Functional Characterization of Pomegranate CAMTA3 in Cold Stress Responses

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 813 - 813

Published: March 5, 2025

Cold stress is a significant factor limiting plant growth and development. Pomegranate particularly susceptible to low temperatures. Calmodulin-binding transcriptional activators (CAMTAs) are key regulators of cold tolerance in plants. In this study, we conducted comprehensive analysis the CAMTA family proteins across 12 species, including Punica granatum (pomegranate), using bioinformatic methods. CAMTA3 (PgCAMTA3) was isolated characterized, it demonstrated enhanced when expressed Arabidopsis thaliana. Quantitative real-time PCR (qRT-PCR) showed that expression PgCAMTA3 up-regulated under ABA treatments pomegranates. Two A. thaliana transgenic lines, OE1 OE2, which overexpress PgCAMTA3, were generated through genetic transformation. The overexpression OE2 exhibited higher survival rates stress. Furthermore, enzymatic activity assays revealed peroxidase (POD), catalase (CAT), superoxide dismutase (SOD) OE lines. These antioxidant activities collectively contribute better by providing more effective reactive oxygen species (ROS) scavenging cellular protection mechanisms, confirmed lower levels malondialdehyde (MDA) ROS production. addition, led upregulation AtCBF2, AtNCED3, AtWRKY22, modulated CAMTA3. summary, report role tolerance. Our findings provide valuable insights into CAMATA plants offer new perspectives on molecular mechanisms underlying

Language: Английский

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Functional characterization of calmodulin-like proteins, CML13 and CML14, as novel light chains of Arabidopsis class VIII myosins

Journal of Experimental Botany, Journal Year: 2024, Volume and Issue: 75(8), P. 2313 - 2329

Published: Jan. 27, 2024

Myosins are important motor proteins that associate with the actin cytoskeleton. Structurally, myosins function as heteromeric complexes where smaller light chains, such calmodulin (CaM), bind to isoleucine-glutamine (IQ) domains in neck region facilitate mechano-enzymatic activity. We recently identified Arabidopsis CaM-like (CML) CML13 and CML14 interactors of containing multiple IQ domains, including a myosin VIII. Here, we demonstrate CaM, CML13, all four VIII isoforms. Among CMLs tested for binding VIIIs, gave strongest signals using planta split-luciferase protein interaction assays. In vitro, recombinant showed specific, high-affinity, calcium-independent VIIIs. co-localized plasma membrane-bound puncta when co-expressed red fluorescent protein-myosin fusion tail vitro motility assays VIIIs demonstrated chains. Suppression or expression RNA silencing resulted shortened-hypocotyl phenotype, similar observed quadruple mutant, viii4KO. Collectively, our data indicate novel

Language: Английский

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Early signaling enhance heat tolerance in Arabidopsis through modulating jasmonic acid synthesis mediated by HSFA2

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 267, P. 131256 - 131256

Published: March 29, 2024

Language: Английский

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The VAMP-associated protein VAP27-1 plays a crucial role in plant resistance to ER stress by modulating ER–PM contact architecture in Arabidopsis

Plant Communications, Journal Year: 2024, Volume and Issue: 5(7), P. 100929 - 100929

Published: April 27, 2024

The endoplasmic reticulum (ER) and the plasma membrane (PM) form ER–PM contact sites (EPCSs) that allow ER PM to exchange materials information. Stress-induced disruption of protein folding triggers stress, cell initiates unfolded response (UPR) resist stress. However, whether EPCSs play a role in stress plants remains unclear. VESICLE-ASSOCIATED MEMBRANE PROTEIN (VAMP)-ASSOCIATED 27-1 (VAP27-1) functions EPCS tethering is encoded by family 10 genes (VAP27-1–10) Arabidopsis thaliana. Here, we used CRISPR-Cas9-mediated genome editing obtain homozygous vap27-1 vap27-3 vap27-4 (vap27-1/3/4) triple mutant lacking three key VAP27 members Arabidopsis. vap27-1/3/4 exhibits defects connectivity architecture, as well excessive UPR signaling. We further showed relocation VAP27-1 mediates specific VAP27-1-related remodeling expansion under Moreover, spatiotemporal dynamics at increase enhance resistance In addition, revealed an important for intracellular calcium homeostasis regulation Taken together, these results broaden our understanding molecular cellular mechanisms signaling plants, providing additional clues improving plant broad-spectrum different stresses.

Language: Английский

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Calmodulin: Coping with biotic and abiotic stresses in soybean (Glycine max (L.) Merr.)

Plant Stress, Journal Year: 2024, Volume and Issue: unknown, P. 100602 - 100602

Published: Sept. 1, 2024

Language: Английский

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Comprehensive genome-wide characterization of calmodulin-binding protein 60 gene family reveals PtCBP60E1 enhances the pathogen resistance in poplar

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120601 - 120601

Published: Feb. 5, 2025

Language: Английский

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Calmodulin-Binding Transcription Factors: Roles in Plant Response to Abiotic Stresses

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 532 - 532

Published: Feb. 10, 2025

Plants face many abiotic stresses throughout their life cycle, such as drought, high temperature, low and salinity. To survive reproduce, plants have evolved a complex elaborate signal transduction network to sense stress signals initiate corresponding defense mechanisms. Calcium ion (Ca2+), secondary messenger, plays an important role in mediating plant cells. Calmodulin (CaM) is class of Ca2+ receptors that changes cellular calcium concentration can interact with range proteins regulate the activity downstream target proteins. Calmodulin-binding transcription factors (CAMTAs) are family (TFs) widely present bind CaM. The CAMTAs regarded most characterized CaM-binding TF signaling pathway. In recent years, studies shown play regulatory response growth development. Therefore, this review summarizes progress discovery, structure, under stresses, view providing reference for future CAMTA studies. Finally, prospects directions further research on potential mechanisms also discussed.

Language: Английский

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Genetic diversity of Cucurbita maxima accessions representing the Eastern European gene pool

Genetic Resources and Crop Evolution, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 17, 2025

Language: Английский

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Calmodulin and calcium signaling in potato tuberization: The role of membrane transporters in stress adaptation

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109829 - 109829

Published: March 1, 2025

Language: Английский

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Interaction between pathogenesis‐related (PR) proteins and phytohormone signaling pathways in conferring disease tolerance in plants

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Pathogenesis-related (PR) proteins are critical defense signaling molecules induced by phytopathogens. They play a vital role in plant's pathways and innate immunity, particularly systemic acquired resistance (SAR) serve as key molecular markers of plant defense. Overexpressing PR genes, such chitinase, thaumatin, glucanase, thionin defensin, either individually or combination, have significantly boosted plants' responses against various pathogens. However, regulating the expression these versatile remain only partially understood. Plant hormones like salicylic acid (SA) jasmonic (JA) known for their well-established roles gene to pathogens other stress conditions. genes interact with components hormonal pathways, including receptors (e.g., NPR1 SA signaling), transcription factors MYC2 JA cis-regulating elements W-box), modulate responses. Recent studies highlighted contributions different immunity interactions process crosstalk, which helps coordinate activation. This review provides comprehensive overview proteins, complexity, crosstalk aiming understand improved pathogen resistance.

Language: Английский

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