Regulation of potato late blight resistance genes by different promoters and translation regulators

Horticulture Advances, Journal Year: 2025, Volume and Issue: 3(1)

Published: April 9, 2025

Abstract Late blight, caused by Phytophthora infestans , is one of the most devastative global plant threats. Pyramiding multiple resistance genes ( R genes) was initially considered as a promising strategy for breeding broad-spectrum late blight-resistance potato varieties. However, stacking may induce detrimental effects on health. Fine-tuning expression critical their effective in breeding. This study confirms that low-level individual blight like RB R8 and Vnt1.1 controlled CaMV 35S promoter, does not trigger cell death (CD). co-expression these Nicotiana benthamiana leaves induces CD. Plant upstream open reading frames (uORFs) attenuate translation downstream main ORF. We demonstrates N. frame (NbuORF) Solanum tuberosum L. (StuORF) completely suppress green fluorescent protein (GFP) translation, whereas Arabidopsis thaliana (AtuORFs) only partially inhibit GFP leaves. Simultaneous AtuORFs or StuORF under control either pathogen-inducible vetispiradiene synthase gene 3 (PVS3) promoter leads to inhibition Transgenic potatoes with 35S::RB exhibit superior compared PVS3::RB transgenic lines. Plants expressing 35S::StuORF-RB show increased resistance, although slightly lower than plants. 35S::AtuORFs-RB PVS3::AtuORFs-RB are susceptible P. suggesting These findings highlight potential different uORF combinations finely tuning expression, which optimize balance between normal growth.

Language: Английский

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Sugar transporters: mediators of carbon flow between plants and microbes

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 16

Published: April 16, 2025

Pathogens and symbiotic microorganisms significantly influence plant growth crop productivity. Enhancing disease resistance maximizing the beneficial role of in agriculture constitute critical areas scientific investigation. A fundamental aspect plant-microorganisms interactions revolves around nutritional dynamics, characterized by either "food shortage" or supply" scenarios. Notably, pathogenic predominantly utilize photosynthetic sugars as their primary carbon source during host colonization. This phenomenon has generated substantial interest regulatory mechanisms governing sugar transport redistribution at plant-microorganism interface. Sugar transporters, which primarily mediate allocation to various sink organs, have emerged crucial players plant-pathogen establishment associations. review systematically categorized transporters highlighted functional significance mediating with microorganisms. Furthermore, we synthesized recent advancements understanding molecular these identified key questions warranting further Elucidating roles offers novel strategies for enhancing health productivity, thereby contributing agricultural sustainability global food security.

Language: Английский

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Accumulation of dually targeted StGPT1 in chloroplasts mediated by StRFP1, an E3 ubiquitin ligase, enhances plant immunity

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(11)

Published: Aug. 28, 2024

Abstract Chloroplasts play a crucial role in essential processes, such as photosynthesis and the synthesis of primary diverse secondary metabolites. Recent studies have also highlighted their significance linked to phytohormone production plant immunity, especially SA JA. Ubiquitination, key posttranslational modification, usually leads target protein degradation, which acts signal for remodeling proteome via induction endocytosis or targeting other membrane associated systems. Previously, potato E3 ligase StRFP1 was shown enhance resistance against Phytophthora infestans, but its mechanism remained unclear. Here, we demonstrate that interacted with dually localized plastid glucose 6-phosphate transporter StGPT1 on endoplasmic reticulum (ER). Transiently expressed StGPT1-GFP located chloroplast ER cells. Overexpression enhances late blight Nicotiana benthamiana, activates immune responses, including ROS bursts up-regulation PTI marker genes. The function seems be related dual localization. Remarkably, ubiquitinates at ER, possibly due merely transient peroxisomes, leading apparent accumulation chloroplasts. Our findings point novel by contributes immunity interacting targeted GPT1

Language: Английский

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Potato NPH3/RPT2‐like (NRL) member StNRL‐9 interacts with Stphots and negatively regulates late blight resistance

Physiologia Plantarum, Journal Year: 2024, Volume and Issue: 176(6)

Published: Oct. 30, 2024

Abstract Blue light enhances the susceptibility of Nicotiana benthamiana to Phytophthora infestans , a causative agent late blight disease. Investigating how blue affects potato resistance is an interesting aspect exploring new ways control photoreceptor phototropins (phot1, phot2) and their downstream interact protein StNRL1 have been shown negatively regulate resistance. In order investigate whether other NPH3/RPT2‐Like (NRL) family members are involved in regulating resistance, this study focused on NRL proteins containing RxSxS motif at C‐terminus. Another StNRL‐9, motifs, was found P. N. . Overexpression StNRL‐9 suppresses accumulation reactive oxygen species (ROS) expression PTI marker genes NbWRKY7 NbWRKY8. Similar StNRL1, interacts with receptors Stphot1 Stphot2 cell membrane could promote degradation positive immune regulator StSWAP70. However does not inhibit INF1‐mediated death (ICD), which different from that inhibits ICD, indicating both plant immunity diverse ways. This provides valuable information for further exploration immunity.

Language: Английский

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