The dorsal part of the anterior tuberal nucleus responds to auditory stimulation in zebrafish (Danio rerio)
eNeuro,
Journal Year:
2024,
Volume and Issue:
11(7), P. ENEURO.0062 - 24.2024
Published: June 25, 2024
The
zebrafish,
a
widely
used
model
in
neurobiology,
relies
on
hearing
aquatic
environments.
Unfortunately,
its
auditory
pathways
have
mainly
been
studied
larvae.
In
this
study,
we
examined
the
involvement
of
anterior
tuberal
nucleus
(AT)
processing
adult
zebrafish.
Our
tract-tracing
experiments
revealed
that
dorsal
subdivision
AT
is
strongly
bidirectionally
connected
to
central
torus
semicircularis
(TSc),
major
fishes.
Immunohistochemical
visualization
ribosomal
protein
S6
(pS6)
phosphorylation
map
neural
activity
response
stimulation
substantiated
finding:
but
not
ventral
part
responded
stimulation.
A
similar
was
present
TSc
isthmi,
visual
region,
which
as
control
for
testing
if
pS6
activation
specific
We
also
measured
time
course
phosphorylation,
previously
unreported
teleost
fish.
After
stimulation,
found
peaked
between
100
and
130
min
returned
baseline
levels
after
190
min.
This
information
will
be
valuable
design
future
experiments.
results
suggest
an
anatomical
functional
AT,
where
only
connects
network
processes
information.
Language: Английский
Neurochemical Characterization of Dopaminoceptive Cells in Song Control Nuclei of Canaries and Their Activation During Song Production: A Multiplex Fluorescent In Situ Hybridization Study
The Journal of Comparative Neurology,
Journal Year:
2024,
Volume and Issue:
532(10)
Published: Oct. 1, 2024
ABSTRACT
Highly
sensitive
in
situ
hybridization
procedures
(RNAScope)
were
used
to
quantify
the
expression
of
three
dopamine
receptors
(Drd1,
Drd2,
and
Drd3)
two
song
control
nuclei
(HVC
Area
X
basal
ganglia)
that
are
known
receive
dopaminergic
inputs
periaqueductal
gray
(PAG)
male
female
canaries.
Both
sexes
treated
with
testosterone
ensure
they
would
sing
actively.
We
also
determined
excitatory
versus
inhibitory
phenotype
cells
expressing
these
as
well
their
activation
following
a
period
production.
The
receptor
types
identified
each
brain
area,
exception
Drd3
X.
density
varied
function
type
area.
Surprisingly
few
sex
differences
detected;
do
not
seem
explain
testosterone‐induced
song.
Overall,
Drd‐positive
was
much
lower
PAG
than
nuclei.
In
HVC,
majority
subtypes
VGlut2‐positive,
whereas
colocalization
Vglut2
occurred
an
intermediate
proportion
PAG.
number
limited.
Most
dopaminoceptive
did
express
either
or
markers.
Finally,
cellular
during
singing
behavior,
measured
by
Egr1,
observed
subtypes,
except
Language: Английский