TaNF-Y-PRC2 orchestrates temporal control of starch and protein synthesis in wheat DOI Open Access
Jinchao Chen, Long Zhao, Haoran Li

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 26, 2023

Abstract The endosperm in cereal grains is instrumental determining grain yield and seed quality, as it controls the production of starch protein. In this study, we identified a specific TaNF-Y trimeric complex, consisting TaNF-YA3-D, TaNF-YB7-B, TaNF-YC6-B, exhibiting robust expression within during filling stage wheat. Knock-down either TaNF-YA3 or TaNF-YC6 led to less but more gluten proteins. Detailed analyses have unveiled that indirectly boosts biosynthesis genes by reducing TaNAC019, repressor TaAGPS1a, TaSuS2 , thereby regulating biosynthesis. Conversely, directly inhibits gliadin low molecular weight (LMW)-GS coding genes, including TaGli-γ-700 TaLMW-400 . Furthermore, components interact with TaSWN, histone methyltransferase subunit Polycomb repressive complex 2 (PRC2), repress TaNAC019 through H3K27me3 modification. Notably, weak mutation TaFIE core PRC2, has reduced elevated LMW-GS levels. Intriguingly, DNA variations are widely associated developmental traits. particular, variation region TaNF-YB7-B linked differences protein content. Distinct haplotypes affect its interaction influencing repression targets like Our findings illuminate intricate mechanisms governing epigenetic regulation TaNF-Y-PRC2 for wheat development. Manipulating holds potential optimizing enhancing quality.

Language: Английский

Genome‑wide identification and expression analysis of the UBC gene family in wheat (Triticum aestivum L.) DOI Creative Commons
Weidong Gao, Long Zhang, Yanyan Zhang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 26, 2024

Ubiquitination is an important regulatory step of selective protein degradation in the plant UPS (ubiquitin-proteasome system), which involved various biological processes eukaryotes. Ubiquitin-conjugating enzymes play intermediate role process ubiquitination reactions and thus essential regulating growth response to adverse environmental conditions. However, a genome-wide analysis UBC gene family wheat (Triticum aestivum L.) has not yet been performed.

Language: Английский

Citations

5
Physicochemical properties of wheat granular flour and quality characteristics of the corresponding fresh noodles as affected by particle size DOI Creative Commons
Jianli Wang,

Tingjing Zhang,

Erqi Guan

et al.

LWT, Journal Year: 2024, Volume and Issue: 204, P. 116439 - 116439

Published: July 1, 2024

Wheat granular flour (GF) is highly nutritious due to low machining precision but the large particle size leads poor processability. By reducing from 224 μm 62 without sifting in present study, chemical compositions, dough rheology, and fresh noodle qualities were tracked, underlying mechanism was preliminarily explored through changes corresponding dough's gluten quality, water–solid interaction, microstructures. Results indicated that only moisture significantly decreased damaged starch signally increased with reduction size. Compared refined flour, all GF samples had higher ash, dietary fiber, beneficial lipids, intact endosperm cells. Noticeably, 91 68 developed quality quantity, stronger water-solids interplay, optimal networks; therefore, resultant doughs displayed a super balance between viscosity elasticity. Accordingly, noodles possessed excellent consumer acceptability water absorption, lower cooking loss, smoothness chewiness after optimum cooking. These observations provide theoretical basis for wheat processing development of healthier wheat-based using as raw material.

Language: Английский

Citations

4
The Molecular Mechanism of Transcription Factor Regulation of Grain Size in Rice DOI

Yanxiu Du,

Chun Ye,

Han Pei-jie

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112434 - 112434

Published: Feb. 1, 2025

Language: Английский

Citations

0
TaFT‐D1 positively regulates grain weight by acting as a coactivator of TaFDL2 in wheat DOI Creative Commons

Yinhui Zhang,

Haixia Liu, Yaojia Wang

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

Summary FLOWERING LOCUS T ( FT ), a multifunctional regulator in crops, modulates multiple key agronomic traits such as flowering time or heading date and plant height; however, its role grain development regulation is unclear. Herein, through genome‐wide association studies (GWAS), we identified TaFT‐D1 , which encodes phosphatidylethanolamine‐binding protein (PEBP), candidate gene for weight wheat. A one‐bp insertion/deletion (InDel) (G/‐) the third exon of resulting different lengths, was significantly associated with weight. knockout via CRISPR‐Cas9 system reduced size weight, increased by promoting cell proliferation starch synthesis. Transcriptome analysis revealed significant decrease expression cycle‐ synthesis‐related genes, including TaNAC019‐3A TaSWEET15‐like‐7B TaCYCD4;1 TaCYCD3;2 taft‐d1 line. interacted bZIP transcription factor TaFDL2, tafdl2 mutant presented relatively small grains, suggesting that TaFDL2 positive size. Moreover, bound to promoters downstream activating their expression, whereas this activation TaFDL2. Interaction assays demonstrated TaFT‐D1, Ta14‐3‐3A formed regulatory complex. Furthermore, TaFT‐D1(G) allele correlated greater thousand‐grain earlier heading. This favourable has undergone strong selection during wheat breeding China. Our findings provide novel insights into how regulates highlight potential application yield improvement

Language: Английский

Citations

0
Insect Resistance in Quercus robur: A Comparative Metabolomic and Transcriptomic Analysis of Normal and Curly Leaf Varieties DOI

K. Liao,

Ruiquan Wang,

Hao Li

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Quercus robur is a tree species that produces acorns with rich nutritional value. Its leaf rolling crucial for adapting to stress, but this trait's impact on plant-insect interactions remains unknown. We compared the resistance phenotypes, metabolomic profiles, and transcriptomic data of curly (Qr-T) normal-leaf (Qr-S) varieties Q. robur. Findings revealed Qr-T exhibited higher area consumption under herbivory. Metabolomic analysis found lower levels key defensive compounds like Questiomycin A, Caffeine, Indoleacrylic acid in Qr-T. Transcriptomics up-regulation DEGs related development (e.g., MYO17, LEC2) down-regulation defense-related (like IOS1, Y3471) GO KEGG analyses indicated defense responses isoflavonoid biosynthesis pathway were suppressed Coexpression network identified coordinated genes, such as CYP81Q32 CYP94A5, their coexpressed transcription factors (such WRKY6, WRKY53) The above findings suggest curling may weaken its insect resistance, which provides clues improving plant agroforestry.

Language: Английский

Citations

0
Epigenetic perspectives on wheat speciation, adaptation, and development DOI
Xuemei Liu, Dongzhi Wang,

Zhaoheng Zhang

et al.

Trends in Genetics, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Cytokinin Translocation to, and Biosynthesis and Metabolism within, Cereal and Legume Seeds: Looking Back to Inform the Future DOI Creative Commons
Paula E. Jameson

Metabolites, Journal Year: 2023, Volume and Issue: 13(10), P. 1076 - 1076

Published: Oct. 13, 2023

Early in the history of cytokinins, it was clear that Zea mays seeds contained not just trans-zeatin, but its nucleosides and nucleotides. Subsequently, both pods legumes cereal grains have been shown to contain a complex cytokinin forms. Relative very high quantities detected developing seeds, only limited amount appears translocated from parent plant. Translocation experiments, detection levels endogenous maternal seed coat tissues legumes, indicates does readily cross maternal/filial boundary, indicating filial are autonomous for biosynthesis. Within seed, trans-zeatin plays key role sink establishment may also contribute strength. The roles, if any, other biologically active forms (cis-zeatin, dihydrozeatin isopentenyladenine) remain be elucidated. recent identification genes coding enzyme leads biosynthesis rice (OsCYP735A3 4), gene an (CPN1) converts riboside apoplast, further cements played by plants.

Language: Английский

Citations

7
Spatial transcriptomics uncover coordinated cellular responses to heat stress in developing wheat grains DOI Creative Commons
Jirui Wang, Yuxin Fu, Xiaojiang Guo

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: May 9, 2024

Abstract The grain-filling stage is highly sensitive to heat, impacting wheat yield and quality. This study uncovered Chuanmai104's grain spatial transcriptome atlas under heat stress, identifying over 120 specifically expressed genes crucial for cell sorting subsequent sequencing across twelve types. differentiation trajectory of endosperm indicated high expression development programmed death at early stages, the enable grains accumulate nutrients. At 15 DAP, recovery stress showed significant gene changes, especially in nucellar projection, near scutellum, embryo. Heat reduced starch ABA synthesis expression, shortening time, reducing weight by 28.9%, raising germination rates from 7.9% 52.0%. Spatial transcriptomics revealed unexpected shifts major domains 189 due featuring three shifts: AL-SCU, ENC-PR, EXC-NP.

Language: Английский

Citations

2
TaDL interacts with TaB3 and TaNF‐YB1 to synergistically regulate the starch synthesis and grain quality in bread wheat DOI Creative Commons

Guoyu Liu,

Runqi Zhang, Zhenlong Wu

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 23, 2024

ABSTRACT Starch biosynthesis is a critical factor in wheat ( Triticum aestivum L.) quality and yield. However, the full scope of its regulation not fully understood. Here we report that TaDL interacts with TaB3 TaNF‐YB1 to synergistically regulate starch wheat. Genome‐edited tadl mutant lines had smaller lighter grains lower total amylose contents compared wild type (WT). Correspondingly, transcript levels biosynthesis‐related genes, including TaSUS1 , TaSUS2 TaAGPL2 TaSBEIIa TaGBSSII TaSWEET2a were markedly at 15 d after flowering (DAF) mutants. physically interacted activated transcription through direct binding their promoter regions. A null also affected grain filling, phenotypes similar those mutants, whereas overexpression promoted filling. Our study demonstrated plays an essential role identified elite allele TaDL‐BI ) associated content, providing insights into underlying molecular mechanism which may be useful breeding high‐yielding improvement.

Language: Английский

Citations

1
Cloning and functional analysis of ZmMADS42 gene in maize DOI Creative Commons
Yang Zhao,

Jianyu Lu,

Bo Hua Hu

et al.

GM crops & food, Journal Year: 2024, Volume and Issue: 15(1), P. 105 - 117

Published: March 11, 2024

Maize (Zea mays L.) is the most important cereal crop in world. Flowering period and photoperiod play roles reproductive development of maize. This study, investigated ZmMADS42, a gene that highly expressed shoot apical meristem. Agrobacterium infection was used to successfully obtain overexpressed ZmMADS42 plants. Fluorescence quantitative PCR revealed expression meristem transgenic plants 2.8 times higher than wild-type(WT). In addition, endosperm 2.4 wild-type. The seed width T2 generation increased by 5.35%, whereas length decreased 7.78% compared with Dissection tips wild-type from 7-leaf stage 9-leaf entered differentiation earlier exhibited more tassel meristems during their vegetative growth period. mature were approximately 20 cm shorter height had lower panicle position Comparing flowering period, tasseling, powdering, silking stages occurred 10 days those results showed played significant role regulating plant

Language: Английский

Citations

0