Chemical Analysis of Plasma-Activated Culture Media by Ion Chromatography

Pharmaceuticals, Journal Year: 2025, Volume and Issue: 18(2), P. 199 - 199

Published: Feb. 1, 2025

Background: Currently, the procedures and methods applied in biological medical fields for determination of reactive oxygen nitrogen species (RONS), primarily rely on spectrophotometric techniques, which involve use colorimetric reagents. While these are widely accepted, they exhibit significant limitations from an analytical standpoint, particularly due to potential inaccuracies, artifacts, pronounced susceptibility matrix effects. The purpose this Technical Note is demonstrate application ion chromatography—a robust well-established technique—for quantification RONS produced cell culture media through exposure cold atmospheric plasma (CAP), innovative therapeutic approach cancer treatment, known as CAP indirect treatment. In addition, present protocol proposes apply pharmacokinetics principles generated plasma-treated liquids (PTLs) following exposure. Methods: strategy involves elucidating kinetic profiles certain characteristic by evaluating their half-life specific used cultures investigating “pharmacokinetic” (PK) profile. drug dose represented power infusion time corresponds medium CAP. Volume-dependent results were shown, focusing nitrites nitrates activities, justifying cellular inhibition. Results: This methodology enables correlation PTL effects different lines with PK (dose/time) obtained via chromatography. Conclusions: conclusion, being a simple green method, it could be alternative toxic reactions techniques higher detection limits, while achieving good resolution.

Language: Английский

---

Antitumor Potential of Different Treatment Approaches Using Cold Atmospheric Pressure Plasma on Oral Squamous Cell Carcinoma Models: In Vitro Study

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 443 - 443

Published: Feb. 11, 2025

Background/Objectives: Cold atmospheric plasma (CAP) has shown a strong anticancer effect on variety of tumors, presenting new approach for the effective treatment oral squamous cell carcinoma (OSCC), one most prevalent malignant neoplasms with high mortality rate. Here, we aimed to comprehensively investigate antitumor potential two approaches CAP both two-dimensional and three-dimensional OSCC line models, as well analyze whether enhances sensitivity chemotherapy. Methods: An in-house designed needle, helium working gas, was used treat SCC-25 directly or indirectly via plasma-treated medium (PTM). The assessed by measuring viability, apoptosis, adhesion, migration. In addition, combined PTM cisplatin analyzed in tumor spheroids, more complex reliable vitro model. Results: Both treatments showed time-dependent effects affecting their rate apoptosis higher after incubation is mediated intrinsic pathway. By utilizing 3D spheroid model, confirmed additionally demonstrated an increased chemosensitivity PTM-treated cells. Conclusions: results our study illustrate promising avenue application therapeutic option OSCC, either standalone combination cisplatin.

Language: Английский

---

Novel benzofuran-conjugated indolin-2-ones as anticancer agents; design, synthesis, biological assessments, and molecular modeling insights

Bioorganic Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108494 - 108494

Published: April 1, 2025

Language: Английский

---

Human head and neck cancer cell lines response to cold atmospheric plasma activated media is affected by the chemistry of culture media

Heliyon, Journal Year: 2024, Volume and Issue: 11(1), P. e41458 - e41458

Published: Dec. 25, 2024

Language: Английский

---