From Spear to Shield: A Novel Antifungal Drug that Safely Enhances Rice Immune Defenses DOI

Bin Bin Huang,

Ming Wei Gao,

Xi Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

The infection and resistance of pathogenic fungi pose a significant threat to food crop safety, highlighting the urgent need for new green antifungal agents. Fusarium acid (FA) with its simple novel structure correlates principles pesticides has demonstrated substantial broad-spectrum activity. In previous study, several promising lead compounds were identified. This study focused on further optimizing compound A1. Its inhibitory effect was evaluated against six different Notably, anti-Magnaporthe oryzae (M. oryzae) activity B4 significantly enhanced an EC50 value 910 ng/mL. Rice safety tests indicated that not only exhibited no adverse rice growth chlorophyll synthesis but also induced plant immunity by increasing levels polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), soluble sugars. Further investigations into mechanism it permeability damage mycelial membrane, thereby leading electrolyte leakage loss essential cellular substances. Subsequently, production reactive oxygen species (ROS) in M. cell, including •OH, •O2–, 1O2, while promoting increased malondialdehyde (MDA) SOD enzyme effectively inhibited conidia melanin production. These findings FA derivatives hold potential as strategy disease management offer valuable insights control fungal diseases.

Language: Английский

Investigation of the mechanisms involved in the biocontrol activities of natural products from a marine soil bacterium against rice blast DOI Creative Commons
Liwang Fei, Rahila Hafeez, Junliang Zhang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Abstract BACKGROUND Rice blast, caused by Pyricularia oryzae , is a devastating fungal disease threatening global rice production. Overreliance on chemical fungicides has raised environmental concerns and led to resistant strains, necessitating the development of sustainable alternatives. This study integrated marine microbiology natural antifungal compounds create eco‐friendly alternatives for management. RESULTS We identified Pseudomonas aeruginosa R64 with broad‐spectrum antimicrobial activity from mangrove soil in Mai Po Nature Reserve. The fermentation extract (RFE) exhibited multifaceted inhibition P. suppressing mycelial growth, conidiation, conidial germination appressorial formation, while disturbing cell wall membrane function. It also attenuated virulence impairing penetration invasive growth. Further analysis phenazines quinolines as primary RFE, corroborated PCR detection corresponding phenazine biosynthetic gene clusters. Comparative bioassays two main bioactive components phenazine‐1‐carboxamide (PCN) phenazine‐1‐carboxylic acid (PCA), against implicated PCN principal effector. RFE had higher efficacy than tricyclazole growth inhibition, but were less effective isoprothiolane. Furthermore, displayed lower acute ecotoxicity an indicator organism isoprothiolane, suggesting their potential biopesticides blast CONCLUSION Natural products bacterium inhibited key developmental infection processes effectively reducing development. promising low mangrove‐associated bacteria highlight untapped innovative, fungicide mining agriculture. © 2025 Author(s). Pest Management Science published John Wiley & Sons Ltd behalf Society Chemical Industry.

Language: Английский

Citations

0
From Spear to Shield: A Novel Antifungal Drug that Safely Enhances Rice Immune Defenses DOI

Bin Bin Huang,

Ming Wei Gao,

Xi Wang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

The infection and resistance of pathogenic fungi pose a significant threat to food crop safety, highlighting the urgent need for new green antifungal agents. Fusarium acid (FA) with its simple novel structure correlates principles pesticides has demonstrated substantial broad-spectrum activity. In previous study, several promising lead compounds were identified. This study focused on further optimizing compound A1. Its inhibitory effect was evaluated against six different Notably, anti-Magnaporthe oryzae (M. oryzae) activity B4 significantly enhanced an EC50 value 910 ng/mL. Rice safety tests indicated that not only exhibited no adverse rice growth chlorophyll synthesis but also induced plant immunity by increasing levels polyphenol oxidase (PPO), peroxidase (POD), superoxide dismutase (SOD), soluble sugars. Further investigations into mechanism it permeability damage mycelial membrane, thereby leading electrolyte leakage loss essential cellular substances. Subsequently, production reactive oxygen species (ROS) in M. cell, including •OH, •O2–, 1O2, while promoting increased malondialdehyde (MDA) SOD enzyme effectively inhibited conidia melanin production. These findings FA derivatives hold potential as strategy disease management offer valuable insights control fungal diseases.

Language: Английский

Citations

0