Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9 DOI Creative Commons

Kaoutar El‐Mounadi,

María Luisa Morales-Floriano,

Hernán García-Ruíz

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Feb. 13, 2020

The terms genome engineering, editing and gene editing, refer to modifications insertions, deletions, substitutions) in the of a living organism. most widely used approach nowadays is based on Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9 (CRISPR-Cas9). In prokaryotes, CRISPR-Cas9 an adaptive immune system that naturally protects cells from DNA virus infections. has been modified create versatile technology wide diversity applications medicine, agriculture, basic studies functions. growing number monocot dicot plant species enhance yield, quality nutritional value, introduce or tolerance biotic abiotic stress, domestication, other applications. Although biosafety concerns remain, promising with potential contribute food production for benefit Gene This provisional file, not final typeset article human population. Here we review principles, current advances CRISPR-Cas9-based crop improvement. We also address show humans have exposed Cas9 homologues long before use CRISP-Cas9 editing.

Language: Английский

CRISPR/Cas: A powerful tool for gene function study and crop improvement DOI Creative Commons
Dangquan Zhang, Zhiyong Zhang, Turgay Ünver

et al.

Journal of Advanced Research, Journal Year: 2020, Volume and Issue: 29, P. 207 - 221

Published: Oct. 21, 2020

It is a long-standing goal of scientists and breeders to precisely control gene for studying its function as well improving crop yield, quality, tolerance various environmental stresses. The discovery modification CRISPR/Cas system, nature-occurred editing tool, opens an era precision breeding.In this review, we first introduce the brief history followed mechanism application system on study improvement. Currently, genome has been becoming mature cutting-edge biotechnological tool improvement that already used in many different traits crops, including pathogen resistance, abiotic tolerance, plant development morphology even secondary metabolism fiber development. Finally, point out major issues associating with future research directions.Key Scientific Concepts Review: CRISPR/Cas9 robust powerful targeting individual DNA RNA sequence genome. can be target knockin, knockout replacement monitoring regulating expression at epigenome levels by binding specific sequence. Agrobacterium-mediated method still efficient delivering regents into targeted cells. However, other delivery methods, such virus-mediated method, have developed enhanced potentials CRISPR/Cas9-based PAM requirement offers CRISPR/Cas9-targted genetic loci also limits CRISPR/Cas9. Discovering new Cas proteins modifying current enzymes play important role editing. Developing better methods eliminating off-target effects, finding key/master genes controlling growth two directions

Language: Английский

Citations

251
Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives DOI Open Access
Oliver Xiaoou Dong, Pamela C. Ronald

PLANT PHYSIOLOGY, Journal Year: 2019, Volume and Issue: 180(1), P. 26 - 38

Published: March 13, 2019

A review of the recent progress in plant genetic engineering for disease resistance highlights future challenges and opportunities field.

Language: Английский

Citations

247
Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao DOI Creative Commons
Andrew S. Fister,

Lena Landherr,

Siela N. Maximova

et al.

Frontiers in Plant Science, Journal Year: 2018, Volume and Issue: 9

Published: March 2, 2018

Theobroma cacao, the source of cocoa, suffers significant losses to a variety pathogens resulting in reduced incomes for millions farmers developing countries. Development disease resistant cacao varieties is an essential strategy combat this threat, but limited by sources genetic resistance and slow generation time tropical tree crop. In study, we present first application genome editing technology using Agrobacterium-mediated transient transformation introduce CRISPR/Cas9 components into leaves cotyledon cells. As proof concept, targeted Non-Expressor Pathogenesis-Related 3 (TcNPR3) gene, suppressor defense response. After demonstrating activity designed single-guide RNAs (sgRNA) vitro, used Agrobacterium system leaf tissue, identified presence deletions ~30% TcNPR3 copies treated tissues. The edited tissue exhibited increased infection with pathogen Phytophthora tropicalis elevated expression downstream genes. Analysis off-target mutagenesis sequences similar sgRNA target sites high-throughput sequencing did not reveal mutations above background error rates. These results confirm function NPR3 as repressor immune demonstrate powerful functional genomics tool cacao. Several stably transformed somatic embryos were obtained via transformation, ongoing work will test effectiveness approach at whole plant level.

Language: Английский

Citations

246
Plant genome editing with TALEN and CRISPR DOI Creative Commons
Aimee A. Malzahn,

Levi G. Lowder,

Yiping Qi

et al.

Cell & Bioscience, Journal Year: 2017, Volume and Issue: 7(1)

Published: April 24, 2017

Genome editing promises giant leaps forward in advancing biotechnology, agriculture, and basic research. The process relies on the use of sequence specific nucleases (SSNs) to make DNA double stranded breaks at user defined genomic loci, which are subsequently repaired by two main repair pathways: non-homologous end joining (NHEJ) homology directed (HDR). NHEJ can result frameshift mutations that often create genetic knockouts. These knockout lines useful for functional reverse studies but also have applications agriculture. HDR has a variety as it be used gene replacement, stacking, creating various fusion proteins. In recent years, transcription activator-like effector clustered regularly interspaced palindromic repeats (CRISPR) CRISPR associated protein 9 or from Prevotella Francisella 1 emerged preferred SSNs research purposes. Here, we review their plant research, discuss current limitations, predict future directions genome editing.

Language: Английский

Citations

242
CRISPR Crops: Plant Genome Editing Toward Disease Resistance DOI
Thorsten Langner, Sophien Kamoun, Khaoula Belhaj

et al.

Annual Review of Phytopathology, Journal Year: 2018, Volume and Issue: 56(1), P. 479 - 512

Published: July 5, 2018

Genome editing by sequence-specific nucleases (SSNs) has revolutionized biology enabling targeted modifications of genomes. Although routine plant genome emerged only a few years ago, we are already witnessing the first applications to improve disease resistance. In particular, CRISPR-Cas9 democratized use in plants thanks ease and robustness this method. Here, review recent developments its application enhancing resistance against pathogens. future, bioedited resistant crops will become standard tool breeding.

Language: Английский

Citations

237
CRISPR/Cas9-mediated efficient editing in phytoene desaturase (PDS) demonstrates precise manipulation in banana cv. Rasthali genome DOI
Navneet Kaur, Anshu Alok,

Shivani

et al.

Functional & Integrative Genomics, Journal Year: 2017, Volume and Issue: 18(1), P. 89 - 99

Published: Nov. 29, 2017

Language: Английский

Citations

233
Use of CRISPR systems in plant genome editing: toward new opportunities in agriculture DOI Creative Commons
Agnès Ricroch,

Pauline Clairand,

Wendy Harwood

et al.

Emerging Topics in Life Sciences, Journal Year: 2017, Volume and Issue: 1(2), P. 169 - 182

Published: Nov. 10, 2017

Initially discovered in bacteria and archaea, CRISPR–Cas9 is an adaptive immune system found prokaryotes. In 2012, scientists a way to use it as genome editing tool. 2013, its application plants was successfully achieved. This breakthrough has opened up many new opportunities for researchers, including the opportunity gain better understanding of plant biological systems more quickly. The present study reviews agricultural applications related CRISPR from 52 peer-reviewed articles published since 2014. Based on this literature review, main achieve improved yield performance, biofortification, biotic abiotic stress tolerance, with rice (Oryza sativa) being most studied crop.

Language: Английский

Citations

224
RNA Interference Mechanisms and Applications in Plant Pathology DOI

Cristina Rosa,

Yen‐Wen Kuo,

Tongyan Tian

et al.

Annual Review of Phytopathology, Journal Year: 2018, Volume and Issue: 56(1), P. 581 - 610

Published: July 6, 2018

The origin of RNA interference (RNAi), the cell sentinel system widely shared among eukaryotes that recognizes RNAs and specifically degrades or prevents their translation in cells, is suggested to predate last eukaryote common ancestor ( 138 ). Of particular relevance plant pathology plants, but also some fungi, insects, lower eukaryotes, RNAi a primary effective antiviral defense, recent studies have revealed small (sRNAs) involved play important roles other diseases, including those caused by cellular pathogens. Because this, because can be manipulated interfere with expression endogenous genes an intra- interspecific manner, has been used as tool gene function for protection. Here, we review discovery RNAi, canonical mechanisms, experimental translational applications, new RNA-based technologies importance pathology.

Language: Английский

Citations

214
Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas DOI Creative Commons
Shi‐qi An, Neha Potnis, J. Maxwell Dow

et al.

FEMS Microbiology Reviews, Journal Year: 2019, Volume and Issue: 44(1), P. 1 - 32

Published: Oct. 1, 2019

ABSTRACT Xanthomonas is a well-studied genus of bacterial plant pathogens whose members cause variety diseases in economically important crops worldwide. Genomic and functional studies these phytopathogens have provided significant understanding microbial-host interactions, virulence host adaptation mechanisms including microbial ecology epidemiology. In addition, several strains are as producers the extracellular polysaccharide, xanthan, used food pharmaceutical industries. This polymer has also been implicated phases disease cycle. this review, we summarise current knowledge on infection strategies regulatory networks controlling from species discuss novel opportunities that body work for control health.

Language: Английский

Citations

212
Efficient Targeted Mutagenesis in Apple and First Time Edition of Pear Using the CRISPR-Cas9 System DOI Creative Commons

Aurélie Charrier,

Emilie Vergne,

Nicolas Dousset

et al.

Frontiers in Plant Science, Journal Year: 2019, Volume and Issue: 10

Published: Feb. 6, 2019

Targeted genome engineering has emerged as an alternative to classical plant breeding and transgenic methods improve crop plants. Among other (zinc finger nucleases or TAL effector nucleases) the CRISPR/Cas system proved be most effective, convenient least expensive method. In this study, we optimized conditions of application on apple explored its feasibility pear. As a proof concept, chose knock-out Phytoene Desaturase (PDS) Terminal Flower 1 (TFL1) genes. To edition efficiency, two different single guide RNAs (gRNAs) were associated Cas9 nuclease for each target gene. These gRNAs placed under control U3 U6 promoters. Characteristic albino phenotype was obtained 85 % lines targeted in MdPDS Early flowering observed 93 MdTFL1.1 gene 9 pear PcTFL1.1. Sequencing zones CRIPSR-PDS CRISPR-TFL1.1 showed that induced mutations but at variable frequencies. cases, cut DNA twenty base pairs near protospacer adjacent motif insertions more frequent than deletions substitutions. The profile PDS well TFL1.1 genes chimeric biallelic. Analysis sample potential off-target sequences construct indicated absence cases three mismatches. addition, transient transformation with CRISPR-PDS produced T-DNA free edited lines. Our overall results indicate that, despite occurrence chimerism, is powerful precise method induce mutagenesis first generation

Language: Английский

Citations

204