Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(6)
Published: June 1, 2024
Language: Английский
aBIOTECH, Journal Year: 2025, Volume and Issue: 6(1), P. 81 - 90
Published: Feb. 10, 2025
Abstract CRISPR/Cas is a simple, robust, versatile tool for plant biology studies and precision breeding. However, establishing high-efficiency gene editing system multiplex of the autotetraploid crop alfalfa ( Medicago sativa L.), most important forage legume worldwide, remains formidable challenge. Here, we systematically identified endogenous U6 promoters in through transient expression via Agrobacterium -mediated infiltration leaves. We further demonstrated efficacy three active genome using an optimized hairy root system. Subsequently, established improved CRISPR/Cas9 containing or four tandemly arrayed MsU6 -promoter-driven polycistronic tRNA-sgRNA (PTG) cassettes, each consisting units, to simultaneously edit genes, coupled with visual reporter RH1 RUBY . This toolkit showed efficient selection. successfully obtained regenerated, red-colored shoots resulting from stable transformation alfalfa. These results highlight potential application Our enables convenient, alfalfa, providing toolset facilitate functional multiple genes families basic research genetic improvement
Language: Английский
Citations
1
PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 196(2), P. 735 - 744
Published: Aug. 8, 2024
Abstract Heterosis refers to the phenomenon where first filial offspring (F1) from genetically diverse parents displays advantages in growth rate, yield, and adaptability compared with its parents. The exploitation of heterosis rice breeding has greatly increased productivity, making a significant contribution food security last half century. Conventional hybrid highly relies on breeder's experience random crossing comprehensive field selection. This process is time-consuming labor-intensive. In recent years, encountered challenges stemming limited germplasm resource, low efficiency, high uncertainty, which constrain progress yield increase, coupled difficulties balancing grain quality, resistance. Understanding genetic basis could lead advancements concepts methods. will fully unleash heterosis. this review, we focus research dissection crop briefly introduce some key modern intelligent hybrid.
Language: Английский
Citations
4
BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)
Published: March 11, 2025
To enhance the cultivation and utility of alfalfa (Medicago sativa) in calcium-rich environments, we assessed germination, growth, physiological responses seven varieties—Crown, Dieter, PANGO, Gladiator, Victoria, WL525, Magnum 801—under varying calcium chloride (CaCl2) concentrations (0, 5, 25, 50 mmol·L−1). Germination indices, root shoot enzyme activities, osmotic regulation parameters were analyzed to evaluate adaptive stress. Our results showed that adapts stress by increasing length, enhancing regulating substance content, reducing malondialdehyde levels, thereby striving maintain stable dry matter content. However, extent these varied among different varieties. Based on a comprehensive evaluation, adaptability varieties ranked following order: Gladiator > Victoria Dieter 801 WL525 Crown PANGO. Notably, 5–25 mmol·L−1 found be optimal for regulation, whereas higher (50 mmol·L−1) induced oxidative impaired growth. This study highlights role exogenous resilience provides robust framework selecting calcium-tolerant suitable karst landscapes. These findings offer theoretical practical insights optimizing forage production soils.
Language: Английский
Citations
0
Theoretical and Applied Genetics, Journal Year: 2024, Volume and Issue: 137(6)
Published: June 1, 2024
Language: Английский
Citations
3