Transcriptomic, metabonomic and proteomic analyses reveal that terpenoids and flavonoids are required for Pinus koraiensis early defence against Bursaphelenchus xylophilus infection

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 12, 2025

Abstract Pine wilt disease (PWD), caused by the pine wood nematode (PWN) Bursaphelenchus xylophilus , threatens Pinus seriously. koraiensis is one of most important species in China and host for PWN. However, our understanding defence-regulating process following infection B. at molecular level remains limited. To understand mechanisms that P. responds to invasion, was inoculated with solutions observed no obvious symptoms during early stage; began appear 5 dpi. Therefore, we conducted comparative transcriptomic, metabonomic proteomic analyses between 5dpi 0 In infected plants, 1574 genes were significantly up-regulated, including 17 terpenoid-, 41 phenylpropanoid- 22 flavonoid-related genes. According GO KEGG enrichment up-regulated genes, 86 terms 16 pathways enriched. Most associated phenylpropanoid-, flavonoid- carbohydrate-related events. Similarly, abundance 36 30 metabolites, increased positive negative polarity modes, respectively. Among them, naringenin 3-methyl-2-oxovaleric acid exhibited significant toxic effects on . functional analysis enriched above pathways, addition alkaloid biosynthesis. Although few proteins changed, response stress term proteins. Furthermore, plant receptor-like serine/threonine kinases, pectin methylation modulators, pinosylvin O-methyltransferase arabinogalactan/proline-rich compared healthy plants. These not abundant plant. Overall, these results indicate can actively PWN via various defense strategies, events related terpenoids, flavonoids, phenylpropanoids, lipids alkaloids. Particularly, terpenoids flavonoids are required defence against infection.

Language: Английский

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Response of Pedunculate Oak (Quercus robur L.) to Adverse Environmental Conditions in Genetic and Dendrochronological Studies

Plants, Journal Year: 2025, Volume and Issue: 14(1), P. 109 - 109

Published: Jan. 2, 2025

Pedunculate oak (Quercus robur L.) is widely distributed across Europe and serves critical ecological, economic, recreational functions. Investigating its responses to stressors such as drought, extreme temperatures, pests, pathogens provides valuable insights into capacity adapt climate change. Genetic dendrochronological studies offer complementary perspectives on this adaptability. Tree-ring analysis (dendrochronology) reveals how Q. has historically responded environmental stressors, linking growth patterns specific conditions drought or temperature extremes. By examining tree-ring width, density, dynamics, researchers can identify periods of suppression enhancement predict forest future climatic events. further complement by uncovering adaptive genetic diversity inheritance patterns. Identifying markers associated with stress tolerance enables managers prioritize the conservation populations higher potential. These guide reforestation efforts support development climate-resilient populations. integrating data, gain a holistic understanding robur’s mechanisms resilience. This knowledge vital for management sustainable planning in face challenges, ultimately helping ensure long-term viability their ecosystems. The topics covered review are very broad. We tried include most relevant, important, significant studies, but focused mainly relatively recent Eastern European because they species’ area. However, although more than 270 published works have been cited review, we have, course, missed some studies. apologize advance authors those relevant that not cited.

Language: Английский

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Insect Resistance in Quercus robur: A Comparative Metabolomic and Transcriptomic Analysis of Normal and Curly Leaf Varieties

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Quercus robur is a tree species that produces acorns with rich nutritional value. Its leaf rolling crucial for adapting to stress, but this trait's impact on plant-insect interactions remains unknown. We compared the resistance phenotypes, metabolomic profiles, and transcriptomic data of curly (Qr-T) normal-leaf (Qr-S) varieties Q. robur. Findings revealed Qr-T exhibited higher area consumption under herbivory. Metabolomic analysis found lower levels key defensive compounds like Questiomycin A, Caffeine, Indoleacrylic acid in Qr-T. Transcriptomics up-regulation DEGs related development (e.g., MYO17, LEC2) down-regulation defense-related (like IOS1, Y3471) GO KEGG analyses indicated defense responses isoflavonoid biosynthesis pathway were suppressed Coexpression network identified coordinated genes, such as CYP81Q32 CYP94A5, their coexpressed transcription factors (such WRKY6, WRKY53) The above findings suggest curling may weaken its insect resistance, which provides clues improving plant agroforestry.

Language: Английский

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Protoplast Cell‐Wall Regeneration: Unlocking New Potential for Genetic Improvement and Tree Breeding

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Summary statement The recalcitrance of cell‐wall regeneration in woody plant protoplasts hinders the application protoplast genetic transformation plant. Establishing an efficient system for is a key strategy promoting tree improvement and breeding.

Language: Английский

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A retinoblastoma‐related protein promotes adventitious root development and secondary wall formation in Populus through the SHR/SCR network

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(2)

Published: April 1, 2025

SUMMARY Retinoblastoma‐Related (RBR) proteins, evolutionarily conserved homologs of animal RB tumor suppressor, are involved in cell cycle regulation, differentiation, and stress responses. This study systematically investigates the functional characterization PeRBR hybrid poplar ( Populus deltoides × P. euramericana , clone “Nanlin 895”) its regulatory interactions with SHR/SCR network governing adventitious root (AR) morphogenesis secondary wall biogenesis. Transgenic overexpressing exhibited significant enhancement AR system architecture xylem development, manifesting increased cambial layers (1.5–2.2 fold) elevated lignin deposition (35% increase). Molecular analyses employing bimolecular fluorescence complementation (BiFC) quantitative real‐time PCR (qRT‐PCR) revealed that directly interacts PeSCR nucleus while transcriptionally upregulating PeSHR PeCYCD6;1 PeWOX5 expression. Transcriptomic profiling identified 817 differentially expressed genes (DEGs) between WT plants overexpression transgenic lines OE_PeRBR ), notable enrichment phenylpropanoid biosynthesis pathways. Key PAL 4CL CAD ) cellulose synthase CesA family members showed upregulation compared to WT. These findings establish as a central node within network, coordinating both development formation through transcriptional reprogramming regulators machinery woody species.

Language: Английский

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Resistance mechanism of Abies beshanzuensis under heat stress was elucidated through the integration of physiological and transcriptomic analyses

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 10, 2025

Language: Английский

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