An integrated physiological indicator and transcriptomic analysis reveals the response of soybean buds to high-temperature stress DOI Creative Commons
Jiajia Li, Meiyan Wu,

H. Chen

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 20, 2024

Under global warming, high temperature (HT) has become a major meteorological factor affecting soybean production. To explore the candidate genes and regulatory mechanism of bud response to HT stress, previously identified as HT-tolerant ('Handou14'; HD14) HT-sensitive ('Jiadou36'; JD36) were treated for 5 days in an artificial climate incubator either with (43 °C (day)/ 33 (night), 16 h light/8 darkness) or non-stress growth condition (25 °C, control at stage used experimental materials this study. After treatment, changes physiological indicators including hypocotyl length, enzyme activity hormone content detected; same time, cotyledons, hypocotyls, main roots collected transcriptome sequencing analysis. Analyzing mechanisms stress HD14 JD36 transcription levels. Analysis indicator showed that activities superoxide dismutase (SOD) significantly increased 47.4% 41.2% cotyledon root JD36, contents peroxidase (POD) 61.5% 125% JD36; malonaldehyde (MDA) 44.8% 22.2% after treatment. The abscisic acid (ABA) 1.9 fold 1.2 whereas gibberellin (GA) decreased 2.2 1.3 root, 1.6 (P < 0.05). Thus, higher SOD POD activities, ABA content, smaller increase MDA may improve tolerance stress. cultivar have stronger GA signal transduction combat negative effects HT. RNA-sequencing was performed analyze differential expression buds two cultivars under treatment condition. In total, 3,633, 1,964, 9,934, 3,036 differentially expressed (DEGs) CH (control group vs. TH (HT-treatment HD14), CJ TJ JD36), TH, comparison groups, respectively. Bioinformatic analysis revealed most DEGs mainly involved metabolic processes, catalytic activity, carbohydrate, energy transduction, signaling pathways. results qRT-PCR validation (86.67%) consistent data. Five selected stage. summary, cells are protected from oxidative damage by antioxidant accumulation Concomitantly, crucial transmission processes induced, thus initiating adaptive protective mechanisms. This study provides theoretical basis clarification molecular bud.

Language: Английский

Sensitivity to Photoperiod Is a Complex Trait in Camelina sativa DOI Creative Commons
Bryan Ramirez-Corona, Eric A. Seagren, Carissa Sherman

et al.

Plant Direct, Journal Year: 2025, Volume and Issue: 9(4)

Published: April 1, 2025

ABSTRACT Day neutrality, or insensitivity to photoperiod (day length), is an important domestication trait in many crop species. Although the oilseed C. sativa has been cultivated since Neolithic era, day‐neutral accessions have yet be described. We sought leverage genetic diversity existing germplasms identify with low sensitivity for future engineering of this trait. To do so, we quantified variation hypocotyl length across 161 4‐day‐old seedlings grown long‐day and short‐day conditions as a high‐throughput approximation response. Soil‐grown adult plants from selected also showed response day several traits; however, responses seedling traits were not correlated, suggesting complex mechanistic underpinnings. RNA‐seq experiments reference accession Licalla identified differentially regulated Arabidopsis syntelogs involved development, including COL2 , FT LHY WOX4, expression these genes did correlate differences their sensitivity. Taken together, show that all tested some degree likely complex, involving separable traits.

Language: Английский

Citations

0
Identification of R2R3-MYB Gene Family and Functional Analysis of Responses of S22 Subfamily to Abiotic Stresses in Dandelion (Taraxacum mongolicum Hand.-Mazz.) DOI Open Access

L.Z. Lu,

Songle Fan, Bi Qin

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3422 - 3422

Published: April 5, 2025

Dandelions possess a wide range of medicinal properties and demonstrate remarkable adaptability tolerance to salinity alkalinity. MYB genes in plants are implicated growth, differentiation, metabolism, responses both biotic abiotic stresses. The function dandelions, particularly the R2R3-MYB gene family, requires further investigation. In this study, we identified total 130 members dandelion family at genome-wide level, all which were mapped eight chromosomes. MEME analysis revealed that TmR2R3-MYB proteins contain three conserved motifs. Phylogenetic categorized TmR2R3-MYBs into 29 subfamilies. Transcriptomic studies different tissues indicated exhibit distinct expression patterns tissues, indicating their diverse functions dandelions. Notably, TmMYB44 from S22 subfamily displayed highest level roots. Additionally, six representative selected for profiling under alkalinity treatments. results demonstrated involved response stress. These findings provide basis exploration stress tolerance.

Language: Английский

Citations

0
A comprehensive analysis of MYB aimed at identifying genes involved in the regulation of natural rubber biosynthesis DOI Creative Commons
Songle Fan,

L.Z. Lu,

Xiaokang Fan

et al.

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 230, P. 121114 - 121114

Published: May 5, 2025

Language: Английский

Citations

0
Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids DOI Creative Commons

Yunxiao Guan,

Qiaoyu Zhang, Ting Zhang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 24, 2025

Phalaenopsis orchids, belonging to the Orchidaceae family, one of largest groups angiosperms, possess significant commercial value due their fascinating flowers. Petal size is a vital trait that directly determines flower and shape Phalaenopsis. However, genetic developmental regulation petal in remains unexplored. In this study, we tracked growth pattern through five stages opening, discovering cell division stops at stage 2 3 5 are critical periods rapid expansion. RNA-seq was then conducted further reveal molecular mechanisms underlying regulation. Gene ontology (GO) analysis indicated differentially expressed genes (DEGs) from four comparable were both enriched terms related Endogenous hormone assays showed auxin, cytokinin, gibberellin implicated Moreover, six auxin signaling pathway genes, 11 expansion-related 30 transcription factors (TFs) identified trend abundantly during period expansion, suggesting they may influence by regulating contrast, 18 TFs exhibited highest expression levels S1 stage, indicating potential role proliferation. Based on weighted gene co-expression network (WGCNA), hub (PaLOF2, PaSWEET11, PaVNI2, PaHDA3, PaPMEI3, PaXTH30) screened green yellow module which highly associated with size. Our results lay foundation for exploration development breeding programs aimed generating novel desirable traits.

Language: Английский

Citations

0
Soybean Oil and Protein: Biosynthesis, Regulation and Strategies for Genetic Improvement DOI
Hui Li,

Jia Sun,

Ying Zhang

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 24, 2024

ABSTRACT Soybean ( Glycine max [L.] Merr.) is one of the world's most important sources oil and vegetable protein. Much energy required for germination early growth soybean seeds stored in fatty acids, mainly as triacylglycerols (TAGs), main seed storage proteins are β‐conglycinin (7S) glycinin (11S). Recent research advances have deepened our understanding biosynthetic pathways transcriptional regulatory networks that control acid protein synthesis organelles such plastid, ribosome endoplasmic reticulum. Here, we review composition oils proteins, summarizing key enzymes transcription factors recently been shown to regulate synthesis/metabolism. We then discuss newest genomic strategies manipulating these genes increase food value soybeans, highlighting priorities future genetic improvement this staple crop.

Language: Английский

Citations

2
Role of BraSWEET12 in Regulating Flowering through Sucrose Transport in Flowering Chinese Cabbage DOI Creative Commons

Qinqin He,

Li-Ming He,

Zongqin Feng

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(10), P. 1037 - 1037

Published: Sept. 29, 2024

We assessed the flowering Chinese cabbage (Brassica rapa var. parachinensis), a specialty vegetable found in southern China. The sugar content of stem tip is closely related to bolting and flowering. Sugar Will Eventually be Exported Transporters (SWEETs) are bidirectional transporter proteins involved numerous plant growth development processes. expression BraSWEET12 positively correlated with content. However, it unclear whether In this study, we identified characterized BraSWEET12. contains 288 amino acids located on cell membrane as sucrose protein. highly expressed petals tips upregulated by gibberellin low temperatures. Overexpression Arabidopsis can increase at tip, upregulate AtAP1 AtLFY, advance time. Subsequently, our results indicate that accumulation plays crucial role regulation. These provide reference for elucidating regulatory mechanisms underlying

Language: Английский

Citations

0
An integrated physiological indicator and transcriptomic analysis reveals the response of soybean buds to high-temperature stress DOI Creative Commons
Jiajia Li, Meiyan Wu,

H. Chen

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 20, 2024

Under global warming, high temperature (HT) has become a major meteorological factor affecting soybean production. To explore the candidate genes and regulatory mechanism of bud response to HT stress, previously identified as HT-tolerant ('Handou14'; HD14) HT-sensitive ('Jiadou36'; JD36) were treated for 5 days in an artificial climate incubator either with (43 °C (day)/ 33 (night), 16 h light/8 darkness) or non-stress growth condition (25 °C, control at stage used experimental materials this study. After treatment, changes physiological indicators including hypocotyl length, enzyme activity hormone content detected; same time, cotyledons, hypocotyls, main roots collected transcriptome sequencing analysis. Analyzing mechanisms stress HD14 JD36 transcription levels. Analysis indicator showed that activities superoxide dismutase (SOD) significantly increased 47.4% 41.2% cotyledon root JD36, contents peroxidase (POD) 61.5% 125% JD36; malonaldehyde (MDA) 44.8% 22.2% after treatment. The abscisic acid (ABA) 1.9 fold 1.2 whereas gibberellin (GA) decreased 2.2 1.3 root, 1.6 (P < 0.05). Thus, higher SOD POD activities, ABA content, smaller increase MDA may improve tolerance stress. cultivar have stronger GA signal transduction combat negative effects HT. RNA-sequencing was performed analyze differential expression buds two cultivars under treatment condition. In total, 3,633, 1,964, 9,934, 3,036 differentially expressed (DEGs) CH (control group vs. TH (HT-treatment HD14), CJ TJ JD36), TH, comparison groups, respectively. Bioinformatic analysis revealed most DEGs mainly involved metabolic processes, catalytic activity, carbohydrate, energy transduction, signaling pathways. results qRT-PCR validation (86.67%) consistent data. Five selected stage. summary, cells are protected from oxidative damage by antioxidant accumulation Concomitantly, crucial transmission processes induced, thus initiating adaptive protective mechanisms. This study provides theoretical basis clarification molecular bud.

Language: Английский

Citations

0