throughout species diversity, with highly conserved photosynthetic complexes and Calvin-Benson-Bassham cycle enzymes. However, many differences observed in spatial organization apparatus regulation photochemical chemical phases photosynthesis. Here, we describe similarities between diatom that green lineage. We focus complexes, different routes electron transfers, concentration fixation, orchestration general response to environmental changes.

Language: Английский

Citations

1
Long days and long nights: an integrative study reveals survival strategies of an Antarctic diatom during the cold and dark polar winter DOI Creative Commons
Rachael M. Morgan‐Kiss

New Phytologist, Journal Year: 2024, Volume and Issue: 241(5), P. 1885 - 1887

Published: Jan. 13, 2024

This article is a Commentary on Joli et al . (2024), 241 : 2193–2208

Language: Английский

Citations

0
Exploring controls on the timing of the phytoplankton bloom in western Baffin Bay, Canadian Arctic DOI Creative Commons
Maxime Benoît‐Gagné, Stephanie Dutkiewicz, Inge Deschepper

et al.

Elementa Science of the Anthropocene, Journal Year: 2024, Volume and Issue: 12(1)

Published: Jan. 1, 2024

In the Arctic Ocean peak of phytoplankton bloom occurs around period sea ice break-up. Climate change is likely to impact phenology and its crucial contribution production dynamics marine ecosystems. Here we explore quantify controls on timing spring using a one-dimensional biogeochemical/ecosystem model configured for coastal western Baffin Bay. The reproduces observations made assemblage community from an camp in region. Using sensitivity experiments, found that two essential were biomass before initiation light under level nitrate was less important. delayed up 20 days if overwintering too low. This result highlights importance survival mechanisms during polar winter pelagic ecosystem dynamics.

Language: Английский

Citations

0
Complementary environmental analysis and functional characterization of a plastid diatom lower glycolytic-gluconeogenesis pathway DOI Creative Commons
Richard G. Dorrell, Youjun Zhang, Yue Liang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Sept. 9, 2022

Abstract Organic carbon fixed in chloroplasts through the Calvin Cycle can be diverted towards different metabolic fates, including cytoplasmic and mitochondrial respiration; gluconeogenesis; synthesis of diverse plastid metabolites via pyruvate hub. In plants, is principally produced glycolysis, although a plastid-targeted lower glycolytic pathway known non-photosynthetic tissue. Here, we characterize glycolytic-gluconeogenesis diatoms, ecologically important marine algae distantly related to plants. We show that two reversible enzymes required complete diatom glycolysis-gluconeogenesis, Enolase PGAM ( bis- phospho-glycerate mutase), originated duplications mitochondria-targeted respiratory isoforms. Through CRISPR-Cas9 mutagenesis, integrative ‘omic analyses, measured kinetics expressed Phaeodactylum tricornutum , present evidence this diverts glyceraldehyde-3-phosphate into hub, may also function gluconeogenic direction. Considering experimental data, has roles dependent particular on day length environmental temperature, it at elevated levels high latitude oceans where diatoms are abundant. Our data provide evolutionary, meta-genomic functional insights poorly understood yet evolutionarily recurrent pathway.

Language: Английский

Citations

2