Cited by Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology

Applications of CRISPR Technologies in Forestry and Molecular Wood Biotechnology DOI

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11792 - 11792

Published: Nov. 2, 2024

Forests worldwide are under increasing pressure from climate change and emerging diseases, threatening their vital ecological economic roles. Traditional breeding approaches, while valuable, inherently slow limited by the long generation times existing genetic variation of trees. CRISPR technologies offer a transformative solution, enabling precise efficient genome editing to accelerate development climate-resilient productive forests. This review provides comprehensive overview applications in forestry, exploring its potential for enhancing disease resistance, improving abiotic stress tolerance, modifying wood properties, accelerating growth. We discuss mechanisms various systems, including base editing, prime multiplexing strategies. Additionally, we highlight recent advances overcoming key challenges such as reagent delivery plant regeneration, which crucial successful implementation also delve into ethical considerations using gene drive population-level alterations, well importance containment strategies mitigating risks. emphasizes need continued research, technological advancements, extensive long-term field trials, public engagement, responsible innovation fully harness power shaping sustainable future

Language: Английский

Transgene‐free genome editing in poplar DOI

Lennart Hoengenaert, Chantal Anders, Jan Van Doorsselaere

et al.

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Summary Precise gene‐editing methods are valuable tools to enhance genetic traits. Gene editing is commonly achieved via stable integration of a cassette in the plant's genome. However, this technique unfavorable for field applications, especially vegetatively propagated plants, such as many commercial tree species, where cannot be segregated away without breaking constitution elite variety. Here, we describe an efficient method generating gene‐edited Populus tremula × P. alba (poplar) trees incorporating foreign DNA into its Using Agrobacterium tumefaciens , expressed base‐editing construct targeting CCoAOMT1 along with ALS genes positive selection on chlorsulfuron‐containing medium. About 50% regenerated shoots were derived from transient transformation and free T‐DNA. Overall, 7% chlorsulfuron‐resistant T‐DNA free, edited gene nonchimeric. Long‐read whole‐genome sequencing confirmed absence any tested lines. Additionally, evaluated CodA negative marker eliminate lines that stably incorporated their Although latter not essential selecting transgene‐free, shoots, it may prove other genotypes or varieties.

Language: Английский

Citations

Crash‐and‐dash: a new era in tree genome editing DOI