Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Dec. 19, 2023

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Vacuolar Degradation of Plant Organelles DOI
Marisa S. Otegui, Charlotte Steelheart, Wenlong Ma

et al.

The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3036 - 3056

Published: April 24, 2024

Plants continuously remodel and degrade their organelles due to damage from metabolic activities environmental stressors, as well an integral part of cell differentiation programs. Whereas certain use local hydrolytic enzymes for limited remodeling, most the pathways that control partial or complete dismantling rely on vacuolar degradation. Specifically, selective autophagic play a crucial role in recognizing sorting plant organelle cargo clearance, especially under cellular stress conditions induced by factors like heat, drought, damaging light. In these short reviews, we discuss mechanisms degradation chloroplasts, mitochondria, endoplasmic reticulum, Golgi, peroxisomes, with emphasis autophagy, recently discovered autophagy receptors organelles, crosstalk other catabolic pathways.

Language: Английский

Citations

7
Metabolic strategies in hypoxic plants DOI Creative Commons
Hans van Veen, Paolo M. Triozzi, Elena Loreti

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 24, 2024

Abstract Complex multicellular organisms have evolved in an oxygen-enriched atmosphere. Oxygen is therefore essential for all aerobic organisms, including plants, energy production through cellular respiration. However, plants can experience hypoxia following extreme flooding events and also under aerated conditions proliferative organs or tissues characterized by high oxygen consumption. When availability compromised, adopt different strategies to cope with limited aeration. A common feature among plant species the activation of anaerobic fermentative metabolism provide ATP maintain homeostasis hypoxia. Fermentation requires many sugar substrates, which not always feasible, alternative metabolic are thus needed. Recent findings shown that hypoxic active specific conditions. Here, we describe regulatory mechanisms control how they enable them thrive despite challenging comprehensive mechanistic understanding genetic physiological components underlying should help opportunities improve resilience current climate change scenario.

Language: Английский

Citations

2
Vitexin is a potential postharvest treatment for ameliorating litchi fruit pericarp browning by regulating autophagy DOI

Qiuxiao Yang,

Dingding Shi,

Yan-Ling Ren

et al.

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 216, P. 113061 - 113061

Published: June 19, 2024

Language: Английский

Citations

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 7, 2024

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Citations

1
Autophagy Regulates Plant Tolerance to Submergence by Modulating Photosynthesis DOI
Mingkang Yang,

Junyu Wei,

Yarou Xu

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 22, 2024

ABSTRACT The increase in global climate variability has increased the frequency and severity of floods, profoundly affecting agricultural production food security worldwide. Autophagy is an intracellular catabolic pathway that dispensable for plant responses to submergence. However, physiological role autophagy response submergence remains unclear. In this study, a multi‐omics approach was applied by combining transcriptomics, proteomics, lipidomics characterize molecular changes Arabidopsis autophagy‐defective mutant ( atg5‐1 ) responding Our results revealed resulted remarkable transcriptome, proteome, lipidome . Under submerged conditions, levels chloroplastidic lipids, including monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), phosphatidylglycerol (PG), were lower than wild‐type, suggesting may affect photosynthesis regulating lipid metabolism. Consistently, photosynthesis‐related proteins photosynthetic efficiency decreased under conditions. Phenotypic analysis inhibition tolerance Compared wild‐type plants, plants showed significant decrease starch content after Collectively, our findings reveal novel via regulation underwater content.

Language: Английский

Citations

1
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Creative Commons
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 14, 2023

Abstract Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Citations

1
Osmotic stress in roots drives lipoxygenase-dependent plastid remodeling through singlet oxygen production DOI Open Access
Dekel Cohen-Hoch, Tomer Chen, Lior Sharabi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Abstract Osmotic stress, caused by the lack of water or high salinity, is a common environmental problem in roots. stress can be reproducibly simulated with application solutions high-molecular-weight and impermeable polyethylene glycol. Different reactive oxygen species such as singlet oxygen, superoxide hydrogen peroxide accompany this stress. Among them, produced byproduct lipoxygenase activity, was shown to associated limiting root growth. To better understand source effect its production followed at cellular level. initiated profound changes plastid morphology vacuole structure. By confocal electron microscopy plastids were accompanied appearance multiple small extraplastidic bodies that also an intense oxygen. A marker protein, CRUMPLED LEAF, indicated these originated from outer membrane. Remarkably type 9 lipoxygenase, LOX5, change distribution uniformly cytoplasmic more clumped together bodies. In addition, oxylipin products increased while 13 lipoxygenases decreased. Inhibition SHAM inhibitor down-regulated lines prevented cells initiating responses leading cell death. contrast, scavenging halted terminal These findings underscore reversible nature osmotic stress-induced changes, emphasizing pivotal roles physiology.

Language: Английский

Citations

0
Mediator Subunit 17 Regulates Light and Dark Responses in Arabidopsis Plants DOI
Marisol Giustozzi,

Santiago Nicolás Freytes,

Marı́a Lorena Falcone Ferreyra

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Autophagosome development and chloroplast segmentation occur synchronously for piecemeal degradation of chloroplasts DOI Open Access
Masanori Izumi, Sakuya Nakamura, Kohei Otomo

et al.

Published: Sept. 11, 2024

Plants distribute many nutrients to chloroplasts during leaf development and maturation. When leaves senesce or experience sugar starvation, the autophagy machinery degrades chloroplast proteins facilitate efficient nutrient reuse. Here, we report on intracellular dynamics of an pathway responsible for piecemeal degradation components. Through live-cell monitoring morphology, observed formation budding structures in sugar-starved leaves. These buds were then released incorporated into vacuolar lumen as autophagic cargo termed a Rubisco-containing body. The did not accumulate mutants core machinery, suggesting that autophagosome creation is required forming buds. Simultaneous tracking morphology revealed isolation membranes autophagosomes interact closely with part surface before Chloroplasts protrude at site associated membranes, which divide synchronously This autophagy-related division does require DYNAMIN-RELATED PROTEIN 5B, constitutes ring proliferation growing An unidentified may thus fragment coordination chloroplast-associated membrane.

Language: Английский

Citations

0
Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants DOI
Marisol Giustozzi,

Santiago Nicolás Freytes,

Marı́a Lorena Falcone Ferreyra

et al.

Plant Science, Journal Year: 2024, Volume and Issue: unknown, P. 112285 - 112285

Published: Oct. 1, 2024

Language: Английский

Citations

0