Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments DOI Creative Commons
Marcela T. Miranda, Luciano Pereira, Gabriel S. Pires

et al.

Tree Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Considerable attention has been paid to addressing methodological concerns related measurements of embolism in conduits angiosperm xylem. A fast, easy and cheap method is based on gas extraction from dehydrating samples obtain pneumatic vulnerability curves (VCs). Here, we tested the assumption that cutting open leads gas-filled lumina when these are cut air at fairly high water potentials, which required detect intact conduits. We performed VCs with Pneumatron for 12 species extracted sap cut-open vessels branches nine under early stages branch dehydration. The optical was applied Citrus plants as an alternative reference estimate resistance. found increase discharge during dehydration, affected most studied. Xylem residue not absorbed immediately by surrounding tissue six but gradually disappeared over time progressive amount discharged increased until all residual absorbed, embolism. conclude xylem affects represents a novel artifact can easily be corrected for. Yet, it remains unclear why exactly air–water meniscus did fully withdraw conduit end wall species. By analyzing slope time, could improve estimations resistance, evidenced strong agreement between methods. Since some slightly underestimate propose apply correction this time-resolution taken Pneumatron.

Language: Английский

Drought tolerance: a perspective about leaf venation and the role of auxin DOI
Mariana Rocha de Carvalho, Elisa Monteze Bicalho, Eduardo Gusmão Pereira

et al.

Theoretical and Experimental Plant Physiology, Journal Year: 2025, Volume and Issue: 37(1)

Published: Jan. 22, 2025

Language: Английский

Citations

1
Response to Water Stress of Eight Novel and Widely Spread Citrus Rootstocks DOI Creative Commons
Giulia Modica,

Fabio Arcidiacono,

Ivana Puglisi

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(5), P. 773 - 773

Published: March 3, 2025

Drought is a problematic abiotic stress affecting citrus crops in the Mediterranean basin and rootstock plays fundamental role adopting adaptive mechanisms response to water deficit. The aim of this study evaluate eight rootstocks under three treatments imposed: control (100% reference evapotranspiration, Et0), 66% Et0 50% Et0. genotypes studied were C35 citrange, Bitters, Carpenter Furr which have been recently spread so far, little investigated, while others widely used especially industry, i.e., Citrus macrophylla, C. volkameriana, Swingle citrumelo Carrizo citrange. Morphological analyses, leaf chlorophyll content determination, physiological measurement, proline accumulation, malondialdehyde determination antioxidant enzyme activities measured. results exhibited that Bitters showed an increment area reduce effects drought conditions. A decrement gas exchanges xylem potential was noticed citrange at both shortage treatments. significant increase stresses (90.3 103.3%, for 66 Et0). performed better than other with regard enzymatic hormonal assays. Specifically, reduction CAT (−68.6%), SOD (−82.5%) APX (−36.7%). Furthermore, closely related morphological parameters, e.g., root length, measurements. volkameriana decrease potential, overall citranges susceptible reducing

Language: Английский

Citations

1
Revealing Drought Tolerance Strategies in Pistachio Clonal Hybrids: Role of Osmotic Adjustment DOI Creative Commons

Mozhdeh Osku,

Mahmoud Reza Roozban, Saadat Sarikhani

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Abstract Background Pistachio (Pistacia vera L.) growth, yield and quality are affected by abiotic stress especially drought. Understanding the strategies that improve dehydration tolerance is essential for developing resistant pistachio rootstocks. In experiment, nine-month-old saplings of seven clonal interspecies hybrids Pistacia atlantica × P. integerrima (C1, C2, C16-1, C8-3, C4-2, C9-4 UCB1) were assessed growth physiological responses to water withholding recovery. Result Water deficit negatively impacted parameters, including shoot dry weight, root weight leaf area, in all hybrids; however, C1 demonstrated relatively minor reductions compared other hybrids. Glycine betaine content leaves increased 49.4% 47% C1, while only 7% 11% increases found most sensitive clones, C8-3 C4-2. Notably, C9-4, identified as tolerant clone, displayed highest proline levels, with 29.5% 41.5% roots, contrast which showed minimal 6% respectively. Clones higher compatible solutes maintained relative (RWC), lower osmotic potential smaller potential. RWC declined just whereas it dropped 88% C8-3. Osmotic potentials − 1.61 MPa 0.271 they 0.93 0.11 Following recovery, evaluations traits visual observations indicated had poor recovery ability. Heatmap PCA analyses categorized clones into three groups: "tolerant" (C9-4, C2), "moderately tolerant" (UCB1) "sensitive" (C8-3, C4-2 C16-1). Conclusion The results this study underscore significance adjustment a more critical trait stomatal parameters effectively differentiating from ones.

Language: Английский

Citations

0
Revealing drought tolerance strategies in pistachio clonal hybrids: role of osmotic adjustment DOI Creative Commons

Mozhdeh Osku,

Mahmoud Reza Roozban, Saadat Sarikhani

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 2, 2025

Pistachio (Pistacia vera L.) growth, yield and quality are affected by abiotic stress especially drought. Understanding the strategies that improve dehydration tolerance is essential for developing resistant pistachio rootstocks. In experiment, nine-month-old saplings of seven clonal interspecies hybrids Pistacia atlantica × P. integerrima (C1, C2, C16-1, C8-3, C4-2, C9-4 UCB1) were assessed growth physiological responses to water withholding recovery. Water deficit negatively impacted parameters, including shoot dry weight, root weight leaf area, in all hybrids; however, C1 demonstrated relatively minor reductions compared other hybrids. Glycine betaine content leaves increased 49.4% 47% C1, while only 7% 11% increases found most sensitive clones, C8-3 C4-2. Notably, C9-4, identified as tolerant clone, displayed highest proline levels, with 29.5% 41.5% roots, contrast which showed minimal 6% respectively. Clones higher compatible solutes maintained relative (RWC), lower osmotic potential smaller potential. RWC declined just whereas it dropped 88% C8-3. Osmotic potentials - 1.61 MPa 0.271 they 0.93 0.11 Following recovery, evaluations traits visual observations indicated had poor recovery ability. Heatmap PCA analyses categorized clones into three groups: "tolerant" (C9-4, C2), "moderately tolerant" (UCB1) "sensitive" (C8-3, C4-2 C16-1). The results this study underscore significance adjustment a more critical trait stomatal parameters effectively differentiating from ones.

Language: Английский

Citations

0
Adaptation in Wood Anatomical Traits to Temperature and Precipitation—A Common Garden Study DOI Creative Commons

Tian-Tian PAN,

Travis G. Britton, Julian Schrader

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

ABSTRACT Indisputably, temperature and precipitation are key environmental variables driving plant trait variation shaping ecological strategies. However, it is challenging to ascertain their relative influences because site often correlated. Here, using Eucalyptus as a model system representing woody evergreen species more broadly, we sought disentangle influence on wood anatomical traits underpinning hydraulics. From common garden sampled 29 pairs of closely‐related species, each species‐pair either contrast in or precipitation, but never both. Very clearly, both phylogenetic non‐phylogenetic analyses, from lower‐rainfall colder regions had thicker vessel walls, likely an adaptation drought freezing, enabling water transport at negative potentials with reduced risk cavitation implosion. On average, warmer smaller vessels, theoretical hydraulic conductivity remained stable across temperatures due increased density compensating for diameters. These trends being observed adult plants grown under conditions suggests that anatomy “hard‐wired”, gene × environment interactions relatively weak. This insight understanding the trait‐basis strategies related climate.

Language: Английский

Citations

0
Xylem sap residue in cut-open conduits can affect gas discharge in pneumatic experiments DOI Creative Commons
Marcela T. Miranda, Luciano Pereira, Gabriel S. Pires

et al.

Tree Physiology, Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Abstract Considerable attention has been paid to addressing methodological concerns related measurements of embolism in conduits angiosperm xylem. A fast, easy and cheap method is based on gas extraction from dehydrating samples obtain pneumatic vulnerability curves (VCs). Here, we tested the assumption that cutting open leads gas-filled lumina when these are cut air at fairly high water potentials, which required detect intact conduits. We performed VCs with Pneumatron for 12 species extracted sap cut-open vessels branches nine under early stages branch dehydration. The optical was applied Citrus plants as an alternative reference estimate resistance. found increase discharge during dehydration, affected most studied. Xylem residue not absorbed immediately by surrounding tissue six but gradually disappeared over time progressive amount discharged increased until all residual absorbed, embolism. conclude xylem affects represents a novel artifact can easily be corrected for. Yet, it remains unclear why exactly air–water meniscus did fully withdraw conduit end wall species. By analyzing slope time, could improve estimations resistance, evidenced strong agreement between methods. Since some slightly underestimate propose apply correction this time-resolution taken Pneumatron.

Language: Английский

Citations

0