Embolism propagation does not rely on pressure only: time-based shifts in xylem vulnerability curves of angiosperms determine the accuracy of the flow-centrifuge method DOI
Luciano M. Silva, Jonas Pfaff, Luciano Pereira

et al.

Tree Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Centrifuges provide a fast approach to quantify embolism resistance of xylem in vulnerability curves (VCs). Since formation is assumingly driven by pressure only, spin time not standardised for flow centrifuge experiments. Here, we explore what extent could be spin-time dependent, and hypothesise that changes hydraulic conductivity (Kh) would shift VCs towards higher water potential (Ψ) values over time. We quantified time-based shifts flow-centrifuge their parameter estimations six angiosperm species measuring Kh 15 minutes spinning at particular speed, before speed was applied the same sample. compared various per sample based on cumulative time, modelled relationship between Kh, Ψ, spin-time. Time-based showed considerable increases decreases low high speeds, respectively, which generally shifted more positive Ψ values. Values corresponding 50% loss (P50) became less negative up 0.72 MPa Acer pseudoplatanus, average 8.5% all did consider By employing an asymptotic exponential model, estimated time-stable improved statistical significance 5 6 studied. This model also revealed instability short times with flow-centrifuges following saturating growth curve. Although remains major determinant formation, should considered because considering time-dependent stability overestimates resistance. artefact species-specific, likely relatively slow gas diffusion associated propagation. The accuracy determining each without considerably extending experimental construct VCs.

Language: Английский

Gas diffusion kinetics in relation to embolism formation and propagation in angiosperm xylem: a mini-review DOI
Lincon Matheus Araujo Silva,

B. Bujnowski,

Luciano Pereira

et al.

Acta Horticulturae, Journal Year: 2025, Volume and Issue: 1419, P. 123 - 134

Published: Feb. 1, 2025

Language: Английский

Citations

0
Channel deformations during elastocapillary spreading of gaseous embolisms in biomimetic leaves DOI
François-Xavier Gauci, Ludovic Jami, Ludovic Keiser

et al.

Interface Focus, Journal Year: 2025, Volume and Issue: 15(2)

Published: May 16, 2025

The nucleation and/or spreading of bubbles in water under tension (due to evaporation) can be problematic for most plants along the ascending sap network-from roots leaves-called xylem. Due global warming, trees facing drought conditions are particularly threatened by formation such embolisms, which hinders flow and ultimately fatal. Polydimethylsiloxane (PDMS)-based biomimetic leaves simulating evapotranspiration have demonstrated that, a linear configuration, existence slender constriction channel allows creation intermittent embolism propagation (as an interaction between elasticity leaf capillary forces at air/water interfaces) (Keiser et al. 2022 J. Fluid Mech. 948, A52 (doi:10.1017/jfm.2022.733); Keiser 2024 R. Soc. Interface 21, 20240103 (doi:10.1098/rsif.2024.0103)). Here, we use analogue PDMS-based one dimension two dimensions. To better explore mechanism, add setup additional technique, allowing measure directly microchannel's ceiling deformation versus time, corresponds pressure variations. We present here method that quantitative insights into dynamics spreading. coupling deformations Laplace threshold explains observed elastocapillary dynamics.

Language: Английский

Citations

0
Embolism propagation in Adiantum leaves and in a biomimetic system with constrictions DOI
Ludovic Keiser, Benjamin Dollet, Philippe Marmottant

et al.

Journal of The Royal Society Interface, Journal Year: 2024, Volume and Issue: 21(217)

Published: Aug. 1, 2024

Drought poses a significant threat to forest survival worldwide by potentially generating air bubbles that obstruct sap transport within plants’ hydraulic systems. However, the detailed mechanism of entry and propagation at scale veins remains elusive. Building upon biomimetic model leaf which we developed, propose direct comparison embolism in Adiantum (maidenhair fern) leaves, presented Brodribb et al . (Brodribb TJ, Bienaimé D, Marmottant P. 2016 Revealing catastrophic failure networks under stress. Proc. Natl Acad. Sci. USA 113 , 4865–4869 (doi: 10.1073/pnas.1522569113 )) our leaves. In particular, evidence jerky dynamics observed leaves can be recovered through introduction micrometric constrictions section veins, mimicking nanopores present bordered pit membranes real We show intermittency retrieved simple coupling variations pressure induced volume compliant microchannels. Our study marks step with design reproduces particular aspects using minimal set controllable readily tunable components. This constitutes promising physical analogue sets stage for future enhancements fully embody unique features embolizing

Language: Английский

Citations

2
Time-based shifts in xylem vulnerability curves of angiosperms based on the flow-centrifuge method DOI Creative Commons
Luciano M. Silva, Jonas Pfaff, Luciano Pereira

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 3, 2024

Abstract Centrifuges provide a fast and standard approach to quantify embolism resistance of xylem in vulnerability curves (VCs). Traditionally, formation centrifuge experiments is assumingly driven by speed, thus pressure, but unaffected spin time. Here, we explore what extent not only pressure also time dependent, hypothesise that time-stable hydraulic conductivity (K h ) values could shift VCs. We quantified time-based shifts flow- VCs their parameter estimations for six angiosperm species measuring K at regular intervals over 15 minutes spinning particular speed before higher was applied the same sample. compared various per sample based on cumulative time, modelled relationship between , water potential (Ψ), Time-based changes showed considerable increases decreases low high speeds, respectively, which generally shifted towards more positive Ψ values. Values corresponding 50% loss ( P 50 increased up 0.72 MPa Acer pseudoplatanus average 8.5% all did consider By employing an asymptotic exponential model, estimated improved statistical significance 5 6 studied. This model revealed instability short times, flow-centrifuges followed saturating growth curve. Although remains major determinant formation, should be considered avoid overestimation resistance. spin-time artefact species- specific, likely relatively slow gas diffusion associated with spreading. It can minimized determining each without considerably extending experimental construct

Language: Английский

Citations

1
An appreciation of apex‐to‐base variation in xylem traits will lead to more precise understanding of xylem phenotypic plasticity DOI Creative Commons
Giai Petit

New Phytologist, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Summary Xylem air embolism is the primary cause of drought‐related tree mortality. Phenotypic plasticity xylem traits key for species acclimation to environmental variability and evolution. It widely believed that plants increase resistance in response drought. However, I argue this hypothesis, based on extensive literature, relies sampling methods overlook predictable anatomical patterns, potentially biasing our understanding adaptation strategies.

Language: Английский

Citations

1
Embolism propagation does not rely on pressure only: time-based shifts in xylem vulnerability curves of angiosperms determine the accuracy of the flow-centrifuge method DOI
Luciano M. Silva, Jonas Pfaff, Luciano Pereira

et al.

Tree Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 10, 2024

Centrifuges provide a fast approach to quantify embolism resistance of xylem in vulnerability curves (VCs). Since formation is assumingly driven by pressure only, spin time not standardised for flow centrifuge experiments. Here, we explore what extent could be spin-time dependent, and hypothesise that changes hydraulic conductivity (Kh) would shift VCs towards higher water potential (Ψ) values over time. We quantified time-based shifts flow-centrifuge their parameter estimations six angiosperm species measuring Kh 15 minutes spinning at particular speed, before speed was applied the same sample. compared various per sample based on cumulative time, modelled relationship between Kh, Ψ, spin-time. Time-based showed considerable increases decreases low high speeds, respectively, which generally shifted more positive Ψ values. Values corresponding 50% loss (P50) became less negative up 0.72 MPa Acer pseudoplatanus, average 8.5% all did consider By employing an asymptotic exponential model, estimated time-stable improved statistical significance 5 6 studied. This model also revealed instability short times with flow-centrifuges following saturating growth curve. Although remains major determinant formation, should considered because considering time-dependent stability overestimates resistance. artefact species-specific, likely relatively slow gas diffusion associated propagation. The accuracy determining each without considerably extending experimental construct VCs.

Language: Английский

Citations

1