The AP2 transcription factor CsAIL6 negatively regulates citric acid accumulation in citrus fruits by interacting with a WD40 protein CsAN11

Horticulture Research, Journal Year: 2025, Volume and Issue: 12(4)

Published: Jan. 6, 2025

Abstract Citric acid accumulation is an essential process in citrus fruits that determines fruit flavor and marketability. The MBW complex transcription factor genes, CsAN11, CsAN1, CsPH4 play key roles regulating citric accumulation. Although how to regulate CsAN1 or was widely investigated, studies on the regulation of CsAN11 are scarce. In this study, we characterized AP2/ERF (APETALA2/ethylene response factor) gene CsAIL6, which lowly expressed high-acid varieties highly low-acid varieties. Overexpressing CsAIL6 obviously decreased content fruits, calli, tomatoes, whereas silencing significantly increased content. Additionally, transcript levels were by CsAIL6; only level overexpressing CsAIL6. Similarly, tomato AN11 (SIAN11) stably markedly lower than wild-type (WT) fruits. Further experiments revealed organic but had no obvious influence level; addition, interacts with rather complex. Taken together, our findings verified negatively regulates through directly interacting WD40 protein provides a new mechanism for

Language: Английский

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Elevated expression of CsCit and CsALMT9-like plays a key role in lowering citrate and increasing malate accumulation in Citrus tamurana × natsudaidai ‘Haruka’ fruit

Horticultural Plant Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

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Comparative Transcriptomic Profiling Reveals Divergent Drought-Response Mechanisms Between Resistant and Susceptible Apple Genotype Roots

Agronomy, Journal Year: 2025, Volume and Issue: 15(3), P. 748 - 748

Published: March 20, 2025

Drought stress caused a significant threat to apple growth and production. Although there is an increasing studies concerning the molecular mechanism in response drought, most of these focus on leaves. However, roots first organism sense drought signal play important role response. The mechanisms underlying needs be further explored. In this study, we conducted comparative transcriptomic analysis from drought-resistant (Malus prunifolia) drought-susceptible hupehensis) genotypes under different soil water contents with 60% (control), 30% (mild drought), 10% (moderate 5% (severe drought). Mapman pathways showed that Malus prunifolia exhibited more rapid activation abscisic acid (ABA) biosynthesis (NCED, PYL) signaling pathway, as well induction transcription factors (NAC, WRKY, MYB) compare mild moderate treatments. This might one reasons why exhibits greater resistance. Furthermore, weighted gene co-expression network (WGCNA) was adopted for identification core drought-responsive genes. Notably, three hub genes, ubiquitin-conjugating enzyme 32 (UBC32), basic leucine-zipper 4 (bZIP4), highly ABA-induced PP2C 2 (HAI2), were selected modules, suggesting their vital roles Taken together, our results gain insights into global expression alterations drought-resistance susceptible germplasms conditions identify some key genes involved response, which helpful breeding future.

Language: Английский

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Analysis of allohexaploid wheatgrass genome reveals its Y haplome origin in Triticeae and high-altitude adaptation

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 1, 2025

Phylogenetic origin of the Y haplome present in allopolyploid Triticeae species remains unknown. Here, we report 10.47 Gb chromosome-scale genome allohexaploid Elymus nutans (StStYYHH). Phylogenomic analyses reveal that is sister to clade comprising V and Jv haplomes from Dasypyrum Thinopyum. In addition, H Hordeum-like ancestor, St Pseudoroegneria-like ancestor are placed successively diverged clades. Resequencing data origins with St, Y, combinations Elymus. Population genomic indicate E. has expanded medium high/low-altitude regions. Phenotype/environmental association identify MAPKKK18 promoter mutations reducing its expression, aiding UV-B adaptation high-altitude populations. These findings enhance understanding evolution aid breeding forage cereal crops through intergeneric hybridization within Triticeae. unclear. authors assembly nutans, all likely during origins, gene responsible for radiation adaptation.

Language: Английский

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Reassessing Drought Tolerance in Citrus Tetraploid Rootstocks: Myth or Reality?

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Polyploidy, particularly tetraploidy, has emerged as a promising tool in citrus rootstock breeding due to its potential enhance drought tolerance. This review examines the role of tetraploid rootstocks resilience, focusing on molecular and physiological adaptations observed controlled environments field conditions. Tetraploids display traits such increased abscisic acid (ABA) production, antioxidant defenses, osmotic adjustments. However, these advantages often fail translate into superior tolerance conditions, where competition for resources environmental complexities significantly influence plant responses. Recent evidence suggests that methodological limitations earlier studies, pots, may have overstated benefits tetraploids. Field studies indicate diploids, with more extensive root systems greater water extraction capacity, outperform tetraploids under stress. To advance breeding, it is essential standardize experimental approaches, control soil matric potential, prioritize long-term studies. Identifying key genes metabolic pathways associated tolerance, along application advanced tools CRISPR/Cas9, will enable development resilient rootstocks, ensuring sustainable production amidst increasing scarcity climate change.

Language: Английский

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CsMYB1‐CwINV6 Module Involves in the Promotion of Soluble Sugar Accumulation in Citrus Fruits Under Drought Stress

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 9, 2025

ABSTRACT Drought can promote soluble sugar accumulation in fruits by increasing the fruit sink strength. Cell wall invertase (CwINV) plays a pivotal role determining strength regulating sucrose partitioning into extracellular matrix. Research has demonstrated that drought stress significantly increases transcript level of citrus CwINV6 , but transcriptional mechanisms governing its regulation under conditions remain elusive. In this study, we characterised MYB transcription factor gene CsMYB1 from genome. is localised cell nucleus, and wall. Furthermore, levels both increased ‘Nanfeng’ tangerine ( Citrus reticulata ) response to or ABA treatment. Transient overexpression promoted glucose fructose fruits. Conversely, transient VIGS resulted opposite trend. Additionally, stable content ‘Micro‐Tom’ tomato lines. Y1H luciferase assays confirmed bind promoter positively regulate expression. Taken together, our findings reveal promotes distribution via ‐CwINV6 module.

Language: Английский

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CsABF8 mediates drought-induced ABA signaling in the regulation of raffinose biosynthesis in Camellia sinensis leaves

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 311, P. 143521 - 143521

Published: April 28, 2025

Language: Английский

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