Contribution of phenylpropanoid metabolism to plant development and plant–environment interactions

Journal of Integrative Plant Biology, Journal Year: 2020, Volume and Issue: 63(1), P. 180 - 209

Published: Dec. 16, 2020

Abstract Phenylpropanoid metabolism is one of the most important metabolisms in plants, yielding more than 8,000 metabolites contributing to plant development and plant–environment interplay. materialized during evolution early freshwater algae that were initiating terrestrialization land plants have evolved multiple branches this pathway, which give rise including lignin, flavonoids, lignans, phenylpropanoid esters, hydroxycinnamic acid amides, sporopollenin. Recent studies revealed many factors participate regulation metabolism, modulate homeostasis when undergo successive developmental processes are subjected stressful environments. In review, we summarize recent progress on elucidating contribution coordination interaction, metabolic flux redirection among diverse routes. addition, our review focuses at transcriptional, post‐transcriptional, post‐translational, epigenetic levels, response phytohormones biotic abiotic stresses.

Language: Английский

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Response Mechanism of Plants to Drought Stress

Horticulturae, Journal Year: 2021, Volume and Issue: 7(3), P. 50 - 50

Published: March 13, 2021

With the global climate anomalies and destruction of ecological balance, water shortage has become a serious problem facing all mankind, drought key factor restricting development agricultural production. Therefore, it is essential to study tolerance crops. Based on previous studies, we reviewed effects stress plant morphology physiology, including changes external internal structure root, stem, leaf, osmotic regulation substances, drought-induced proteins, active oxygen metabolism plants. In this paper, main signals signal transduction pathways in plants are described, functional genes regulatory related listed, respectively. We summarize above aspects provide valuable background knowledge theoretical basis for future agriculture, forestry breeding, cultivation.

Language: Английский

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The miR156/SPL module regulates apple salt stress tolerance by activating MdWRKY100 expression

Plant Biotechnology Journal, Journal Year: 2020, Volume and Issue: 19(2), P. 311 - 323

Published: Aug. 9, 2020

Salt stress dramatically impedes plant growth and development as well crop yield. The apple production regions are reduced every year, because of the secondary salt damage by improper fertilization irrigation. To expand cultivation area (Malus domestica) select salt-resistant varieties, mechanism tolerance in is necessary to be clarified. miR156/SPL regulatory module plays key roles embryogenesis, morphogenesis, life cycle stage transformation, flower formation other processes. However, its mechanisms unknown. In order elucidate 156/SPL regulating apple, we performed RLM-5' RACE stable genetic transformation technology verify that both mdm-MIR156a MdSPL13 responded latter was target former. MIR156a overexpression weakened resistance whereas strengthened it. A total 6094 differentially expressed genes relative nontransgenic plants were found RNA-Seq analysis MdSPL13OE. Further verification indicated targeted MdWRKY100 gene promoter. Moreover, enhanced apple. Our results revealed regulates up-regulating This study first underlying miRNA network response provides theoretical empirical bases resources for molecular breeding

Language: Английский

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MdMYB305–MdbHLH33–MdMYB10 regulates sugar and anthocyanin balance in red‐fleshed apple fruits

The Plant Journal, Journal Year: 2023, Volume and Issue: 113(5), P. 1062 - 1079

Published: Jan. 6, 2023

SUMMARY Sugar and anthocyanin are important indicators of fruit quality, understanding the mechanism underlying their accumulation is essential for breeding high‐quality fruit. We identified an R2R3‐MYB transcription factor MdMYB305 in red‐fleshed apple progeny, which was positively correlated with sugar content but negatively content. Transient injection, stable expression [overexpressing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated protein 9 (Cas9)], heterologous transformation tomato confirmed that promotes inhibits synthesis anthocyanin. A series molecular experiments (such as electrophoretic mobility shift luciferase assays) combines sugar‐related genes ( MdCWI1 / MdVGT3 MdTMT2 ) anthocyanin‐related MdF3H MdDFR MdUFGT ), promoting inhibiting activities, finally regulating fruits. In addition, study also found competes MdMYB10 MdbHLH33 binding site to balance fruits, provides a reference value exploring more functions MYB‐bHLH‐MYB complex relationship between future.

Language: Английский

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An R2R3‐MYB network modulates stem strength by regulating lignin biosynthesis and secondary cell wall thickening in herbaceous peony

The Plant Journal, Journal Year: 2023, Volume and Issue: 113(6), P. 1237 - 1258

Published: Jan. 12, 2023

Stem strength is an important agronomic trait affecting plant lodging, and plays essential role in the quality yield of plants. Thickened secondary cell walls stems provide mechanical that allows plants to stand upright, but regulatory mechanism wall thickening stem cut flowers remains unclear. In this study, first, a total 11 non-redundant Paeonia lactiflora R2R3-MYBs related were identified isolated from cut-flower herbaceous peony, among which PlMYB43, PlMYB83 PlMYB103 most upregulated differentially expressed genes. Then, expression characteristics revealed these three specifically acted as transcriptional activators. Next, biological function verification showed P. positively regulated strength, thickness lignin deposition. Furthermore, yeast-one-hybrid dual luciferase reporter assays demonstrated they could bind promoter caffeic acid O-methyltransferase gene (PlCOMT2) and/or laccase (PlLAC4), two key genes involved biosynthesis. addition, PlLAC4 increasing deposition was confirmed by virus-induced silencing overexpression. Moreover, also act activator PlMYB43. The findings study propose network modulating biosynthesis for improving lodging resistance, resource molecular genetic engineering breeding flowers.

Language: Английский

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The StbHLH47 transcription factor negatively regulates drought tolerance in potato (Solanum tuberosum L.)

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 4, 2025

Drought stress is a major environmental constraint affecting crop yields. Plants in agricultural and natural environments have developed various mechanisms to cope with drought stress. Identifying genes associated tolerance potato elucidating their regulatory crucial for the breeding of new germplasms. The bHLH transcription factors involved play roles not only plant development growth but also responsesresponse abiotic In this study, StbHLH47 gene, which highly expressed leaves, was cloned isolated. Subcellular localization assays revealed that gene performs transcriptional functions nucleus, as evidenced by increased malondialdehyde (MDA) content relative conductivity under These findings indicate overexpressing plants are more sensitive Differential expression analysis wild-type (WT) (OE-StbHLH47) significantly differentially were enriched metabolic pathways, biosynthesis secondary metabolites, hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway-plant, phenylpropanoid biosynthesis, plant‒pathogen interactions. Among these phenylalanine abscisic acid (ABA) transduction pathways greater number genes, trends (DEGs) different between WT OE-StbHLH47. Therefore, it speculated may regulate resistance mainly through two pathways. Additionally, RT‒qPCR used fluorescence quantification StNCED1 StERD11, known resistance, results levels much lower OE-StbHLH47 than plants. RNA-seq, RT‒qPCR, physiological index analyses conditions overexpression sensitivity stress, indicating negatively regulates summary, our negative regulator provide theoretical basis further studies on molecular mechanism involved.

Language: Английский

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Combined Transcriptomic and Metabolomic Analysis Reveals the Role of Phenylpropanoid Biosynthesis Pathway in the Salt Tolerance Process of Sophora alopecuroides

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(5), P. 2399 - 2399

Published: Feb. 27, 2021

Salt stress is the main abiotic that limits crop yield and agricultural development. Therefore, it imperative to study effects of salt on plants mechanisms through which respond stress. In this study, we used transcriptomics metabolomics explore Sophora alopecuroides. We found incurred significant gene expression metabolite changes at 0, 4, 24, 48, 72 h. The integrated transcriptomic metabolomic analysis revealed differentially expressed genes (DEGs) differential metabolites (DMs) obtained in phenylpropanoid biosynthesis pathway were significantly correlated under Of these, 28 DEGs seven DMs involved lignin synthesis 23 flavonoid synthesis. Under stress, related changed significantly. Lignin flavonoids may participate removal reactive oxygen species (ROS) root tissue S. alopecuroides reduced damage caused Our research provides new ideas genetic resources mechanism plant responses further improve tolerance plants.

Language: Английский

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How salt stress-responsive proteins regulate plant adaptation to saline conditions

Plant Molecular Biology, Journal Year: 2021, Volume and Issue: 108(3), P. 175 - 224

Published: Dec. 29, 2021

Language: Английский

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Juggling with reactive oxygen species and antioxidant defense system – A coping mechanism under salt stress

Plant Stress, Journal Year: 2022, Volume and Issue: 5, P. 100093 - 100093

Published: May 21, 2022

Even under optimal conditions many metabolic processes produce ROS like superoxide anion (O2.-), hydrogen peroxide (H2O2) and hydroxyl radicals (OH⋅), particularly in chloroplast mitochondria. The overproduction of (O2.-, H2O2, OH., RCO etc.) results from the exposure to various environmental dehydration, heat, salinity biotic stresses. All biomolecules lipids, proteins DNA are extensively damaged by reactive oxygen species which disrupts cell integrity further leading its death. Plants possess both enzymic non-enzymatic mechanism for scavenging ROS. mechanisms designed minimize concentration O2 H2O2. overproduced enzymes dismutase (SOD), peroxidase (POX), catalase (CAT), glutathione reductase (GR) glutathione-synthesizing enzymes. Several evidences have shown that although oxidative stress is a lethal situation (especially H2O2 O2⋅-, it may be involved cellular signaling procedure as second messenger induce large number genes osmoprotectant salt defenses. This review gives an insight into recent advancements on how antioxidant defense machinery, non-antioxidant metabolites work together alleviate negative effects cross-talk with sulfur nitrogen carbomyl also act important signal molecule. comprehensive knowledge about action, their regulation through interactions RNS, RSS RCS transduction will empower us development tolerant plants.

Language: Английский

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Transcription factor ZmWRKY20 interacts with ZmWRKY115 to repress expression of ZmbZIP111 for salt tolerance in maize

The Plant Journal, Journal Year: 2022, Volume and Issue: 111(6), P. 1660 - 1675

Published: July 21, 2022

Maize (Zea mays) is an important cereal crop worldwide. However, its yield and quality are adversely affected by salt stress resulting from soil hypersalinity. Exploring the regulatory mechanisms of responses vital importance to increase maize seed production. In present study, we screened ethyl methanesulfonate-induced mutants identified a salt-tolerant mutant. A single base was mutated in ZmWRKY20, leading formation truncated protein variant. detailed phenotypic analysis revealed that this mutant had significantly higher resistance wilting lower reactive oxygen species levels than inbred line B73. ZmWRKY20 showed transcriptional activity yeast specifically bound W-boxes according results our one-hybrid, electrophoretic mobility shift, dual-luciferase assays. Overexpression decreased tolerance maize. Transcriptome profiling overexpression extensively reprogrammed genes involved regulating defense oxidation-reduction responses. The substantiate directly targeted basic leucine zipper (bZIP) motif transcription factor ZmbZIP111. It also verified interacts with ZmWRKY115 both proteins act jointly enhance ZmbZIP111 repression. indicate factors interact nucleus, repression expression binding promoter, sensitivity seedlings stress. current study improves understanding complicated

Language: Английский

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