Plant gene editing through de novo induction of meristems

Nature Biotechnology, Journal Year: 2019, Volume and Issue: 38(1), P. 84 - 89

Published: Dec. 16, 2019

Language: Английский

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CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing

Trends in Plant Science, Journal Year: 2019, Volume and Issue: 24(12), P. 1102 - 1125

Published: Nov. 11, 2019

Language: Английский

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Molecular farming – The slope of enlightenment

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 40, P. 107519 - 107519

Published: Jan. 16, 2020

Language: Английский

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Plant-based biopharmaceutical engineering

Nature Reviews Bioengineering, Journal Year: 2023, Volume and Issue: 1(6), P. 426 - 439

Published: March 21, 2023

Language: Английский

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Plant Molecular Farming – Integration and Exploitation of Side Streams to Achieve Sustainable Biomanufacturing

Frontiers in Plant Science, Journal Year: 2019, Volume and Issue: 9

Published: Jan. 18, 2019

Plants have unique advantages over other systems such as mammalian cells for the production of valuable small molecules and proteins. The benefits cited most often include safety due to absence replicating human pathogens, simplicity because sterility is not required during production, scalability potential open-field cultivation with transgenic plants, speed transient expression potentially providing gram quantities product in less than 4 weeks. Initially there were also significant drawbacks, need clarify feed streams a high particle burden large host cell proteins, but efficient clarification now readily achieved. Several additional emerged reflecting fact that plants are essentially biodegradable, single-use bioreactors. This article will focus on exploitation this concept biopharmaceutical thus improving overall process economics. Specifically, we discuss properties sustainability platform, commercial different biomass side streams. We find incorporating these through rational integration has more double revenue can currently be achieved using plant-based systems.

Language: Английский

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Plant Glycosides and Glycosidases: A Treasure-Trove for Therapeutics

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: April 7, 2020

Plants contain numerous glycoconjugates that are metabolized by specific glucosyltransferases and hydrolysed glycosidases, some also catalyzing synthetic transglycosylation reactions. The documented value of plant-derived to beneficially modulate metabolism is first addressed. Next, focus given the central theme review. therapeutic plant glycosidases discussed as well present production in platforms human used enzyme replacement therapies. increasing knowledge on including structure catalytic mechanism, described. novel insights have allowed design functionalized highly suicide inhibitors glycosidases. These so-called activity-based probes allow unprecedented visualization cross-species. Here special attention paid use such science promote discovery enzymes identification potential chaperones.

Language: Английский

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Applications of multiplex genome editing in higher plants

Current Opinion in Biotechnology, Journal Year: 2019, Volume and Issue: 59, P. 93 - 102

Published: April 10, 2019

Language: Английский

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Highly efficient multiplex editing: one‐shot generation of 8× Nicotiana benthamiana and 12× Arabidopsis mutants

The Plant Journal, Journal Year: 2021, Volume and Issue: 106(1), P. 8 - 22

Published: Feb. 12, 2021

Genome editing by RNA-guided nucleases, such as SpCas9, has been used in numerous different plant species. However, to what extent multiple independent loci can be targeted simultaneously multiplexing not well documented. Here, we developed a toolkit, based on highly intron-optimized zCas9i gene, which allows assembly of nuclease constructs expressing up 32 single guide RNAs (sgRNAs). We this toolkit explore the limits two major model species, and report isolation transgene-free octuple (8×) Nicotiana benthamiana duodecuple (12×) Arabidopsis thaliana mutant lines generation (T1 T2 , respectively). novel counter-selection markers for N. benthamiana, most importantly Sl-FAST2, comparable well-established seed fluorescence marker, FCY-UPP, production toxic 5-fluorouracil presence precursor. Targeting eight genes with an array nine sgRNAs relying FCY-UPP selection non-transgenic T1 identified astonishingly high efficiencies: All analyzed plants carried mutations all (approximately 112/116 target sites edited). Furthermore, 12 24 A. thaliana. Efficiency was significantly lower thaliana, our results indicate Cas9 availability is limiting factor higher-order applications. line combination phenotypic screening amplicon sequencing. The resources presented provide new perspectives how generate complex genotypes or functionally interrogate groups candidate genes.

Language: Английский

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CRISPR-Mediated Engineering across the Central Dogma in Plant Biology for Basic Research and Crop Improvement

Molecular Plant, Journal Year: 2020, Volume and Issue: 14(1), P. 127 - 150

Published: Nov. 3, 2020

The central dogma (CD) of molecular biology is the transfer genetic information from DNA to RNA protein. Major CD processes governing flow include cell cycle, replication, chromosome packaging, epigenetic changes, transcription, posttranscriptional alterations, translation, and posttranslational modifications. are tightly regulated in plants maintain integrity throughout life cycle pass materials next generation. Engineering various involved gene regulation will accelerate crop improvement feed growing world population. CRISPR technology enables programmable editing alter DNA, RNA, or protein, which would have been impossible past. Here, an overview recent advancements tool development CRISPR-based modulations that expedite basic applied plant research provided. Furthermore, applications major thriving areas research, such as discovery (allele mining cryptic activation), introgression (de novo domestication haploid induction), application desired traits beneficial farmers consumers (biotic/abiotic stress-resilient crops, factories, delayed senescence), described. Finally, global regulatory policies, challenges, prospects for CRISPR-mediated discussed.

Language: Английский

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Current state-of-the-art in plant-based antibody production systems

Biotechnology Letters, Journal Year: 2019, Volume and Issue: 41(3), P. 335 - 346

Published: Jan. 25, 2019

Language: Английский

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