Advanced Agrochem, Journal Year: 2024, Volume and Issue: 4(1), P. 78 - 89
Published: Nov. 9, 2024
Language: Английский
Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15
Published: Sept. 23, 2024
An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.
Language: Английский
Citations
18
Horticulture Research, Journal Year: 2025, Volume and Issue: 12(4)
Published: Jan. 15, 2025
Abstract Tomato spotted wilt virus (TSWV) poses a significant threat as devastating pathogen to the global production and quality of tomato (Solanum lycopersicum). Mining novel resistance genes within germplasm is an effective environmentally friendly approach combat TSWV. In this study, we investigated mechanisms underlying high TSWV in specific line after experimental inoculation, despite not possessing any known genes. The candidate causal disease traits were finely mapped by constructing different genetic populations performing bulk segregant analysis sequencing. This identified SlDnaJ (Solyc10g081220) key locus potentially regulating resistance. We determined structural variant (designated Sldnaj) containing 61-bp promoter sequence deletion that was specifically present susceptible M82 plant lines. Sldnaj-knockout transgenic plants significantly more resistant than wild-type plants. Up-regulated expression Sldnaj affected salicylic acid/jasmonic acid signaling pathway, which induced promoted systemic infection summary, study new susceptibility gene with natural variation tomato. These findings provide insights into molecular mechanism while offering target for breeding strategies
Language: Английский
Citations
1
Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 28, 2024
ABSTRACT Heterotrophic microbes rely on host‐derived carbon sources for their growth and survival. Depriving pathogens of plant is therefore a promising strategy protecting plants from disease reducing yield losses. Importantly, this starvation‐mediated resistance expected to be more broad‐spectrum durable than race‐specific R ‐gene‐mediated resistance. Although sugars are well characterized as major bacteria, emerging evidence suggests that plant‐derived lipids likely an essential source some fungal microbes, particularly biotrophs. Here, we comprehensively discuss the dual roles (mainly lipids) transport processes in immune signalling microbial nutrition. We summarize recent findings revealing crucial susceptibility factors at all stages pathogen infection. In particular, potential pathways by which other delivered biotrophs, including protein‐mediated transport, vesicle trafficking autophagy. Finally, highlight knowledge gaps offer suggestions clarifying mechanisms underlie nutrient uptake providing guidance future research application starvation–mediated
Language: Английский
Citations
5
Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 287 - 287
Published: Feb. 14, 2025
Carbohydrate-binding malectin/malectin-like domain-containing proteins (CBMs) represent a newly discovered subclass of lectins that participate in various biological processes across the bacterial, animal, and plant kingdoms. The OsCBM1 gene rice enhances reactive oxygen species (ROS) burst, contributing to drought-stress tolerance. Nonetheless, functions response biotic stress remain poorly understood. In this research, we was activated by Xoo infection, overexpression increased resistance bacterial blight, while suppression its expression shows opposite trend. may influence blight regulating ROS burst SA signaling pathway through RNA-seq analysis. Overexpression content enhanced activities SOD, POD, CAT enzymes, whereas knockdown exhibited genes associated with enzyme activity pathways validated quantitative real-time polymerase chain reaction (qRT-PCR). These results further clarify function resistance, providing references for disease-resistant breeding.
Language: Английский
Citations
0
BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)
Published: July 11, 2024
Abstract Sugars will be eventually effluxed transporters ( SWEETs ) have been confirmed to play diverse physiological roles in plant growth, development and stress response. However, the characteristics functions of SWEET genes Hemerocallis citrina remain unclear poorly elucidated. In this study, whole genome was utilized conduct bioinformatics analysis a total 19 HcSWEET were successfully identified. Analysis physicochemical properties indicated dominant differences among these HcSWEETs . A phylogenetic revealed that proteins can divided into 4 clades ranging from Clade I IV, where within same clade exhibited shared conserved motifs gene structures. Five six exons contained majority genes, which unevenly distributed across 11 chromosomes. The duplication showed presence pairs. Comparative syntenic maps family might present more closed homology monocotyledons than dicotyledons. Cis-acting element key responsiveness various hormones, light, stresses. Additionally, transcriptome sequencing suggested most had relatively higher expression roots, HcSWEET4a significantly up-regulated under salt stress. Overexpression further verified possibility involved response stress, provides novel insights facilitates in-depth studies functional resistance abiotic
Language: Английский
Citations
3
Agronomy, Journal Year: 2024, Volume and Issue: 14(9), P. 1881 - 1881
Published: Aug. 23, 2024
Rice–Xanthomonas oryzae pv. oryzicola (Xoc) is one of the commonly used rice models host–pathogen interactions. Xoc causes bacterial leaf streak (BLS) and has quarantine status. As a Gram-negative pathogen, usually employs type III secretion effectors (T3SEs), including transcription activator-like (TALEs) non-TALEs, to interfere with innate immunity rice. However, few major resistance genes corresponding are found in cultivations; only Rxo1-AvrRxo1 Xo1-TALEs interactions have been discovered rice–Xoc. In this review, we focus on role T3S system (T3SS) virulence consider reported AvrRxo1, AvrBs2, XopN, XopC2, XopAP, XopAK, as well TALEs Tal2g/Tal5d, Tal2h, Tal2a, Tal7, Tal10a, TalI, Tal2b, Tal2c. Interestingly, XopAP disturb stomatal opening promote infection through targeting diverse signaling pathways Otherwise, Tal2b Tal2c, respectively, activate two salicylic acid (SA) hydroxylation redundantly suppress SA-mediated basal defense, which unknown targets, suppresses SA pathway addition, other factors discussed. conclusion, several T3SEs from similar defense achieve invasion, providing an outlook for control disease manipulating conserved pathways.
Language: Английский
Citations
0
Advanced Agrochem, Journal Year: 2024, Volume and Issue: 4(1), P. 78 - 89
Published: Nov. 9, 2024
Language: Английский
Citations
0