Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes DOI Creative Commons
Songyuan Zhang,

Songyuan Liu,

Hongpeng Lai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 13, 2024

Abstract In the ongoing plant-pathogen arms race, plants employ pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs), while in successful pathogens, PAMPs can evolve evade detection. Engineering PRRs evading could potentially generate broad-spectrum and durable disease resistance. this study, we reverse-engineered two natural FLAGELLIN SENSING 2 (FLS2) variants, VrFLS2XL GmFLS2b, with extended specificities towards flg22 variants. We identified minimal gain-of-function residues enabling blind FLS2s otherwise uncovered strategies: (i) enhancing FLS2-flg22 interaction around flg22’s key evasion sites, (ii) strengthening direct between FLS2 its co-receptor BAK1 overcome weak agonistic antagonistic flg22s, respectively. Additionally, leveraged polymorphisms that enhance through unknown mechanisms engineer superior capability. These findings offer basic design principles for broader spectra, paving way PRR engineering using precise gene-editing increase resistance crops.

Language: Английский

Advances in the molecular mechanism of grapevine resistance to fungal diseases DOI Creative Commons
Zhi Li, Ronghui Wu, Feng Guo

et al.

Molecular Horticulture, Journal Year: 2025, Volume and Issue: 5(1)

Published: Jan. 2, 2025

Abstract Grapevine is an important economic fruit tree worldwide, but grape production has been plagued by a vast number of fungal diseases, which affect vigor and the quality yield berries. To seek remedies for such issues, researchers have always committed to conventional biotechnological breeding. In recent years, increasing progress made in elucidating molecular mechanisms grape–pathogenic fungi interactions resistance regulation. Here, we summarize current knowledge on basis grapevine including effector-mediated susceptibility resistance, resistant regulatory networks grapevine, innovative approaches genetic transformation, strategies improve resistance. Understanding exploring accurately regulating diseases.

Language: Английский

Citations

2
CRISPR/Cas in Grapevine Genome Editing: The Best Is Yet to Come DOI Creative Commons
Chong Ren,

Mohamed Salaheldin Mokhtar Mohamed,

Nuremanguli Aini

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(9), P. 965 - 965

Published: Sept. 11, 2024

The advent of Clustered Regularly Interspaced Palindromic Repeat (CRISPR)/CRISPR-associated (Cas) proteins as a revolutionary innovation in genome editing has greatly promoted targeted modification and trait improvement most plant species. For grapevine (Vitis vinifera L.), perennial woody species, CRISPR/Cas is an extremely promising technique for genetic short period. Advances have been achieved by using CRISPR technology recent years, which promises to accelerate grapevine. In this review, we describe the development advances CRISPR/Cas9 its orthologs variants. We summarize applications discuss challenges facing well possible strategies that could be used improve addition, outline future perspectives model system, precise editing, accelerated improvement, transgene-free editing. believe will play more important role exciting bright expected economically significant

Language: Английский

Citations

2
A New Approach for CRISPR/Cas9 Editing and Selection of Pathogen-Resistant Plant Cells of Wine Grape cv. ‘Merlot’ DOI Open Access
A. Yu. Fizikova, Zhanneta Zaurovna Tukhuzheva,

Lada Zhokhova

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10011 - 10011

Published: Sept. 17, 2024

Grape is one of the most economically significant berry crops. Owing to biological characteristics grapes, such as long juvenile period (5-8 years), high degree genome heterozygosity, and frequent occurrence inbreeding depression, homozygosity during crossbreeding leads loss varietal viability. CRISPR/Cas editing has become tool choice for improving elite technical grape varieties. This study provides first evidence a decrease in total fraction phenolic compounds an increase concentration peroxide callus cells upon addition chitosan culture medium. These previously unreported metabolic features response have been described used time probability selecting plant with

Language: Английский

Citations

2
Reverse engineering of the pattern recognition receptor FLS2 reveals key design principles of broader recognition spectra against evading flg22 epitopes DOI Creative Commons
Songyuan Zhang,

Songyuan Liu,

Hongpeng Lai

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 13, 2024

Abstract In the ongoing plant-pathogen arms race, plants employ pattern recognition receptors (PRRs) to recognize pathogen-associated molecular patterns (PAMPs), while in successful pathogens, PAMPs can evolve evade detection. Engineering PRRs evading could potentially generate broad-spectrum and durable disease resistance. this study, we reverse-engineered two natural FLAGELLIN SENSING 2 (FLS2) variants, VrFLS2XL GmFLS2b, with extended specificities towards flg22 variants. We identified minimal gain-of-function residues enabling blind FLS2s otherwise uncovered strategies: (i) enhancing FLS2-flg22 interaction around flg22’s key evasion sites, (ii) strengthening direct between FLS2 its co-receptor BAK1 overcome weak agonistic antagonistic flg22s, respectively. Additionally, leveraged polymorphisms that enhance through unknown mechanisms engineer superior capability. These findings offer basic design principles for broader spectra, paving way PRR engineering using precise gene-editing increase resistance crops.

Language: Английский

Citations

0