Natural variation in promoters of F3′5′H and ANS correlates with catechins diversification in Thea species of genus Camellia DOI

Yanrui Zhang,

Haiyan Pan,

Qiong Wu

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Catechins were diversely accumulated in Thea plants and crucial for tea flavor, yet the mechanism underlying diverse catechins distribution remained elusive. We herein collected a total of 19 12 non‐ to investigate their mechanism. Results showed that pattern cultivated significantly differs from wild relatives. (+)‐Gallocatechin gallate (GCG) was detected over 50% but almost undetectable plants. Conversely, (−)‐Epigallocatechin (EGCG) extensively distributed cultivars extremely low few relatives such as Camellia tetracocca C. ptilophylla . Expression analysis found expression flavonoid 3′,5′‐hydroxylase ( F3′5′H ) highly correlated with EGCG accumulation Yeast one‐hybrid luciferase assays CsMYB1, key regulator, could bind promoter activate its promote plants; it unable due 14‐bp deletion promoter, leading content EGCG. also silencing anthocyanidin synthase ANS enhanced metabolic flux toward GCG not plants, consistent observation high expression. Overall, results illustrated variation would help facilitate utilization future breeding.

Language: Английский

Role of jasmonates in plant response to temperature stress DOI

Aamira Iqbal,

He‐Nan Bao,

Jian Wang

et al.

Plant Science, Journal Year: 2025, Volume and Issue: unknown, P. 112477 - 112477

Published: March 1, 2025

Language: Английский

Citations

0
Natural variation in promoters of F3′5′H and ANS correlates with catechins diversification in Thea species of genus Camellia DOI

Yanrui Zhang,

Haiyan Pan,

Qiong Wu

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(6)

Published: March 1, 2025

SUMMARY Catechins were diversely accumulated in Thea plants and crucial for tea flavor, yet the mechanism underlying diverse catechins distribution remained elusive. We herein collected a total of 19 12 non‐ to investigate their mechanism. Results showed that pattern cultivated significantly differs from wild relatives. (+)‐Gallocatechin gallate (GCG) was detected over 50% but almost undetectable plants. Conversely, (−)‐Epigallocatechin (EGCG) extensively distributed cultivars extremely low few relatives such as Camellia tetracocca C. ptilophylla . Expression analysis found expression flavonoid 3′,5′‐hydroxylase ( F3′5′H ) highly correlated with EGCG accumulation Yeast one‐hybrid luciferase assays CsMYB1, key regulator, could bind promoter activate its promote plants; it unable due 14‐bp deletion promoter, leading content EGCG. also silencing anthocyanidin synthase ANS enhanced metabolic flux toward GCG not plants, consistent observation high expression. Overall, results illustrated variation would help facilitate utilization future breeding.

Language: Английский

Citations

0