Flavones enrich rhizosphere Pseudomonas to enhance nitrogen utilization and secondary root growth in Populus

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 7, 2025

Plant growth behavior is a function of genetic network architecture. The importance root microbiome variation driving plant functional traits increasingly recognized, but the mechanisms governing this are less studied. Here, we collect roots and rhizosphere soils from nine Populus species belonging to four sections (Leuce, Aigeiros, Tacamahaca, Turanga), generate metabolite transcription data for microbiota rhizospheres, conduct comprehensive multi-omics analyses. We demonstrate that vigorous Leuce poplar enrich more Pseudomonas, compared with poorly performing poplar. Moreover, confirm Pseudomonas strongly associated tricin apigenin biosynthesis identify gene GLABRA3 (GL3) critical secretion. elevated secretion via constitutive PopGL3 Chalcone synthase (PopCHS4) can drive colonization in further enhance growth, nitrogen acquisition, secondary development nitrogen-poor soil. This study reveals plant-metabolite-microbe regulation patterns contribute fitness thoroughly decodes key regulatory tricin, provides insights into interactions plant's metabolites its transcriptome microbes.

Language: Английский

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Enhancing active ingredient biosynthesis in Chinese herbal medicine: biotechnological strategies and molecular mechanisms

PeerJ, Journal Year: 2025, Volume and Issue: 13, P. e18914 - e18914

Published: Feb. 10, 2025

Background Chinese herbal medicine (CHM) is a fundamental component of traditional medical practice, offering rich source natural remedies with significant therapeutic potential. However, the scarcity active ingredients and complex extraction procedures present substantial challenges to their widespread clinical application. This review aims address this gap by exploring potential modern biotechnological advancements in enhancing biosynthesis these valuable compounds. Methodology The study takes comprehensive approach, delving into chemical composition CHM’s elucidating biosynthetic pathways molecular regulatory mechanisms. Additionally, it surveys recent progress methodologies evaluates engineering strategies aimed at synthetic production. multifaceted analysis forms foundation for examining role biology augmenting ingredient synthesis. Results Our examination provides insights intricate governing formation ingredients, as well networks that underlie processes. Furthermore, highlights techniques, demonstrating ability streamline enhance isolation Engineering approaches production, including metabolic tools, are assessed overcome limitations scale up Conclusions By integrating from biosynthesis, regulation, methodologies, biology, establishes robust theoretical framework production ingredients. proposed practical guidance aim facilitate broader utilization while promoting sustainability accessibility within invaluable medicinal heritage.

Language: Английский

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Sculpturing Hypericum perforatum L.-rhizosphere with plant-specific and un-specific beneficial rhizobacteria distinctively tailors rhizosphere bacterial community structure to accumulate differential amounts of specialized metabolites

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120670 - 120670

Published: Feb. 13, 2025

Language: Английский

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Dehydration-responsive element-binding transcription factor, GuDREB35, positively regulates drought tolerance in licorice (Glycyrrhiza uralensis)

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 306, P. 141829 - 141829

Published: March 6, 2025

Language: Английский

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Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 29, 2025

Isoflavones such as daidzein and genistein are naturally occurring compounds found in plants legumes. They have diverse pharmacological activities, making them valuable the food, pharmaceutical, cosmetic industries. Currently, isoflavones mainly obtained through extraction of plant biomass. Chemical synthesis is challenging for most due to complexity their structures. The limited supply cannot meet market demands. Advances synthetic biology provided a sustainable efficient solution production isoflavones, with yeasts often serving microbial chassis biosynthesis. This review summarizes properties specific biosynthetic pathways, technical strategies used engineered isoflavone production. In addition, development state-of-the-art biotechnological environmentally friendly bioactive discussed.

Language: Английский

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Research Progress of Genomics Applications in Secondary Metabolites of Medicinal Plants: A Case Study in Safflower

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3867 - 3867

Published: April 19, 2025

Medicinal plants, recognized as significant natural resources, have gained prominence in response to the increasing global demand for herbal medicines, necessitating large-scale production of these plants and their derivatives. are exposed a variety internal external factors that interact influence biosynthesis accumulation secondary metabolites. With rapid development omics technologies such genomics, transcriptomics, proteomics, metabolomics, multi-omics become important tools revealing complexity functionality organisms. They conducive further uncovering biological activities metabolites medicinal clarifying molecular mechanisms underlying Also, artificial intelligence (AI) technology accelerates comprehensive utilization high-dimensional datasets offers transformative potential analysis. However, there is currently no systematic review summarizing genomic metabolite plants. Safflower (Carthamus tinctorius L.) has rich diverse bioactive flavonoids, among which Hydroxysafflor yellow A (HSYA) specific safflower emerging medication treating wide range diseases. Hence, progress been made study an excellent example regulation recent years. Here, we on understanding main at level, summarize various types contents, with particular focus flavonoids. This aims provide insight into regulatory from perspective genomics.

Language: Английский

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Comparing genomes of Fructus Amomi-producing species reveals genetic basis of volatile terpenoid divergence

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: 193(2), P. 1244 - 1262

Published: July 10, 2023

Wurfbainia longiligularis and villosa are both rich in volatile terpenoids 2 primary plant sources of Fructus Amomi used for curing gastrointestinal diseases. Metabolomic profiling has demonstrated that bornyl diphosphate (BPP)-related more abundant the W. seeds have a wider tissue distribution longiligularis. To explore genetic mechanisms underlying terpenoid divergence, high-quality chromosome-level genome (2.29 Gb, contig N50 80.39 Mb) was assembled. Functional characterization 17 terpene synthases (WlTPSs) revealed WlBPPS, along with WlTPS 24/26/28 synthase (BPPS) activity, contributes to BPP-related compared villosa. Furthermore, transgenic Nicotiana tabacum showed GCN4-motif element positively regulates seed expression WvBPPS thus promotes enrichment seeds. Systematic identification analysis candidate TPS 29 monocot plants from 16 families indicated substantial expansion TPS-a TPS-b subfamily genes Zingiberaceae may driven increased diversity production terpenoids. Evolutionary functional BPPS be distributed only plants. This research provides valuable genomic resources breeding improving medicinal edible value sheds light on evolution biosynthesis Zingiberaceae.

Language: Английский

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Metabolites of blueberry roots at different developmental stages strongly shape microbial community structure and intra-kingdom interactions at the root-soil interface

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 947, P. 174333 - 174333

Published: June 28, 2024

Language: Английский

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Effect of supplementation with Glycyrrhiza uralensis extract and Lactobacillus acidophilus on growth performance and intestinal health in broiler chickens

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: July 18, 2024

Intestinal microbiota community is an important factor affecting the nutritional and health status of poultry, its balance crucial for improving overall poultry. The study aimed to investigate effect dietary supplementation with Glycyrrhiza uralensis extract (GUE), Lactobacillus acidophilus (Lac) their combination (GL) on growth performance intestinal in broilers 84-day feeding experiment. Supplementary 0.1% GUE 4.5×10 7 CFU/g Lac significantly increased average daily gain (ADG), GL (0.1% Lac) ADG feed intake (ADFI), decreased conversion rate (FCR) aged 29 84 d 1 d. Dietary GUE, superoxide dismutase (SOD) glutathione peroxidase (GSH-PX) activity Malondialdehyde (MDA) content jejunum mucosa broilers, secretory IgA (sIgA) at Moreover, cecal microbial richness diversity, modulated composition. Both reduced harmful bacteria Epsilonbacteraeota , Helicobacter H. pullorum 28 Proteobacteria Escherichia E. coli d, while beneficial L. gallinarum Compared individual supplementation, markedly SOD sIgA content, . In conclusion, as additives benefit health, combined use shows even more positive broilers.

Language: Английский

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Integration of CRISPR/Cas9 with multi-omics technologies to engineer secondary metabolite productions in medicinal plant: Challenges and Prospects

Functional & Integrative Genomics, Journal Year: 2024, Volume and Issue: 24(6)

Published: Nov. 4, 2024

Language: Английский

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